Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1699 g1699.t1 isoform g1699.t1 12594110 12595235
chr_3 g1699 g1699.t1 exon g1699.t1.exon1 12594110 12594729
chr_3 g1699 g1699.t1 cds g1699.t1.CDS1 12594110 12594729
chr_3 g1699 g1699.t1 exon g1699.t1.exon2 12594788 12594966
chr_3 g1699 g1699.t1 cds g1699.t1.CDS2 12594788 12594966
chr_3 g1699 g1699.t1 exon g1699.t1.exon3 12595027 12595235
chr_3 g1699 g1699.t1 cds g1699.t1.CDS3 12595027 12595235
chr_3 g1699 g1699.t1 TSS g1699.t1 12595266 12595266
chr_3 g1699 g1699.t1 TTS g1699.t1 NA NA

Sequences

>g1699.t1 Gene=g1699 Length=1008
ATGGGTCTTTTTATGTGTTTGATTATATTAGCTGCTACTTTGATTGCATATAAACTTCTT
TTTGCCGGAAAAAATAAAAACTATTTCCTAAAGAAAGGAGTTAAATATGAGAAGCAAAAT
GTTCTTATAGCTATAAAAAATATGTTCACTCAGAAAAAATCATTTCCTGAAATTGCGAAT
GGCTGGTATAATGATTTTAAAGAAGAAAAAATATCGGGTATTTTTGAATTTTCAAGGCCA
ATTTTTATCATACGCGACCCACAACTGATTAAGAGAATGACAGTTAAAGATTTTGATTTT
TTTACTGATCATCGTGTGATCATTACTGAAAATAATGATATATTATTGGGGAAATCTTTG
ATTTCCTTATGTGGACAAAAATGGAAAGATATGCGTTCAACGTTATCACCGGCATTCACA
GGTCATAAAATGCGACTGATGTTTGATTTTGTTGCAACTGTTGGAAAACAAACTGTTGAA
ACCTTGAAAAAGCAAATTAACGAAGGTCATGGAAATGATTTTGAATTTAAAGAGCTTGCG
ACGAAATTCACTGTCGATAATATTGCTTCATGCGCTTTTGGGATATCGGTAAACAGTTTT
TCTGATCCCAAAAACGATTTTCATCGTATTGCAAATGAATTAACAGATTTTCAAAATTTC
AAGTCTATTATGAAATTTATGGGCTATTTGATAGTACCATGGTTAATGAATTTTTTCAAG
ATAAGCTTTTTCAGTGAAAAAGTGAGTAAATTCTTTGAAGAGGCGATTTTGGATACTATG
AAGATTAGAGAAGAAAAAGGAATAGTCAGACATGATATGATTAATCTTCTAATTCAAGCT
AAGAAAGGCACTTTAGTGCATGAAATTGAAGAAAAATTAATAGAAGGATTTGCAACAGTT
GAAGAGTCACAAATGGGAAAAACTCAATCAAAGAGAAAATGGGATGATGTAGACTTAGCT
GCACAAGCTTTCATCTTTTTCTTTGCTTTAAGTATCAAATCTTTCTAA

>g1699.t1 Gene=g1699 Length=335
MGLFMCLIILAATLIAYKLLFAGKNKNYFLKKGVKYEKQNVLIAIKNMFTQKKSFPEIAN
GWYNDFKEEKISGIFEFSRPIFIIRDPQLIKRMTVKDFDFFTDHRVIITENNDILLGKSL
ISLCGQKWKDMRSTLSPAFTGHKMRLMFDFVATVGKQTVETLKKQINEGHGNDFEFKELA
TKFTVDNIASCAFGISVNSFSDPKNDFHRIANELTDFQNFKSIMKFMGYLIVPWLMNFFK
ISFFSEKVSKFFEEAILDTMKIREEKGIVRHDMINLLIQAKKGTLVHEIEEKLIEGFATV
EESQMGKTQSKRKWDDVDLAAQAFIFFFALSIKSF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g1699.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 18 331 2.7E-50
2 g1699.t1 PANTHER PTHR24292 CYTOCHROME P450 5 329 9.9E-89
3 g1699.t1 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 5 329 9.9E-89
4 g1699.t1 PRINTS PR00464 Group II E-class P450 signature 125 145 3.0E-9
5 g1699.t1 PRINTS PR00464 Group II E-class P450 signature 182 200 3.0E-9
1 g1699.t1 Pfam PF00067 Cytochrome P450 69 290 1.0E-20
9 g1699.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 22 -
10 g1699.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 5 -
11 g1699.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 6 17 -
12 g1699.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 18 22 -
8 g1699.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 23 335 -
6 g1699.t1 SUPERFAMILY SSF48264 Cytochrome P450 47 329 8.38E-33

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values