Gene loci information

Transcript annotation

  • This transcript has been annotated as 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16960 g16960.t4 TSS g16960.t4 11877931 11877931
chr_4 g16960 g16960.t4 isoform g16960.t4 11878813 11880536
chr_4 g16960 g16960.t4 exon g16960.t4.exon1 11878813 11878869
chr_4 g16960 g16960.t4 cds g16960.t4.CDS1 11878814 11878869
chr_4 g16960 g16960.t4 exon g16960.t4.exon2 11879016 11879121
chr_4 g16960 g16960.t4 cds g16960.t4.CDS2 11879016 11879121
chr_4 g16960 g16960.t4 exon g16960.t4.exon3 11879327 11879479
chr_4 g16960 g16960.t4 cds g16960.t4.CDS3 11879327 11879479
chr_4 g16960 g16960.t4 exon g16960.t4.exon4 11879833 11880536
chr_4 g16960 g16960.t4 cds g16960.t4.CDS4 11879833 11879907
chr_4 g16960 g16960.t4 TTS g16960.t4 NA NA

Sequences

>g16960.t4 Gene=g16960 Length=1020
TGATATTTTAAGAGTTGGTGGTGATGTGACATTAATTGGATGGGGAACACAAGTCCATGT
TTTAAGAGAAACTGCCGAATATGCAAAAAGAAAACTCAATGTTAATTGTGAAGTTATTGA
TTTAGTTTCTATTTTGCCATGGGATGTTGAGACTGTTTGCAATTCAGTAAAGAAGACTGG
TCGTTGTATTATTGCACATGAGGCTCCAGTAACAAATGGCTTTGGTGCAGAAATTGCAGC
TACAGTTCAAGAAGAATGTTTCTTGCATTTAGAAGCACCGATTCAAAGAGTTTGCGGATG
GGATACTCCGTTTCCGCATGTTTTTGAACCATTTTATCTTCCTGATAAATATCGATGCTT
AGAAGCAATTAAAAAAGTAATTGACTATTAAAACTTTGGTGCAAGATAAAAATTAATTTG
AACCCAACGTACAAAAATAATTTTTAAAACACTTAAAATTCTTTGGTTTAAATTTTTTGT
ACATAATTTTACATTCAAACAGTGTCCGAAATTATTTTAAATTTATTTACAAGTAAATAA
AAATTAAGTCAATTTTTTCGAGTAAAAAAATAAATTTTACTTAATTTTTAGTAACTCTTA
AATAAAATTTAAAACGATTTCGGACTTTGCTTTCAAAAATGAAATTTTACAAAAAATTTT
GATGGAAGAATTTTAAGTTTTGCATTTATTGAAATTTACTGGGAAATTATGAAAAAATAA
ATTTTTGTGTAAAAAATTCATGAAATATAACTGAAATATTGAAACTTGTTGCATTTATTA
ATTTTAAAATGAGAAAATTATAAATTTTGAGAATTTTCAAAAGATTTGTTAAAAAAAATG
TTGATGTATTTTATTGTAACAATAAAAACCGTTTAAAAAAAGGAAAAAAAATCAAGAATT
TTTTTTATTTTTCAAGGAAATAAAAACAAAAAATTGAATTTTTGATAAAAAGACTAAATT
TTATTCAAATTTTACTTTTTTAATCATTTTTGGAACAAAAATATTTTAAAATCTGTATTT

>g16960.t4 Gene=g16960 Length=129
DILRVGGDVTLIGWGTQVHVLRETAEYAKRKLNVNCEVIDLVSILPWDVETVCNSVKKTG
RCIIAHEAPVTNGFGAEIAATVQEECFLHLEAPIQRVCGWDTPFPHVFEPFYLPDKYRCL
EAIKKVIDY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
4 g16960.t4 Gene3D G3DSA:3.40.50.920 - 1 125 0
2 g16960.t4 PANTHER PTHR42980 2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED 1 128 0
1 g16960.t4 Pfam PF02780 Transketolase, C-terminal domain 2 116 0
3 g16960.t4 SUPERFAMILY SSF52922 TK C-terminal domain-like 2 129 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values