Gene loci information

Transcript annotation

  • This transcript has been annotated as V-type proton ATPase 116 kDa subunit a isoform 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1818 g1818.t1 TTS g1818.t1 13167308 13167308
chr_3 g1818 g1818.t1 isoform g1818.t1 13167532 13169795
chr_3 g1818 g1818.t1 exon g1818.t1.exon1 13167532 13167806
chr_3 g1818 g1818.t1 cds g1818.t1.CDS1 13167532 13167806
chr_3 g1818 g1818.t1 exon g1818.t1.exon2 13167865 13168713
chr_3 g1818 g1818.t1 cds g1818.t1.CDS2 13167865 13168713
chr_3 g1818 g1818.t1 exon g1818.t1.exon3 13168779 13168789
chr_3 g1818 g1818.t1 cds g1818.t1.CDS3 13168779 13168789
chr_3 g1818 g1818.t1 exon g1818.t1.exon4 13168960 13169699
chr_3 g1818 g1818.t1 cds g1818.t1.CDS4 13168960 13169699
chr_3 g1818 g1818.t1 exon g1818.t1.exon5 13169763 13169795
chr_3 g1818 g1818.t1 cds g1818.t1.CDS5 13169763 13169795
chr_3 g1818 g1818.t1 TSS g1818.t1 NA NA

Sequences

>g1818.t1 Gene=g1818 Length=1908
ATGATTGAAGCTCCATTAGAGGATCCATCTAACGGCGATAATGTTTACAAATCTGTATTC
ATTATTTTCTTCCAAGGAGATCAGTTGAAAACTCGTGTTAAGAAAATTTGTGAAGGTTTT
CGTGCAACATTATATCCATGCCCAGAAGCTCCTGCTGATCGTCGTGAAATGGCAATGGGT
GTCATGACAAGGATCGAAGATTTGAATACTGTTTTAGGACAAACTCAAGACCATCGTCAT
CGTGTGCTTGTTGCTGCAGCAAAAAATTTGAAAAATTGGTTTGTTAAAGTGCGTAAGATA
AAAGCTATTTATCATACGCTTAATCTTTTCAATTTGGATGTCACTCAAAAATGTTTGATC
GCTGAATGCTGGGTTCCTGTTCTTGATTTAGAGAGCATTCAAATGGCATTGCGAAGAGGA
ACTGAAAGAAGTGGCGGCTCTTCAGTTCCACCAATTTTGAATCGCATGGAGACTTATGAA
GATCCTCCTACTTACAATCGAACAAATAAGTTTACTAAAGCTTTTCAAGCTCTGATTGAT
GCATATGGTGTAGCAAGTTATCGTGAAATGAATCCTGCACCATATACTATCATTACATTC
CCATTTTTGTTCGCTGTGATGTTTGGTGATTTGGGTCACGGAACTATAATGGCTCTCTTT
GGATTATGGATGATTTTGAAAGAAAAACCATTATCGGCAAAAAAATCTGATAATGAGATT
TGGAACATTTTCTTCGGTGGTCGTTATATTATATTCTTGATGGGTGTTTTTAGTATGTAC
ACAGGTTTAATTTACAATGATATATTCTCAAAATCACTCAATATCTTTGGATCTGGATGG
ACAATTAATTATAATGAAAGTACGATTATGACAAATAAAGCATTGCAATTAGATCCACGT
GACGCTTTTCATCGTACTTATCCACTTGGTATGGACCCAGTATGGCAGGTTGCACCATTA
AACAAAATTATCTTCATGAATGGATTTAAGATGAAATTGAGCATTATTTTGGGTGTTATT
CATATGATTTTTGGCGTTGTAGTTGGCTTATTTAATCATCGTTACTTCAAAAATACTCTC
AACATTTATGCTGAGTTTATTCCACAGATGATCTTCTTGATATTCTTATTTTTCTACATG
ACTCTCTTAATGTTTATCAAATGGATGCGATACTCGGCATCAAATCCCGATCCAAAATCA
TCTCATTGCGCACCTTCAATTTTAATTACATTTATCAATATGGTGTTGTTTAAAGAATCG
ACTGTATTAACAGAATCTTGTGAAACACCTTATATGTATTCAGGACAAGCAGGAATTCAA
AGACTTTTAGTTCTCAGTGCATTTGTCTGTGTTCCGTGGATGCTTTTTGCAAAACCAATA
ATTATTATGCAAGAGCGTAAAAAGCGACAACATCAACCTATTTTACCATATAGCGGTGAA
GGTCAAGATGCCGAAAATGGGCAACCGCAATCAATTCAACCAGCTGTAACTGAAAGTCAT
GGAGGACATGATAATGAGGATATGAGTGAAATTTTCATTCATCAAGGAATACATACAATT
GAATATGTACTGGGTTCAGTCAGTCATACAGCATCATATCTTCGTTTATGGGCTCTCTCG
TTGGCACATGCACAACTTGCTGAAGTTTTATGGAACATGGTTTTACAAAATGGTTTAAAA
ACTGGTGGAGCTATAGGAGGTGTTGCATTATGGGCAATTTTTGCTTTTTGGGCAATTCTC
ACTGTTGGCATTTTGGTTCTAATGGAAGGACTTTCAGCTTTTCTGCATACACTTCGTCTT
CATTGGGTCGAATTTCAATCCAAGTTTTATGCTGGACAAGGATACGCTTTTCAACCATTT
GCTTTTGAAGCTATTTTGGAAGGAAGTGGTAGTTCAACTGAAGAGTAA

>g1818.t1 Gene=g1818 Length=635
MIEAPLEDPSNGDNVYKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMG
VMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLI
AECWVPVLDLESIQMALRRGTERSGGSSVPPILNRMETYEDPPTYNRTNKFTKAFQALID
AYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGTIMALFGLWMILKEKPLSAKKSDNEI
WNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSGWTINYNESTIMTNKALQLDPR
DAFHRTYPLGMDPVWQVAPLNKIIFMNGFKMKLSIILGVIHMIFGVVVGLFNHRYFKNTL
NIYAEFIPQMIFLIFLFFYMTLLMFIKWMRYSASNPDPKSSHCAPSILITFINMVLFKES
TVLTESCETPYMYSGQAGIQRLLVLSAFVCVPWMLFAKPIIIMQERKKRQHQPILPYSGE
GQDAENGQPQSIQPAVTESHGGHDNEDMSEIFIHQGIHTIEYVLGSVSHTASYLRLWALS
LAHAQLAEVLWNMVLQNGLKTGGAIGGVALWAIFAFWAILTVGILVLMEGLSAFLHTLRL
HWVEFQSKFYAGQGYAFQPFAFEAILEGSGSSTEE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g1818.t1 PANTHER PTHR11629 VACUOLAR PROTON ATPASES 2 628 3.3E-297
3 g1818.t1 PANTHER PTHR11629:SF61 V-TYPE PROTON ATPASE SUBUNIT A 2 628 3.3E-297
1 g1818.t1 Pfam PF01496 V-type ATPase 116kDa subunit family 2 622 6.0E-273
12 g1818.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 196 -
22 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 197 226 -
15 g1818.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 227 245 -
25 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 246 265 -
11 g1818.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 266 332 -
21 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 333 351 -
17 g1818.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 352 370 -
24 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 371 389 -
13 g1818.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 390 400 -
23 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 401 418 -
16 g1818.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 419 437 -
20 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 438 461 -
14 g1818.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 462 535 -
19 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 536 556 -
18 g1818.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 557 567 -
26 g1818.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 568 588 -
10 g1818.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 589 635 -
9 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 201 223 -
4 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 244 266 -
7 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 333 351 -
8 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 364 386 -
5 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 439 461 -
6 g1818.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 565 587 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0033179 proton-transporting V-type ATPase, V0 domain CC
GO:1902600 proton transmembrane transport BP
GO:0015078 proton transmembrane transporter activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values