Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 313b1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
HiC_scaffold_385 g17837 g17837.t1 isoform g17837.t1 33275 34897
HiC_scaffold_385 g17837 g17837.t1 exon g17837.t1.exon1 33275 33882
HiC_scaffold_385 g17837 g17837.t1 cds g17837.t1.CDS1 33275 33882
HiC_scaffold_385 g17837 g17837.t1 exon g17837.t1.exon2 34030 34897
HiC_scaffold_385 g17837 g17837.t1 cds g17837.t1.CDS2 34030 34897
HiC_scaffold_385 g17837 g17837.t1 TSS g17837.t1 NA NA
HiC_scaffold_385 g17837 g17837.t1 TTS g17837.t1 NA NA

Sequences

>g17837.t1 Gene=g17837 Length=1476
ATGTCGCTGATTTTAATAATTTTAGTAGTAACTTTAACTTCACTTTTTGCAATTTTTCGC
TTCAAACATCGAAGATTTTATAAATTGGCAGCGAAAATTCCAAAACTTCCTGGTGGACTT
CCAATAATTGGAGCAACTCATATTTTTATGACAGCTGAACCAAAGGATTATTTGCCAATA
ATGTTGGATATGATTAAAAAAGACATGGTAATTGGATCAGTTTGGTTTGGGACACAATTT
GCTGTCATTTTAAATACAGTTGAAGATTTTCAAAAAGTTTTAACTTCACCAAATTGCATC
AATAAACCAAAACTTCTTTTTGATGCTTTCTTTTCTGATTCTGCAAGTTTTTGTGTTAAT
AATAATCCACATGAAAAGAGAAGAAAACTTTTAAATCCATCATTTACAAGTTTGATGTTA
AATAAAACAAATTTTCATACAAATGAAACAATTAAAGGATTTATAAATAAAATAAATGCA
AAAAGTGATGGAAAAACTATTGATATTTATCACGACATTGCTGCATGTGCTTTGAGAGGG
ACACTTCATGCTCATTTTGATTATGATGATGATTCACTTGATTATAAAGTTGTTAAAGGA
TTTGATGACTTCAAACCTTTAATAATGCAACGCTTAGGCAAACCATGGCTTTCATTCAAG
CCATTTTTCAAACTATCAAAACTCTCAAAACAAGTTGAAGCTGTTAGAACACCAATTTAT
GAATGCATCAACAAAATCATCGAAAGCAACAAACATCATCGTATTAAACTTGAAGGTCGT
TGTGTGTCAACAATTCGACAACTTTTAGATACAAAACAAAAATTATCAGATGAAGAAATA
GCTGAAGAAATTTTTATTTTTATTTATGCATCACATGAAACTTCAGCTTTAGCTTTATCA
GCTTGTCTTTTGCTTCTTGCAATGCATAAAAATGTTGAAGAAAAAGTTTTTGAAGAAATT
AAAAATGCAAAATTAAGTGAAGAATTTACGTCCATTAGTAATGAAGAAACACAAAGTTTT
CCATACATTGAAATGGTTATAAAAGAAACAATGCGACTTTTCCCACCAGTTGCTGTGACT
TTTAGGGAAACAATTGCTGAAGTTGAAGTTGGTGATTATTTAATTCCACCTGATACAATT
GTTGGTTTGCCAGTTTTATTAATTCATAGAAATGAAAAAATTTGGGGAAAAGATGCTGGA
AGTTTCAGACCAGAACGTTTTGAACCAGAAGAATTTAAAAAAATTCCACAATTTGCATTT
TTACCTTTTCTGTTGGGAAAACGAACTTGCATTGGTTACAAATATGCAATGTATTTTATG
AAAATTTTTATTATAAATTTTGTAAAAAATTACAAAGTTGAAACAGATTTAAAATTTGAT
GAACTAAAAACTCAAATGACGCCAGCAACTTCACTTTTACAGGCTATCCAATTAAAATTA
TTAAAAGGAAGTGAAAATTTTTTAATAGGTCAATAA

>g17837.t1 Gene=g17837 Length=491
MSLILIILVVTLTSLFAIFRFKHRRFYKLAAKIPKLPGGLPIIGATHIFMTAEPKDYLPI
MLDMIKKDMVIGSVWFGTQFAVILNTVEDFQKVLTSPNCINKPKLLFDAFFSDSASFCVN
NNPHEKRRKLLNPSFTSLMLNKTNFHTNETIKGFINKINAKSDGKTIDIYHDIAACALRG
TLHAHFDYDDDSLDYKVVKGFDDFKPLIMQRLGKPWLSFKPFFKLSKLSKQVEAVRTPIY
ECINKIIESNKHHRIKLEGRCVSTIRQLLDTKQKLSDEEIAEEIFIFIYASHETSALALS
ACLLLLAMHKNVEEKVFEEIKNAKLSEEFTSISNEETQSFPYIEMVIKETMRLFPPVAVT
FRETIAEVEVGDYLIPPDTIVGLPVLLIHRNEKIWGKDAGSFRPERFEPEEFKKIPQFAF
LPFLLGKRTCIGYKYAMYFMKIFIINFVKNYKVETDLKFDELKTQMTPATSLLQAIQLKL
LKGSENFLIGQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g17837.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 17 482 1.3E-83
2 g17837.t1 PANTHER PTHR24291:SF145 CYTOCHROME P450 4AA1-RELATED 9 464 3.7E-65
3 g17837.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 9 464 3.7E-65
8 g17837.t1 PRINTS PR00385 P450 superfamily signature 290 307 3.0E-8
7 g17837.t1 PRINTS PR00463 E-class P450 group I signature 299 325 2.4E-7
6 g17837.t1 PRINTS PR00463 E-class P450 group I signature 344 362 2.4E-7
10 g17837.t1 PRINTS PR00385 P450 superfamily signature 345 356 3.0E-8
5 g17837.t1 PRINTS PR00463 E-class P450 group I signature 420 430 2.4E-7
9 g17837.t1 PRINTS PR00385 P450 superfamily signature 421 430 3.0E-8
4 g17837.t1 PRINTS PR00463 E-class P450 group I signature 430 453 2.4E-7
11 g17837.t1 PRINTS PR00385 P450 superfamily signature 430 441 3.0E-8
1 g17837.t1 Pfam PF00067 Cytochrome P450 37 470 2.6E-67
16 g17837.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
17 g17837.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
18 g17837.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 12 -
19 g17837.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 13 17 -
15 g17837.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 491 -
13 g17837.t1 SUPERFAMILY SSF48264 Cytochrome P450 37 462 1.11E-81
12 g17837.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed