Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Translation initiation factor eIF-2B subunit alpha.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1977 g1977.t4 TTS g1977.t4 14167622 14167622
chr_3 g1977 g1977.t4 isoform g1977.t4 14167742 14168784
chr_3 g1977 g1977.t4 exon g1977.t4.exon1 14167742 14168281
chr_3 g1977 g1977.t4 cds g1977.t4.CDS1 14167742 14168191
chr_3 g1977 g1977.t4 exon g1977.t4.exon2 14168344 14168607
chr_3 g1977 g1977.t4 exon g1977.t4.exon3 14168670 14168784
chr_3 g1977 g1977.t4 TSS g1977.t4 14168843 14168843

Sequences

>g1977.t4 Gene=g1977 Length=919
ATGGATAATGCTCAAATCGAAAGATATTTTCTTAATATCGTTAATAACGACAATAATATG
TCGTATGGAATAGCAGCAATTAAAACTTTACTTATGGTTCTCGAGAAAACAACTTTTGTG
ACAATTCAAGAGTTGATTAATACAATAAAATCTGCAATTAAAGTAATAAAAGAAAGTGGA
AAACCAATAACATCAATTAGTTCTTCATGTGAACTTTTCATGTGCTTCATCACTATCTCA
AGTCCAAAAATTGATGAAATGTATGACATGGAAGAAGTAAAGAAAATAATGACTAATAGA
GGACAAAGTTTCTTAAGAAAACTGCTGGCCAGTCGTGAAGTTATTGCTAAAAATTCTGTA
GACTTTATTACAGATGGATAGAATCTTGACAATAGGTCGATCAAGAGTAGTGTATGAAGC
ATTAAAACTAGCTGCCTCAAAGAACAAACATTTTCGAGTATATGTTACAATGAGCATGAG
TTCCTTTAAAAATGAAAATGAGGGAGAGATGATGGTACAGGATTTAAAGCAATTAAACAT
TGATGCTTCATTAGTGCTTGATGCTGCAATTGGATACACAATGGAAAGCATTGACTTTGT
TTTTTCTGGTGCAGAAGGCGTAATGGAAAATGGTGGGATAATAAATAGAATAGGAACCTT
TACTGCTGCATTGTGTGCCAGTGAAATGAACAAACCATTTTACGTCTTAACTGAAAGTTT
CAAATTCTCTCGAATTTATCCACTCAATCAACGAGATATACCGAGTACTTATAAATTTAT
ACAAAACAGAAATGGCGAAAAGGCAAATATTCATCCGTTAGTTGACTTTACACCTCCACT
CTTTATAACTCTCATATTTACTGATCTTGGAATTTTGACACCTTCTGCTGTTAGTGATGA
ACTTATAAAACTTTATTAA

>g1977.t4 Gene=g1977 Length=149
MSMSSFKNENEGEMMVQDLKQLNIDASLVLDAAIGYTMESIDFVFSGAEGVMENGGIINR
IGTFTAALCASEMNKPFYVLTESFKFSRIYPLNQRDIPSTYKFIQNRNGEKANIHPLVDF
TPPLFITLIFTDLGILTPSAVSDELIKLY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g1977.t4 Gene3D G3DSA:3.40.50.10470 - 4 149 0
2 g1977.t4 PANTHER PTHR45860:SF1 TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA 8 149 0
3 g1977.t4 PANTHER PTHR45860 TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA 8 149 0
1 g1977.t4 Pfam PF01008 Initiation factor 2 subunit family 10 138 0
4 g1977.t4 SUPERFAMILY SSF100950 NagB/RpiA/CoA transferase-like 10 149 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0044237 cellular metabolic process BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values