Gene loci information

Transcript annotation

  • This transcript has been annotated as Luciferin 4-monooxygenase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16741 g16741.t1 TSS g16741.t1 10771847 10771847
chr_4 g16741 g16741.t1 isoform g16741.t1 10771893 10773858
chr_4 g16741 g16741.t1 exon g16741.t1.exon1 10771893 10772030
chr_4 g16741 g16741.t1 cds g16741.t1.CDS1 10771893 10772030
chr_4 g16741 g16741.t1 exon g16741.t1.exon2 10772093 10772300
chr_4 g16741 g16741.t1 cds g16741.t1.CDS2 10772093 10772300
chr_4 g16741 g16741.t1 exon g16741.t1.exon3 10772482 10773486
chr_4 g16741 g16741.t1 cds g16741.t1.CDS3 10772482 10773486
chr_4 g16741 g16741.t1 exon g16741.t1.exon4 10773554 10773858
chr_4 g16741 g16741.t1 cds g16741.t1.CDS4 10773554 10773858
chr_4 g16741 g16741.t1 TTS g16741.t1 10773938 10773938

Sequences

>g16741.t1 Gene=g16741 Length=1656
ATGCAAACTGAAAATGATCCAAATATTTTATATGGCGGTGACTATAGTGAATCAAATGAG
CCATTAGATGAAAATCGTGGATCACTTGGTGCTGTGATTTTAAATGATCTTTTACTCGGT
GGTGATCAAACTTTATTTATTAGTGGAGTGACCGATAAAAAATGGACTTATAATGATGTT
CGTGTTGAATCAATGAAAGCTGCAAGAATTCTTTATGGTGTTGGATTACGTCAAAATGAT
TCAATTGCAATTCTTAGTGAAAACCGAATTGAATTTCCGATAATTTCTTTTGGTGCTCTT
TATTTGAATGCTGTTGTTGCTCCAATTAATGTCACTTATACTGAAAGAGAGCTCACACAT
GCTTTAAATTTATCAAGACCAAAAATTGTTTTTACTTCGGCATTTGCAGCTAAATTAGTT
ACATCTGTATGCAACAAATTAGATTTTGTCAAAACAATTGTTCATTTAGATGGAAATAAA
AGTGAAGGCAAAATAAAATCAATGAATGATTTTATAAAAAATGAGGATAAAAATTTCAAT
GTTGAAGCAAATGCGAGTAAAAAAGTTGACATAAAAAATCAACTTTCATTCATCATGATG
TCATCAGGAACGACTGGAATTGCAAAAGGAGTGCAACAAACACAAGAAAATGTTTTGAGT
ATAATTCAAAGTTATCGTGACATGTTTGTGTTATTTAGAATGCTTCATGATACAAAATCT
ATAATTTTAAATATTGCACCATTCTTTCATGGATTTGGTTTCATTTCAATGTTGTTAATT
TCATGCTCAAGAGAAGCAACTGCTGTTTTTCTACCAAGATTTGAAGAAAATGCATTTTTT
AAAGCAATTGAGAAGCATAAAGTCAATATCATCACTGTTGTTCCACCTATTATGGTTTTA
CTTGCTAAAAGTCCACTTGTTGATAAATATGACTTATCAAGTATTAAAGACATCGGAAGT
GGTGCAGCACATTTGTCAGTAGAAATTGAAGAGCAAGTTATGAAAAGATTCAAAGGACAA
GTGACGATAAGACAAGGTTATGGCATGACAGAATCAGTTTTTGGCACTTTAGGTGGAACT
GTTTTACTTAAACCTGGTTCTGTTGGTCAACCTATGAAAGGCGTCTATGCGAAAGTAATT
GATGAAAATGGAAATTCATTGGGACCAAATAAAGTTGGTGAATTGTGCTTTAAAAGCACT
CGAATTATGAAAGGATATGTCAATGATGAAAAAGCAACAAGTGAAATTATTGACAAAGAT
GGTTGGTTGCATACTGGCGACTTGGGATATTATGATGACGATTTTCAATTTTTCATTGTT
GATCGATTGAAAGAGTTGATTAAATACAAAGCTTTTCAGGTTCCACCAGCAGAACTTGAA
GCTCTTTTGTTGAAAAATCCTAAAATTAAAGACGTTGGAGTTATTGGAATTCCTGATGAA
GTTGCTGGTGAACTTCCTTTTGCATTTGTTGTGAAGCAACCTGGAGTTGAACTAACAGAA
GAAGAAGTAAAAGATTTTGTTGCTAAAAATGCAAGCAATGCAAAATGGCTAAGAGGTGGC
GTGAAATTTATTGATGAAATCCCTAAAAATTTGAGTGGAAAAATTTTAAGAAAAGACTTG
AGAGATTTGTATAAAAATACAAAATCTAAATTGTAA

>g16741.t1 Gene=g16741 Length=551
MQTENDPNILYGGDYSESNEPLDENRGSLGAVILNDLLLGGDQTLFISGVTDKKWTYNDV
RVESMKAARILYGVGLRQNDSIAILSENRIEFPIISFGALYLNAVVAPINVTYTERELTH
ALNLSRPKIVFTSAFAAKLVTSVCNKLDFVKTIVHLDGNKSEGKIKSMNDFIKNEDKNFN
VEANASKKVDIKNQLSFIMMSSGTTGIAKGVQQTQENVLSIIQSYRDMFVLFRMLHDTKS
IILNIAPFFHGFGFISMLLISCSREATAVFLPRFEENAFFKAIEKHKVNIITVVPPIMVL
LAKSPLVDKYDLSSIKDIGSGAAHLSVEIEEQVMKRFKGQVTIRQGYGMTESVFGTLGGT
VLLKPGSVGQPMKGVYAKVIDENGNSLGPNKVGELCFKSTRIMKGYVNDEKATSEIIDKD
GWLHTGDLGYYDDDFQFFIVDRLKELIKYKAFQVPPAELEALLLKNPKIKDVGVIGIPDE
VAGELPFAFVVKQPGVELTEEEVKDFVAKNASNAKWLRGGVKFIDEIPKNLSGKILRKDL
RDLYKNTKSKL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g16741.t1 Gene3D G3DSA:3.40.50.980 - 53 364 4.4E-118
7 g16741.t1 Gene3D G3DSA:3.40.50.980 - 57 214 4.4E-118
10 g16741.t1 Gene3D G3DSA:2.30.38.10 Luciferase; Domain 3 365 442 4.4E-118
9 g16741.t1 Gene3D G3DSA:3.30.300.30 - 443 550 6.6E-29
3 g16741.t1 PANTHER PTHR24096:SF353 GH16244P-RELATED 19 550 1.7E-161
4 g16741.t1 PANTHER PTHR24096 LONG-CHAIN-FATTY-ACID–COA LIGASE 19 550 1.7E-161
2 g16741.t1 Pfam PF00501 AMP-binding enzyme 43 449 1.5E-89
1 g16741.t1 Pfam PF13193 AMP-binding enzyme C-terminal domain 458 534 1.8E-14
11 g16741.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 240 -
13 g16741.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 241 260 -
12 g16741.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 261 551 -
6 g16741.t1 ProSitePatterns PS00455 Putative AMP-binding domain signature. 198 209 -
5 g16741.t1 SUPERFAMILY SSF56801 Acetyl-CoA synthetase-like 40 546 8.5E-125

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values