Gene loci information

Transcript annotation

  • This transcript has been annotated as Glutamine-dependent NAD(+) synthetase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g17053 g17053.t5 isoform g17053.t5 12321475 12323816
chr_4 g17053 g17053.t5 exon g17053.t5.exon1 12321475 12322589
chr_4 g17053 g17053.t5 cds g17053.t5.CDS1 12321475 12322589
chr_4 g17053 g17053.t5 TTS g17053.t5 12321495 12321495
chr_4 g17053 g17053.t5 exon g17053.t5.exon2 12322654 12323334
chr_4 g17053 g17053.t5 cds g17053.t5.CDS2 12322654 12323260
chr_4 g17053 g17053.t5 exon g17053.t5.exon3 12323412 12323816
chr_4 g17053 g17053.t5 TSS g17053.t5 NA NA

Sequences

>g17053.t5 Gene=g17053 Length=2201
ATGGGTCGAAAAGTAACAGTTGCAGTATCTACATTGAGTCAATGGGCTTTGGATTTTGAA
GGCAACATGGCAAGAATTATACAATCAATTTTGGAAGCACGTGAACTTGGAGCTCTTTAT
CGTACTGGACCAGAATTGGAAATTACGTAAGAACTTTTAAAAATAGAATTTTTTGATAAA
CTTTTAAATTTTTTTAAAGTGGTTACAGTTGTGAAGATCATTTTTATGAGCCAGATACAT
TCTTGCATTCATGGGAAGTTCTTTTGCAAATTATGATGTCACCAGTTTGTGAAAATATGC
TGATTGATGTTGGAATGCCAGTTCAACATTCTAATGTTGCTTACAATTGTCGTGTGGTTT
TTTATAATAAAAAAATTATTCTCATTAGACCTAAAATGATGATGTGAAACTATCGTGAAT
CTCGTTGGTTCACAGCATGGACAAAAGTAAGAAAAGTTGAAGATTATTTCCTTCCACGAA
TGATTGCGATAGCTACAAATCAACTTACGGTTCCTTTTGGTGATGGTGTGATTTCAACAA
GAGAAACTTGCATTGGTTTTGAAATATGCGAAGAATTATGGAATCCACGTTCAACACATA
TCGATTTGGGGTTAGCAGGTGTTGAAATTATTGCCAATGGCAGTAGCAGTTATATTCAAC
TACGTAAAGCTCATATCACAGCAGATTTAATAAAAAATGCAACATATAAAGCTGGTGGTG
CTTATCTTTTTTCAAATTTACGTGGATGTGATGGACAACGAGTTTGGTGGGGCGGAAATT
CAGCAATTGGATTAAATGGTGAAATAATTGCAAAAAGCAAGCAATTTTCATTGTTGGATG
TTGAAGTTACAGTAGCAACTATTGATTTAGAAGACATCAGAAGTTATCGAATGGCTTTAA
GATCACGTTGCACTTTTGGAGCTTCTAGTCCAGTTTATCCAAGAATTGCTGTCGATTGTG
AACTTTCAAGTCGTCGCGATGTTTTGATTCCTGTGAATTTACCATTAGAATGGATTAATC
ATACTCCAGAAGAAGAAATTGCTATGGGTCCTGCATGCTGGTTGTGGGATTATCTTCGTC
GTTCAGGTCAAGGTGGTTATTTTTTGCCATTGTCAGGCGGTATTGATTCTTGCAGTACAG
CATGCATTGTGTATAACATGTGTCAAATGGTAGTTGATGCAATTCAAAATGGCGAAGATG
ACGTTTTACGTGATGTAAGAAAAATCACAGCTGATCCAGAATTTTCACCTCAAACAGCAG
CTGAATTGTGCAATAAAATATTTGTGACTTGTTTTATGGGATCAAAAAATTCTAGTAAAG
AAACAAAAAATCGTGCAGCAGTTTTAGCAAATGAAATTGGAAGTTATCATTTGGAACTCA
ATATTGACAGTGTTTTTGACACAATTTTATCAGTTTTCAAAGCAGTCACTGGTTTTATAC
CAAAATATCGGGTTCAAGGTGGTTCCACAAGAGAAAATCTTGCATTGCAAAACATTCAAG
CAAGAGTTCGAATGGTGCTGTCTTATCTTTTTGCACAATTAGTTTTATGGACAAGAAATC
GTCCAGGTGGTCTTTTAGTCCTTGGTTCTGCAAATGTTGATGAATCATTGCGTGGTTATA
TGACAAAATATGACTGCTCTTCAGCTGACGTAAATCCAATTGGTGGAATTTCAAAAACTG
ACTTGAGAAGATTTTTAAATTATGCCATGAAAAAATATAATATACCAATTTTAAATGAAA
TATTTTTGGCTCAACCAACAGCTGAACTTGAGCCACTTCATGAAGGACAATTAGCACAAA
CAGATGAGCAAGACATGGGAATGACTTATGCTGAATTGAGCGAATTTGGAAGATTGAGAA
AACAAGCAGCATGTGGTCCATATTCTATGTTTTGTAAATTAGTGGCAACATGGCGTAATG
AATTTTCTCCAAAGGAAGTAAGTGACAAAGTTAAACATTTCTTCCGTTGTTATGCAATAA
ATCGTCATAAAATGACTGTTTTAACTCCTTCTGTACATATGGAAAGATATTCACCTGATG
ATAATCGATTTGATCATCGACCTTTCCTTTATCGAACTAATTGGAGTTGGCAATTTAAAG
CCATTGATAATGAGTTGGAAAGAATTATGCAAACTTCCACTAAAGGAGCACAAAAACATG
ACAGAAATGAACATGAAAATAAAGAAAAAGAAGAAATTTAA

>g17053.t5 Gene=g17053 Length=573
MIAIATNQLTVPFGDGVISTRETCIGFEICEELWNPRSTHIDLGLAGVEIIANGSSSYIQ
LRKAHITADLIKNATYKAGGAYLFSNLRGCDGQRVWWGGNSAIGLNGEIIAKSKQFSLLD
VEVTVATIDLEDIRSYRMALRSRCTFGASSPVYPRIAVDCELSSRRDVLIPVNLPLEWIN
HTPEEEIAMGPACWLWDYLRRSGQGGYFLPLSGGIDSCSTACIVYNMCQMVVDAIQNGED
DVLRDVRKITADPEFSPQTAAELCNKIFVTCFMGSKNSSKETKNRAAVLANEIGSYHLEL
NIDSVFDTILSVFKAVTGFIPKYRVQGGSTRENLALQNIQARVRMVLSYLFAQLVLWTRN
RPGGLLVLGSANVDESLRGYMTKYDCSSADVNPIGGISKTDLRRFLNYAMKKYNIPILNE
IFLAQPTAELEPLHEGQLAQTDEQDMGMTYAELSEFGRLRKQAACGPYSMFCKLVATWRN
EFSPKEVSDKVKHFFRCYAINRHKMTVLTPSVHMERYSPDDNRFDHRPFLYRTNWSWQFK
AIDNELERIMQTSTKGAQKHDRNEHENKEKEEI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g17053.t5 CDD cd00553 NAD_synthase 183 505 3.39816E-90
8 g17053.t5 Gene3D G3DSA:3.60.110.10 - 2 144 5.2E-35
7 g17053.t5 Gene3D G3DSA:3.40.50.620 HUPs 183 551 1.1E-138
12 g17053.t5 MobiDBLite mobidb-lite consensus disorder prediction 552 573 -
11 g17053.t5 MobiDBLite mobidb-lite consensus disorder prediction 554 573 -
3 g17053.t5 PANTHER PTHR23090:SF9 GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE 1 547 2.3E-245
4 g17053.t5 PANTHER PTHR23090 NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE 1 547 2.3E-245
9 g17053.t5 PIRSF PIRSF006630 NADS_GAT 2 539 1.1E-113
1 g17053.t5 Pfam PF00795 Carbon-nitrogen hydrolase 10 137 8.4E-15
2 g17053.t5 Pfam PF02540 NAD synthase 194 456 2.7E-26
14 g17053.t5 ProSiteProfiles PS50263 Carbon-nitrogen hydrolase domain profile. 1 130 17.847
6 g17053.t5 SUPERFAMILY SSF56317 Carbon-nitrogen hydrolase 15 142 5.49E-19
5 g17053.t5 SUPERFAMILY SSF52402 Adenine nucleotide alpha hydrolases-like 176 517 2.6E-62
13 g17053.t5 TIGRFAM TIGR00552 nadE: NAD+ synthetase 185 505 2.6E-24

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006807 nitrogen compound metabolic process BP
GO:0005524 ATP binding MF
GO:0004359 glutaminase activity MF
GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity MF
GO:0005737 cytoplasm CC
GO:0009435 NAD biosynthetic process BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed