General information for g16732.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 305a1.
Parent gene
- The parent gene g16732 is located on chr_4: 10733251..10734189 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16732 | g16732.t1 | TTS | g16732.t1 | 10731461 | 10731461 |
| chr_4 | g16732 | g16732.t1 | isoform | g16732.t1 | 10731926 | 10734189 |
| chr_4 | g16732 | g16732.t1 | exon | g16732.t1.exon1 | 10731926 | 10732302 |
| chr_4 | g16732 | g16732.t1 | cds | g16732.t1.CDS1 | 10731926 | 10732302 |
| chr_4 | g16732 | g16732.t1 | exon | g16732.t1.exon2 | 10732425 | 10733251 |
| chr_4 | g16732 | g16732.t1 | cds | g16732.t1.CDS2 | 10732425 | 10733251 |
| chr_4 | g16732 | g16732.t1 | exon | g16732.t1.exon3 | 10733413 | 10733623 |
| chr_4 | g16732 | g16732.t1 | cds | g16732.t1.CDS3 | 10733413 | 10733623 |
| chr_4 | g16732 | g16732.t1 | exon | g16732.t1.exon4 | 10734093 | 10734189 |
| chr_4 | g16732 | g16732.t1 | cds | g16732.t1.CDS4 | 10734093 | 10734189 |
| chr_4 | g16732 | g16732.t1 | TSS | g16732.t1 | 10734423 | 10734423 |
Sequences
>g16732.t1 Gene=g16732 Length=1512
ATGGAAATATTTTTTGCTTCAATAATTTTATTTTTAATCATCGAGGGAATAATTTTAATT
GTAAAAGAATTTAAAAGACCTCAAAATTTTCCACCAGGTCCAAATTGGCTTCTTCCATTT
GTCGGCAGTTCCAAATACTTGAAGAAATTATCTCAAGAATTGGGTGGTCAGCATAAGGCC
TTTGATTACTTATCAAAAAAATACAACTCACCAATTTTCAGTATAAAACTTGGAAGAGAA
CTTGTAGTTGTTGTTAGAAGTTATGAATTAATAAAAGAAGTTCATTCAAGAGAAGAATTT
GATGGAAGACCGGATAATTTCTTTATGCGTCTTCGTACGATGGGCACACGTTTAGGCATC
ACTTGCACTGATGGTCGACTTTGGCAGGAACAAAGAAGTTTTGTTGTTCGTCAACTTCGA
AATGTTGGCTATGGAAAGTCAAAAATGGAAGAGCAAATTCATGTAGAACTGAATGAAATT
TTAGAAATTATCAGAAGTAGCAAAGACACTCCGATTTGGCCAGGTGAAACAAACTTTATA
GCAACAAGTGTGATAAATATTCTTTGGACTTTTACAACTGGTGCAAAAATCAAACGTAAT
GATGCAAGACTAATAAAATTTTTGAATTTACTTCAAAAACGATCAAAAGCATTTGAAATT
AGTGGTGGATATTTAAATCAAATGCCATGGTTGAGATTTTTTGCGCCAGAAGCGACTGGT
TATAATTTGGTTAAAAATCTTAATTCGGAATTTTACAAATTCTTTATGGAAGTCATCAAT
GAGCATTTAATGACATATAGTGAGGATAAATCAAATGAAGATCTCATTTATGCTTACATC
AAAGAAATGAATGAACAAAAAGACAAAGAAAATTCAACATTCAAAATTGATCAACTTGTG
ATGGTCATTTTAGACATTTTTATAGCTGGTGCAAGTTCAACTGGAATAACAATCGATTTA
GCTTTGATGTCAATGTTGATTTATCCAGAAATTCAAATGAAAGTTCAGAAAGAAATTGAT
GAAATTCTCAATGAAGACAGTGAAATGTTGAAATATTTTGATAAAAAGAAAATGCCATTT
ACAGAAGCAGTATTGCTTGAGGTTCAAAGATTTTATAGTATTGTTCCTATATCAGGACCA
AGAAGAATTTTAAAACCAACAACCTTAGCTGGTTACAATTTGCCCAAAGATACAACAGTT
TTAATCGGACTTGAAACAATTCACAAAGATGAAAATCATTGGAAAGATCCACAAAATTTC
AGACCAGAAAGATTTTTAGATGAAAAAAATAACATTTGCAATACTGAAAGACTTTTTCCA
TTTGGTGCTGGAAAAAGAAGATGTTTGGGTGATCAATTAGCTAAATCTTGTCTTTTTACA
TTTTTTGTTGAAATTTTGAAAGAATTTTCTTTGGAAAAATGTGAAAGTGATTTGCCTAGT
AATGATTTATTGCCGGGAATGCTATTGAGTCCAAAACCTTATAAAATTTTGTTTCGAAAG
AGAAAAAATTGA
>g16732.t1 Gene=g16732 Length=503
MEIFFASIILFLIIEGIILIVKEFKRPQNFPPGPNWLLPFVGSSKYLKKLSQELGGQHKA
FDYLSKKYNSPIFSIKLGRELVVVVRSYELIKEVHSREEFDGRPDNFFMRLRTMGTRLGI
TCTDGRLWQEQRSFVVRQLRNVGYGKSKMEEQIHVELNEILEIIRSSKDTPIWPGETNFI
ATSVINILWTFTTGAKIKRNDARLIKFLNLLQKRSKAFEISGGYLNQMPWLRFFAPEATG
YNLVKNLNSEFYKFFMEVINEHLMTYSEDKSNEDLIYAYIKEMNEQKDKENSTFKIDQLV
MVILDIFIAGASSTGITIDLALMSMLIYPEIQMKVQKEIDEILNEDSEMLKYFDKKKMPF
TEAVLLEVQRFYSIVPISGPRRILKPTTLAGYNLPKDTTVLIGLETIHKDENHWKDPQNF
RPERFLDEKNNICNTERLFPFGAGKRRCLGDQLAKSCLFTFFVEILKEFSLEKCESDLPS
NDLLPGMLLSPKPYKILFRKRKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g16732.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 21 | 503 | 4.5E-119 |
| 2 | g16732.t1 | PANTHER | PTHR24303:SF13 | CYTOCHROME P450 305A1-RELATED | 9 | 501 | 6.4E-197 |
| 3 | g16732.t1 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 9 | 501 | 6.4E-197 |
| 5 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 66 | 85 | 5.3E-46 |
| 8 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 183 | 201 | 5.3E-46 |
| 9 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 298 | 315 | 5.3E-46 |
| 12 | g16732.t1 | PRINTS | PR00385 | P450 superfamily signature | 309 | 326 | 2.9E-8 |
| 10 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 318 | 344 | 5.3E-46 |
| 4 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 362 | 380 | 5.3E-46 |
| 15 | g16732.t1 | PRINTS | PR00385 | P450 superfamily signature | 363 | 374 | 2.9E-8 |
| 11 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 403 | 427 | 5.3E-46 |
| 6 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 438 | 448 | 5.3E-46 |
| 13 | g16732.t1 | PRINTS | PR00385 | P450 superfamily signature | 439 | 448 | 2.9E-8 |
| 7 | g16732.t1 | PRINTS | PR00463 | E-class P450 group I signature | 448 | 471 | 5.3E-46 |
| 14 | g16732.t1 | PRINTS | PR00385 | P450 superfamily signature | 448 | 459 | 2.9E-8 |
| 1 | g16732.t1 | Pfam | PF00067 | Cytochrome P450 | 31 | 498 | 8.8E-94 |
| 20 | g16732.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 21 | g16732.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 22 | g16732.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 12 | - |
| 24 | g16732.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 19 | g16732.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 298 | - |
| 23 | g16732.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 299 | 328 | - |
| 18 | g16732.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 329 | 503 | - |
| 27 | g16732.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 441 | 450 | - |
| 16 | g16732.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 31 | 501 | 1.83E-97 |
| 26 | g16732.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 21 | - |
| 25 | g16732.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 299 | 321 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
