Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 313b1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
HiC_scaffold_385 g17840 g17840.t1 isoform g17840.t1 38138 40035
HiC_scaffold_385 g17840 g17840.t1 exon g17840.t1.exon1 38138 38692
HiC_scaffold_385 g17840 g17840.t1 cds g17840.t1.CDS1 38138 38692
HiC_scaffold_385 g17840 g17840.t1 exon g17840.t1.exon2 38992 39253
HiC_scaffold_385 g17840 g17840.t1 cds g17840.t1.CDS2 38992 39253
HiC_scaffold_385 g17840 g17840.t1 exon g17840.t1.exon3 39437 40035
HiC_scaffold_385 g17840 g17840.t1 cds g17840.t1.CDS3 39437 40035
HiC_scaffold_385 g17840 g17840.t1 TSS g17840.t1 NA NA
HiC_scaffold_385 g17840 g17840.t1 TTS g17840.t1 NA NA

Sequences

>g17840.t1 Gene=g17840 Length=1416
ATGTTTTTAAGTCTCACATTTTTAGCTTCAATTTTTATCACAATTCAATTCTATAAATGG
ATCAAATTTTATCTAAAAATTTCTAAAATTCCTGGTCCAAAAAATCAAATTCCATTCTTT
GGTATAATCAATAATTTCATTGGAGAAAAAGTTGGCAATTATGGAAAAAATTTTTTAGAA
ATTATTAGTAAAACTGAACCAATCCAAAAAGTTTGGTTTGGTCCAAAAATTTTCATCATT
CCAAATACACCAGAAATGTTCAAGACAATTTTTACATCACTTTATTGTGTTGACAAACCA
AGATATATTTATGGTGGATTTTTTGCAAATAATGGCTTACTTTCAAGCAATGGCAGTGTA
CAGGAAAAACATAGAAAAATTCTAAGCTATTCAATGACTCCGAAAATGTTAAATCAACTT
GTGCCAGTTTTTGAAGAAAAAATTCAGAAATTTATAAAAAGAATGAAAAATAAAATTGAT
GGTGATGAATTTAATGTTTTTGAAGATGTTGCTGCATGTACTTTAGAAGCATTAGTGAAA
GGAAATTTTCAATATGATTATGACTGTTATGAAAGTGATTTGTTAAAAGCTTGTGCAAGT
TCAGATGGAATAACACTAAGAAGAATAATGCAACCTTGGATGTCATTTGAATTTTTATTT
AAAATTTCTCAACTTTCAAAAGATGTTAAGAGAATTTTTGGACCAATTTATTCAGCTGTT
GATAAAATTTTAGAACAAAATGAACATTTGAGAATTGAAAGTGATGACAAAAAAGGAATT
TTCATTAAACAATTATTGAATTTAAAAAATGAATTCAGTGAAGAAGAAATTCGAGATGAA
ATTTTTATTTTAATTTTTGCTGGTTCTGAAACCACAGCAACAACGCTTTCAATGTGTCTT
CTTCTTCTAGCAATGCACAAAAATATTCAAAACAAAATTATTGAGGAAATTAACACCAAA
AATTCAAATGATGACTTTCCCTACATTGAAATGGTTCTTAAAGAAGTTATGAGACTTTTT
CCAATTGGTCCAATAATTTTTAGAGAAACAACAGCTGAAATAATGTTGGAAAGTTGTGAA
GTACCAAAAAACACTACAATAGCAATTCCAATTTATTTTCTTCATAGAAATGAAAATATT
TGGGGAAAAGATGCAAATGAATTTAAGCCAGAACGTTTTGAAGCTAATAAAATTGACAAA
ATTCATTCATATTCTTTTGTTCCATTCTCAGGTGGTAAAAGAATGTGCATTGGTTACAAA
TATGCAATGACTTTTATGAAAATTTTTATTGTAAATTTTTTCAAAGAATTTGAAATTGAA
ACAAGTTTAAAATATGAAGAAATGGATGTCAAAGTTCTTCCAACAATTACTGTAACTCAA
GGTTTTCCTATTAAAATAAAGAAACGAACAATTTGA

>g17840.t1 Gene=g17840 Length=471
MFLSLTFLASIFITIQFYKWIKFYLKISKIPGPKNQIPFFGIINNFIGEKVGNYGKNFLE
IISKTEPIQKVWFGPKIFIIPNTPEMFKTIFTSLYCVDKPRYIYGGFFANNGLLSSNGSV
QEKHRKILSYSMTPKMLNQLVPVFEEKIQKFIKRMKNKIDGDEFNVFEDVAACTLEALVK
GNFQYDYDCYESDLLKACASSDGITLRRIMQPWMSFEFLFKISQLSKDVKRIFGPIYSAV
DKILEQNEHLRIESDDKKGIFIKQLLNLKNEFSEEEIRDEIFILIFAGSETTATTLSMCL
LLLAMHKNIQNKIIEEINTKNSNDDFPYIEMVLKEVMRLFPIGPIIFRETTAEIMLESCE
VPKNTTIAIPIYFLHRNENIWGKDANEFKPERFEANKIDKIHSYSFVPFSGGKRMCIGYK
YAMTFMKIFIVNFFKEFEIETSLKYEEMDVKVLPTITVTQGFPIKIKKRTI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g17840.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 15 471 1.4E-93
2 g17840.t1 PANTHER PTHR24291:SF140 CYTOCHROME P450 4C3-RELATED 5 469 1.0E-74
3 g17840.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 5 469 1.0E-74
8 g17840.t1 PRINTS PR00463 E-class P450 group I signature 276 293 2.4E-14
12 g17840.t1 PRINTS PR00385 P450 superfamily signature 287 304 7.1E-13
7 g17840.t1 PRINTS PR00463 E-class P450 group I signature 296 322 2.4E-14
5 g17840.t1 PRINTS PR00463 E-class P450 group I signature 330 348 2.4E-14
11 g17840.t1 PRINTS PR00385 P450 superfamily signature 331 342 7.1E-13
6 g17840.t1 PRINTS PR00463 E-class P450 group I signature 406 416 2.4E-14
9 g17840.t1 PRINTS PR00385 P450 superfamily signature 407 416 7.1E-13
4 g17840.t1 PRINTS PR00463 E-class P450 group I signature 416 439 2.4E-14
10 g17840.t1 PRINTS PR00385 P450 superfamily signature 416 427 7.1E-13
1 g17840.t1 Pfam PF00067 Cytochrome P450 31 442 3.8E-74
18 g17840.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
19 g17840.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
20 g17840.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
22 g17840.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 17 -
17 g17840.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 280 -
21 g17840.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 281 304 -
16 g17840.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 305 471 -
14 g17840.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 409 418 -
13 g17840.t1 SUPERFAMILY SSF48264 Cytochrome P450 30 469 4.98E-94

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed