Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1697 g1697.t1 TTS g1697.t1 12590765 12590765
chr_3 g1697 g1697.t1 isoform g1697.t1 12591648 12592765
chr_3 g1697 g1697.t1 exon g1697.t1.exon1 12591648 12592258
chr_3 g1697 g1697.t1 cds g1697.t1.CDS1 12591648 12592258
chr_3 g1697 g1697.t1 exon g1697.t1.exon2 12592321 12592499
chr_3 g1697 g1697.t1 cds g1697.t1.CDS2 12592321 12592499
chr_3 g1697 g1697.t1 exon g1697.t1.exon3 12592563 12592765
chr_3 g1697 g1697.t1 cds g1697.t1.CDS3 12592563 12592765
chr_3 g1697 g1697.t1 TSS g1697.t1 12592809 12592809

Sequences

>g1697.t1 Gene=g1697 Length=993
ATGGCTTTTTTAATTTATATTGTTTTGGCAATTTTAAGCTATATTGCTTATAAGAAGATC
ATACGAAACCATGATTATTTTGAGAAGAAAGGGTTAAAATTCAGTAAACCAATAGCTTTT
ATTGGTTCAAATAATTTGCTTTTGAAAAAATGTTCATTACCAGAGACAGTTCTCGCATGG
TACAATGAGTTTAAAAGTGAAAAAATTTACGGAATGTTCAATTTCATGAAACCAGTATTC
ATTATACGCGACCCACAAATAATTAAAAAATTGGCAGTTAAAGATTTTGATTTTTTCACT
GATCATCGTGTGATTATTTCTGAGGAAGTAGATCCGTTATTTGGAAAAGCTTTAATTTCT
TTACAAGGACAAAAATGGAAGGATATGCGTTCAACATTATCACCGGCATTCACAGGTCAT
AAAATGCGACTAATGTTTGATTTTGTGACTAAAGTAGGAAAACAAACAGCTGAGACACTG
AAGAGACAAGTTGATGAAGGTCGTAAGAATGATTTTGAGTTCAAAGAACTTGCGACGAAA
TTCACTGTTGATAATATTGCTTCTTGTGCTTTTGGGATATCGATAAATAGCTTTTCTAAT
CCTGAAAATGACTTCTTTGATATTGCAAAAAAGTTTGCAAACTTTGGTAATACTAAAACA
GTTTTGAAGTTCATTGGATATATGGTGATGCCATCAATCATGAAATTTTTCAAAATAAAA
GTGTTTGGTGCTAAAATAAGTAAATTTTTCAAAGAAGCTGTTTTGGATACTATAAAAGTG
AGAGAAGAAAAAGGAATAGTCAGACATGATATGATTAATCTTCTAATTCAAGCTAAGAAA
GGCACTTTAGTGCATGAAATTGAAGAAAAATTAATAGAAGGATTTGCAACAGTTGAAGAG
TCACAAATGGGAAAAATTCAATCAAAGAGAAAATGGGATGATGAAGACCTTGCAGCACAA
GCTTTCATCTTTTTCCTAGCTTTAAGTTTTTAA

>g1697.t1 Gene=g1697 Length=330
MAFLIYIVLAILSYIAYKKIIRNHDYFEKKGLKFSKPIAFIGSNNLLLKKCSLPETVLAW
YNEFKSEKIYGMFNFMKPVFIIRDPQIIKKLAVKDFDFFTDHRVIISEEVDPLFGKALIS
LQGQKWKDMRSTLSPAFTGHKMRLMFDFVTKVGKQTAETLKRQVDEGRKNDFEFKELATK
FTVDNIASCAFGISINSFSNPENDFFDIAKKFANFGNTKTVLKFIGYMVMPSIMKFFKIK
VFGAKISKFFKEAVLDTIKVREEKGIVRHDMINLLIQAKKGTLVHEIEEKLIEGFATVEE
SQMGKIQSKRKWDDEDLAAQAFIFFLALSF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g1697.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 15 329 2.5E-52
2 g1697.t1 PANTHER PTHR24292 CYTOCHROME P450 4 327 1.0E-86
3 g1697.t1 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 4 327 1.0E-86
5 g1697.t1 PRINTS PR00464 Group II E-class P450 signature 123 143 3.6E-9
4 g1697.t1 PRINTS PR00464 Group II E-class P450 signature 180 198 3.6E-9
1 g1697.t1 Pfam PF00067 Cytochrome P450 64 326 7.1E-20
9 g1697.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 18 -
10 g1697.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
11 g1697.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
12 g1697.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 18 -
8 g1697.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 19 330 -
6 g1697.t1 SUPERFAMILY SSF48264 Cytochrome P450 37 327 9.3E-33

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values