General information for g16685.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 313b1.
Parent gene
- The parent gene g16685 is located on chr_4: 10503084..10504688 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16685 | g16685.t1 | isoform | g16685.t1 | 10503084 | 10504688 |
| chr_4 | g16685 | g16685.t1 | exon | g16685.t1.exon1 | 10503084 | 10503930 |
| chr_4 | g16685 | g16685.t1 | cds | g16685.t1.CDS1 | 10503084 | 10503930 |
| chr_4 | g16685 | g16685.t1 | exon | g16685.t1.exon2 | 10504081 | 10504688 |
| chr_4 | g16685 | g16685.t1 | cds | g16685.t1.CDS2 | 10504081 | 10504688 |
| chr_4 | g16685 | g16685.t1 | TSS | g16685.t1 | NA | NA |
| chr_4 | g16685 | g16685.t1 | TTS | g16685.t1 | NA | NA |
Sequences
>g16685.t1 Gene=g16685 Length=1455
ATGTCGCTGATTTTAATAATTTTAGTAGTAACTTTAACTTCACTTTTTGCAATTTTTCGC
TTCAAATATCGAAGATTTTATAAATTGGCAGCGAAAATTCCAAAACTTCCTGGTGGACTT
CCAATAATTGGAGCAACTCATATTTTTATGACAGCTGAACCAAAGGATTATTTGCCAATA
ATGTTGGATATGATTAAAAAAGACATGGTAATTGGATCAGTTTGGTTTGGGACACAATTT
GCTGTTATTTTAAATACAGTTGAAGATTTTCAAAAAGTTTTAACTTCACCAAATTGCATC
AATAAACCAAAACTTCTTTTTGATGCCTTCTTTTCTGATTCTGCAAGTTTTTGTGTTAAT
AATAATCCACATGAAATGAGAAGAAAACTTTTAAATCCATCATTTACAAGTTTGATGTTA
AATAAAACAAATTTTCATACAAATGAAGCAATTAAAGGATTTATAAATAAAATAAATGCA
AAAAGTGATGGAAAAACTATTGATATTTATCACGACATTGCTGCATGTGCTTTGAGAGGG
ACACTTCATGCTCATTTTGATTATGATGATGATTCACTTGATTATAAAGTTGTTAAAGGA
TTTGATGACTTCAAACCTTTAATAATGCAACGCTTAGGCAAACCATGGCTCTCATTCAAG
CCATTTTTCAAACTATCAAAACTCTCAAAACAAGTTGAAGCTGTTAGAACACCAATTTAT
GAATGCATCAACAAAATCATCGAAAGCAACAAACATCATCGTATTAAACTTGAAGGTCGT
TGTGTGTCAACAATTCGACAACTTTTAGATACAAAACAAAAATTATCAGATGAAGAAATA
GCTGAAGAAATTTTTATTTTTATTTATGCATCACATGAAACTTCAGCTTTAGCTTTATCA
GCTTGTCTTTTGCTTCTTGCAATGCATAAAAATGTTGAAGAAAAAATTTTTGAAGAAATT
AAAAGTGCAAAATTAAGTGAAGAATTCACGTCCATTAGTAATGAAGAAACACAAAGTTTT
CCATACATTGAAATGGTTATAAAAGAAACAATGCGACTTTTTCCACCAGTTGCTGTGACT
TTTAGGGAAACAATTGCTGAAGTTGAAGTTGGTGATTATTTAATTCCACCTGATACAATT
GTTGGTTTGCCAGTTTTATTAATTCATAGAAATGAAAAAATTTGGGGAAAAGATGCTGGA
AGTTTCAGACCAGAACGTTTTGAACCAGAAGAATTTAATAAAATTCCACAATTTGCATTT
TTACCTTTTCTGTTAGGAAAACGAACTTGCATTGGTTACAAATATGCAATGTATTTTATG
AAAATTTTTATTATAAATTTTGTAAAAAATTACAAAGTTGAAACAGATTTAAAATTTGAT
GAACTAAAAACTCAAATGACGCCAGCAACTTCACTTTTACAGGGCTATCCAATTAAAATT
ATTAAAAGGAAGTGA
>g16685.t1 Gene=g16685 Length=484
MSLILIILVVTLTSLFAIFRFKYRRFYKLAAKIPKLPGGLPIIGATHIFMTAEPKDYLPI
MLDMIKKDMVIGSVWFGTQFAVILNTVEDFQKVLTSPNCINKPKLLFDAFFSDSASFCVN
NNPHEMRRKLLNPSFTSLMLNKTNFHTNEAIKGFINKINAKSDGKTIDIYHDIAACALRG
TLHAHFDYDDDSLDYKVVKGFDDFKPLIMQRLGKPWLSFKPFFKLSKLSKQVEAVRTPIY
ECINKIIESNKHHRIKLEGRCVSTIRQLLDTKQKLSDEEIAEEIFIFIYASHETSALALS
ACLLLLAMHKNVEEKIFEEIKSAKLSEEFTSISNEETQSFPYIEMVIKETMRLFPPVAVT
FRETIAEVEVGDYLIPPDTIVGLPVLLIHRNEKIWGKDAGSFRPERFEPEEFNKIPQFAF
LPFLLGKRTCIGYKYAMYFMKIFIINFVKNYKVETDLKFDELKTQMTPATSLLQGYPIKI
IKRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g16685.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 483 | 3.3E-83 |
| 2 | g16685.t1 | PANTHER | PTHR24291:SF140 | CYTOCHROME P450 4C3-RELATED | 5 | 483 | 7.3E-65 |
| 3 | g16685.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 5 | 483 | 7.3E-65 |
| 8 | g16685.t1 | PRINTS | PR00385 | P450 superfamily signature | 290 | 307 | 2.8E-8 |
| 7 | g16685.t1 | PRINTS | PR00463 | E-class P450 group I signature | 299 | 325 | 2.7E-7 |
| 6 | g16685.t1 | PRINTS | PR00463 | E-class P450 group I signature | 344 | 362 | 2.7E-7 |
| 10 | g16685.t1 | PRINTS | PR00385 | P450 superfamily signature | 345 | 356 | 2.8E-8 |
| 5 | g16685.t1 | PRINTS | PR00463 | E-class P450 group I signature | 420 | 430 | 2.7E-7 |
| 9 | g16685.t1 | PRINTS | PR00385 | P450 superfamily signature | 421 | 430 | 2.8E-8 |
| 4 | g16685.t1 | PRINTS | PR00463 | E-class P450 group I signature | 430 | 453 | 2.7E-7 |
| 11 | g16685.t1 | PRINTS | PR00385 | P450 superfamily signature | 430 | 441 | 2.8E-8 |
| 1 | g16685.t1 | Pfam | PF00067 | Cytochrome P450 | 37 | 478 | 4.5E-67 |
| 16 | g16685.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 17 | g16685.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 18 | g16685.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 12 | - |
| 19 | g16685.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 15 | g16685.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 484 | - |
| 13 | g16685.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 37 | 483 | 5.37E-83 |
| 12 | g16685.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed