General information for g17215.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Pyrimidodiazepine synthase.
Parent gene
- The parent gene g17215 is located on chr_4: 12845638..12851882 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g17215 | g17215.t1 | TSS | g17215.t1 | 12844546 | 12844546 |
| chr_4 | g17215 | g17215.t1 | isoform | g17215.t1 | 12845019 | 12846442 |
| chr_4 | g17215 | g17215.t1 | exon | g17215.t1.exon1 | 12845019 | 12845049 |
| chr_4 | g17215 | g17215.t1 | cds | g17215.t1.CDS1 | 12845019 | 12845049 |
| chr_4 | g17215 | g17215.t1 | exon | g17215.t1.exon2 | 12845543 | 12846067 |
| chr_4 | g17215 | g17215.t1 | cds | g17215.t1.CDS2 | 12845543 | 12846067 |
| chr_4 | g17215 | g17215.t1 | exon | g17215.t1.exon3 | 12846258 | 12846442 |
| chr_4 | g17215 | g17215.t1 | cds | g17215.t1.CDS3 | 12846258 | 12846442 |
| chr_4 | g17215 | g17215.t1 | TTS | g17215.t1 | 12846528 | 12846528 |
Sequences
>g17215.t1 Gene=g17215 Length=741
ATGACTATTAAAACACTTCATTTAACAAAAGGTTCATCCCAACCAACATTTCCTGACAAT
GAAATTCTTCGACTTTATTCAATGAAATATTGTCCTTATGCTCATCGAGCTCATTTGGTA
CTTGATGCAAAAAACATCCCTTATCACACTGTTTTTATTAATTTAACTGAGAAACCAGAA
TGGCTTTCCAAAGTTTCAGCACTCACAAAAGTTCCAGCTTTAGAATTACCAGGAGTTAAA
GGAGATTCTTTGATTGAATCTTTAATTATTTGTGACTACATTGATGAAAAATATCCACAA
AATTCTTTGAATTCTAAAGATCCATTAGAGAAAGCGCGTGACAGAATTTTAGTGCAAAAA
TTTGAAACTATATCACCAGTTTTTGCTCGTTTTGTATTTTGGAAGAAAGAAGAAGATAAA
GGTGAACTTTTGAAAAAACTTTATGCAGGATTGAAGAATTTTGATGACGAATTAAATGAA
AGAAATTCCAAATTCTTTGGTGGTGAAAAACCTGGCATGACTGATTATATGATTTGGCCT
TGGTTTGAAAGATTTGAAGTTTTAAATTTCATGTTGGAAGAAAAATTCAAATTGGACAAA
GATCGTTTTCCAAAATTGTCAGAATGGTTTGATGGAATGATGAATGATGAAGCTGTAAGA
AGAAATTTCATAGACATTGACGATCATTATAGATTTGTGACAACAAGAGATTATGACAAT
GCTGCTGAAAATAAATTGTAA
>g17215.t1 Gene=g17215 Length=246
MTIKTLHLTKGSSQPTFPDNEILRLYSMKYCPYAHRAHLVLDAKNIPYHTVFINLTEKPE
WLSKVSALTKVPALELPGVKGDSLIESLIICDYIDEKYPQNSLNSKDPLEKARDRILVQK
FETISPVFARFVFWKKEEDKGELLKKLYAGLKNFDDELNERNSKFFGGEKPGMTDYMIWP
WFERFEVLNFMLEEKFKLDKDRFPKLSEWFDGMMNDEAVRRNFIDIDDHYRFVTTRDYDN
AAENKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g17215.t1 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 7 | 235 | 0.000 |
| 9 | g17215.t1 | Gene3D | G3DSA:1.20.1050.10 | - | 104 | 224 | 0.000 |
| 3 | g17215.t1 | PANTHER | PTHR43968 | - | 6 | 235 | 0.000 |
| 6 | g17215.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 21 | 36 | 0.000 |
| 5 | g17215.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 98 | 112 | 0.000 |
| 4 | g17215.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 165 | 184 | 0.000 |
| 2 | g17215.t1 | Pfam | PF13417 | Glutathione S-transferase, N-terminal domain | 25 | 101 | 0.000 |
| 1 | g17215.t1 | Pfam | PF00043 | Glutathione S-transferase, C-terminal domain | 124 | 216 | 0.000 |
| 12 | g17215.t1 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 21 | 102 | 19.260 |
| 11 | g17215.t1 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 107 | 236 | 17.747 |
| 13 | g17215.t1 | SFLD | SFLDG00358 | Main (cytGST) | 23 | 215 | 0.000 |
| 14 | g17215.t1 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 23 | 215 | 0.000 |
| 7 | g17215.t1 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 24 | 122 | 0.000 |
| 8 | g17215.t1 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 106 | 235 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004364 | glutathione transferase activity | MF |
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
| GO:0005737 | cytoplasm | CC |
KEGG
Orthology
- This transcript has been annotated with K00799 (glutathione S-transferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
