General information for g16883.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g16883 is located on chr_4: 11598180..11601238 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16883 | g16883.t1 | isoform | g16883.t1 | 11598180 | 11601238 |
| chr_4 | g16883 | g16883.t1 | exon | g16883.t1.exon1 | 11598180 | 11599160 |
| chr_4 | g16883 | g16883.t1 | cds | g16883.t1.CDS1 | 11598180 | 11599160 |
| chr_4 | g16883 | g16883.t1 | exon | g16883.t1.exon2 | 11599221 | 11600260 |
| chr_4 | g16883 | g16883.t1 | cds | g16883.t1.CDS2 | 11599221 | 11600260 |
| chr_4 | g16883 | g16883.t1 | exon | g16883.t1.exon3 | 11600353 | 11601238 |
| chr_4 | g16883 | g16883.t1 | cds | g16883.t1.CDS3 | 11600353 | 11601238 |
| chr_4 | g16883 | g16883.t1 | TTS | g16883.t1 | 11601294 | 11601294 |
| chr_4 | g16883 | g16883.t1 | TSS | g16883.t1 | NA | NA |
Sequences
>g16883.t1 Gene=g16883 Length=2907
ATGACAAGCAGCAAAACGGTTTTTCTTTTGCTATTTCTTTCAGTAACATCGACGTGGCAA
AGTCCAATTGAAGAAAAAGAAACAAAAAATTGGAAACTAAAAAGTGAACAAAATTTACAA
ATTTCAATTGATGATGAAGAAATCACAACTTATCGTTTACCTAACAACACTTTACCAGTT
CGTTATGACATTTGGTTGCAAACTGATGTCGATAAAGCAAATTTTGCATTTGAAGGTCGT
GTGAAAATTCAAATTCAAGCAGTAGAAGCAACAGATACCGTGACTTTACATTTTCTAGAC
ATAACAATTAAAAATATTGATCATATTGATGGAAGTGGAAATATTTTAAATTCTTCATTA
GCATTTGAACTAATTCAGCCTTATGAATTTTTAAAAATCGCACTACCACGTCAGATGACC
ATCAATGAAGAATTTATTTTACACATTTCCTACAATGGCACACTTAAAATTTCAAATGAA
GGATTTTATCGGGCATATTATGTTGATTATACAACACCAAATAATGATATAATTTATTAC
GCTGTTACACAATTTGAATCAACTGATGCACGTCACGCATTTCCATGTTATGATGAACCT
GGAATTAGAGCTCCAATTGGATTGCAAATACAACATGATAGAAGTTACACAGCTTATTCC
AATATGCCAGTTATCACAATCAATGAAGTTCCTGGAACAAATTATGTCACAACTGTTTTT
GAAGATACACCAAAAATGCAAACTTACCTTTTAGCATTTCTTGTGTCACCATTTAAATAC
ATTGCAAATAATGACACTGTTTTTCCTCAAAAAATTCTTGCAAAACCTTCATCAATTGAT
AATGGTGAAGCTGATTATGCAGCTATGATTGCTGATCCAATTTTGAAAAAATTCATAAAT
CATCTTGATGTTAATTTTACATTACCAAAATTAGATCATGCAGCTATAACACAATTTGCA
GCTGGTGCTATGGAAAATTGGGGTTTAATAACATACCGTGAAAGTGCATTACTCTTAAGT
ACATCATATAGTGAAAGTCAAATTGCCTATTATCGAATGTATATTGCTGAAGTTATTGCA
CATGAAATCGCACATCAATGGACTGGTAATCTTGTTTCTCCATCATGGTGGACAGATTTA
TGGTTGAATGAAGGTTTTGCAACTCTTTATGAATGTTACATTCCACATTTGTTATGGCCT
AATGATGGATATGATGAAGATTGTAGAATTGCAAATCTTGCAATAGCACTTCGCAATGAT
TTACCAACAAGTCGTTTTTCTGTTCCAATGTCATATTATGTTGAAACACCAGTAGCAATT
GATGGACGATTTAGTTTTGTTTCTTATGAAAAATCAGGATCAGTTTTAAAAATGTTTCAA
GAGGCACTTTCAGTTGAAACTTTCACAAAAGGAGTAAGTCAATATTTGAAGAAAATGAGC
TTTAAAGCTGCTGTACCTGATGATTTATTTTCATCATTACAAGAAGCTTGTGATGAAGAT
CAACCTGGAAATGGATTAGATATTGCATCAATTATGAAAACTTGGGTTTATCAAGCTGGT
TATCCTTTAATTTCACTTACAAAATCTGGTCAAAATCTAACAATTAGTCAAAATCGTTAT
CCAAATGCTTCAGAAGTTTATTCAGTTCCAATTTCATTTGCAACGAAATCAAATCCTGAT
TTTGAAAATAAAAAAGTTGGTCTTTGGTTGCATGACAGTTCAATTACTTTGCCATTTAAT
GCTTTTGGAATGGATGAAAATGATTGGATAATTTTTAATATTCAACAAACTGGTTATTAC
CGCATTGCATATGATTCTAATTTATGGGCTGCAATTGCAAGAGATTTAAATGAAAATCAC
AATGCAATCCATTTAATTAATAGACGAGTCTTGCAAGATGAGCTCAATATTGGCTATAAC
ACGCTTGCTACACTTCATGCATCTGATATTTTTGAATTTGAAAGTTATCTAAAATCAGAA
AATAATTATTTAGTATGGAATGATGCTGGAAATATTTTAAGATCTCTCAACACGTCACTT
TTTAATACTGAAATTTATCCACGCTATCTTGAGTTTATCAAAACAATTTCACATTCACAT
ATGGAAAATGTTGGTCTTGAAGCAACTGATGGTGAAACTGCAGATATTACCCAGTTAAGA
ATAAGAGTGAAAACTTTCAATTGTTATGCATTTGACGAAGATTGTCTTGCACATGAGCAT
CAAAAATTAATAACTTACATGAGAAATAGCACAGAAAATCCAATTCCTGATTTTTGTTCT
GGTTTCCGTCATGCAAACAGCACAATTTATGCTTATTATCTTGAGCAATTGGCTGATAAT
TCTGCCTTGATCAATCGAAATCGAATTGTGTCAAACATTGGCTGTTCATTGAATAAAAAT
TTGCTTGGTCTTGTTGTAGAATTAGTTGAAGATTCTGCAAATGTTTTGACATCAAGTGAA
AGGCTAACGATCATCCGCAATATGTTGACAACTAGCACAGTTGGTTTTGAAGTTGCTTTT
GCTTACATTGGACGAAATATTGAAAGCATTTCTTCATATTTAACTCAATTATCTACAACA
GTCAACACAAATTCAACAGCTGATCAATTAACAAAAATTTTAAATGATGCAGTTGCTGAA
GGGTTTTTGACAGAAGCAAATTCATTGAATCTTAAAAATTCTATAAATGCAAATTTAGCA
TGGCAAAAAAAGCACTATGATTCAGTAGTTAATTGGTTTAAAAATATTGATGAAACAACT
TTAGCACCAACAACAACTACTGATAATTTTACAGAAACAGCAGATGATTCAACAACAACT
CCAGTAGCAACAACTACTAACAGTGGGGCTTCAATTACTGCATCATTTATTCTTACAGCT
TTTTTGTTTTTTGTAACATTCAAGTAA
>g16883.t1 Gene=g16883 Length=968
MTSSKTVFLLLFLSVTSTWQSPIEEKETKNWKLKSEQNLQISIDDEEITTYRLPNNTLPV
RYDIWLQTDVDKANFAFEGRVKIQIQAVEATDTVTLHFLDITIKNIDHIDGSGNILNSSL
AFELIQPYEFLKIALPRQMTINEEFILHISYNGTLKISNEGFYRAYYVDYTTPNNDIIYY
AVTQFESTDARHAFPCYDEPGIRAPIGLQIQHDRSYTAYSNMPVITINEVPGTNYVTTVF
EDTPKMQTYLLAFLVSPFKYIANNDTVFPQKILAKPSSIDNGEADYAAMIADPILKKFIN
HLDVNFTLPKLDHAAITQFAAGAMENWGLITYRESALLLSTSYSESQIAYYRMYIAEVIA
HEIAHQWTGNLVSPSWWTDLWLNEGFATLYECYIPHLLWPNDGYDEDCRIANLAIALRND
LPTSRFSVPMSYYVETPVAIDGRFSFVSYEKSGSVLKMFQEALSVETFTKGVSQYLKKMS
FKAAVPDDLFSSLQEACDEDQPGNGLDIASIMKTWVYQAGYPLISLTKSGQNLTISQNRY
PNASEVYSVPISFATKSNPDFENKKVGLWLHDSSITLPFNAFGMDENDWIIFNIQQTGYY
RIAYDSNLWAAIARDLNENHNAIHLINRRVLQDELNIGYNTLATLHASDIFEFESYLKSE
NNYLVWNDAGNILRSLNTSLFNTEIYPRYLEFIKTISHSHMENVGLEATDGETADITQLR
IRVKTFNCYAFDEDCLAHEHQKLITYMRNSTENPIPDFCSGFRHANSTIYAYYLEQLADN
SALINRNRIVSNIGCSLNKNLLGLVVELVEDSANVLTSSERLTIIRNMLTTSTVGFEVAF
AYIGRNIESISSYLTQLSTTVNTNSTADQLTKILNDAVAEGFLTEANSLNLKNSINANLA
WQKKHYDSVVNWFKNIDETTLAPTTTTDNFTETADDSTTTPVATTTNSGASITASFILTA
FLFFVTFK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 26 | g16883.t1 | CDD | cd09601 | M1_APN-Q_like | 60 | 517 | 2.17765E-165 |
| 16 | g16883.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 43 | 258 | 3.4E-58 |
| 17 | g16883.t1 | Gene3D | G3DSA:1.10.390.60 | - | 266 | 520 | 5.9E-75 |
| 15 | g16883.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 521 | 604 | 3.8E-17 |
| 18 | g16883.t1 | Gene3D | G3DSA:1.25.50.20 | - | 605 | 916 | 5.3E-36 |
| 4 | g16883.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 39 | 918 | 8.8E-195 |
| 5 | g16883.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 39 | 918 | 8.8E-195 |
| 10 | g16883.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 191 | 206 | 3.0E-28 |
| 6 | g16883.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 240 | 255 | 3.0E-28 |
| 8 | g16883.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 319 | 329 | 3.0E-28 |
| 7 | g16883.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 358 | 373 | 3.0E-28 |
| 9 | g16883.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 377 | 389 | 3.0E-28 |
| 3 | g16883.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 59 | 250 | 8.2E-42 |
| 2 | g16883.t1 | Pfam | PF01433 | Peptidase family M1 domain | 287 | 515 | 8.9E-58 |
| 1 | g16883.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 589 | 858 | 1.3E-28 |
| 21 | g16883.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 22 | g16883.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 23 | g16883.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 15 | - |
| 25 | g16883.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 20 | g16883.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 948 | - |
| 24 | g16883.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 949 | 967 | - |
| 19 | g16883.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 968 | 968 | - |
| 12 | g16883.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 49 | 257 | 8.76E-47 |
| 11 | g16883.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 270 | 525 | 2.93E-64 |
| 14 | g16883.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 13 | g16883.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 25 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed