General information for g2031.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as WASH complex subunit 4.
Parent gene
- The parent gene g2031 is located on chr_3: 14589657..14592786 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2031 | g2031.t1 | TSS | g2031.t1 | 14589258 | 14589258 |
| chr_3 | g2031 | g2031.t1 | isoform | g2031.t1 | 14589335 | 14592786 |
| chr_3 | g2031 | g2031.t1 | exon | g2031.t1.exon1 | 14589335 | 14590456 |
| chr_3 | g2031 | g2031.t1 | cds | g2031.t1.CDS1 | 14589335 | 14590456 |
| chr_3 | g2031 | g2031.t1 | exon | g2031.t1.exon2 | 14590521 | 14592281 |
| chr_3 | g2031 | g2031.t1 | cds | g2031.t1.CDS2 | 14590521 | 14592281 |
| chr_3 | g2031 | g2031.t1 | exon | g2031.t1.exon3 | 14592340 | 14592786 |
| chr_3 | g2031 | g2031.t1 | cds | g2031.t1.CDS3 | 14592340 | 14592786 |
| chr_3 | g2031 | g2031.t1 | TTS | g2031.t1 | 14592888 | 14592888 |
Sequences
>g2031.t1 Gene=g2031 Length=3330
ATGAATTTTCAAAGTAATGCAGATAATAAAGCTGCTGACGATCTGTTAAAAAAATTTTCA
AAATTCATCAGATCATATAAAGAAACGACTGGAAAGTTGATAAACGAAAAATCTTACAAT
GAAAAATTTTTTCAACTCAAGTCTGATCCAAATTCCGAAAATGTGTCAGTCTTTCAATTA
CTTGAGTCTGAAAATAAACTGCTCACTAAATGTATGATTGTATTTTATCATCTCAACAAA
GAGGCTAGAAAGTTAATCAAACATTCCAAAATAATTGCAAATAAGCTGATTGTAATTGAA
GATGAAATCATCAGTGTAAGTGAGGCATCAACTGAAGCTAGTGAAGAAAATGCTTTAGTA
AAATTTAGTTACTCATTAGAGGATCTCTTTGACATGAAATTTCTTGTTCAAAACGCCATC
TTCTTATCAGTCAATGTGTTCCAGCAATATTGTGCGCTCTTTTCTATGGAAAAATTGCTT
CAAATCACACCATCATCAATTTTTCCATCAAATATTGATGATGTGTTATTATTACTGAGA
AATCTTGTGATCTTTGACATGATTTTTCAAAATAGACTTTTTAAAAATTATTTGGGTCTC
TATAGTGATCTCTTACTACAACAACAAAGTGATGACGAAAGGCATATCAATTTACAAAAT
ACCATTCATGAGATTGACTTATTAATTGATGGAAATATTTTCAATATAGCACTTGACAAT
ATAGTTGCGGTCAAGAACAAAATAAAGGCAAGGAATTTAAAACAATTAGAAAATTTTATG
TTGACACATATTAAGAATTTGATATATGGTATAAATATTCTTGGCAATAATCTCTCTGAA
TTGAATGACATTGACGAGTTGATGAAACTTAATATTGCCGTTATCGTCTATCAAAGGATT
TTTCAGAATTTAGATCAAAAACATATGAGACTTGTATCTGACATAAATTATAAATATCCC
GCAATTGTAATTCATGGTAATATCATGTTAAATGGAAATGAATTCCTTAAACGTTATCTG
CAACTTGAGAAGAACAATGTTGACATTGAGAAACTTCAGCAAAGTTATCTGAAACAAAGG
GTCGATGAAATTAATAAAGATACGTTGCAATATTCTAATCAAGTATTTTTATGGGTACTA
CAACTTCATCAACAATCAAAATTTGGTAATGAAAATTTAAATGAAGAGATGCTGAAAAAT
AGATGTGAATTATATTATCAAGGTTTAACACTTGCTGGAGAAATCAGTTTTATTATCAAA
ACCATCGTCAATTTGCACGCAAAACTTGGTCAACTTATATCAAAACCACTTGTTTCTAAC
ATTTATAAATTGACAGAAAATTTGCAGTTGATAAAATCTACTTATGAAGAAAAATCAAAA
TTCATCAGTGATTCGATCTCTTTTGTATTTCGATATCTGCAATTCCATACATTAAATGTG
ATTGGCTTGTGCAAAAAGAAAGTAATTATCGATGCAAAAAAGGAGCGAAAATTTGATCTT
TCAACAACACTTGTACTAATTGAGAAGTTGCTATATGCAAGCATGTCAATCAATAATTTG
AGTGCCATCACACTTGCATTGAATTTAACAGAGCCGTTTAAAAACTTTGGCCATGAATAT
TCAATAAGATTGATAAAACTTTTAGATCACATGATGACACTTTGTCGATTAGAGGAAAAT
TTAGAGAAAGTATGCGACACATCATTCCTGTTCTGGCATCAAAACTTATGTCAAACTTTT
TTCAAACAACATATGGAGACAACACTCGATGAACGAAAAATAAATTTGATAGTGCAATCG
ATTGAAGAGTGCATGAAAAATTGCAACTATCAAAAACTCGAACTTGATAATGTGCAAAAT
TTTTATCCATTCATAGAAAATCATTTTAGTGAGAATGTAACGAAGAAGCTTTGCAATTCG
ATTGAGATAAATTTACGTTTAGATTTTCATTCAAATTTACAAGTGGAAAAGTTTAATCCA
TTTGATGCTGCTTGTAAAATTTTAATGGAAGATCGAAGAGCGATGGTGAAATTAAATCCA
GCAGTTATACATGATGAATACATTTCAATACAAGACGATGTCGAATTCTATTTGAGTAAA
ATGTTTTACAATCTCACAACGATTTCTTTAAAAGATTGGCGAACTTATGAAGAAATGCGA
CGATTAGCACAAAGAAAATATTATTTGGAAACTGTTGAAGATCATTTACCAACTCAGACA
CTAGAGCAGGGCATAGATGTTTTACAAATTATGAGAAATATTCATTTATTTGTATCACGT
TTCAATTACAATCTAAATAATCAAATATTTATTGAAGCTATTGCTAATAATAATCAACAT
TTTAATACAGTCAGAATCAATAATATTGCTAATTCTCTGTCAACACATGGTTTCGGTATC
ATCAACACCACCATCAATTTTGTTTATCAATTTTTACGTAAAAAGCTTTCAATCTTTTCT
CAAGTTGTCTACGACGAAAGAATAAAATCGCGATTGACACGTGAGATGAAAATTTTTCGA
GACAACACTGCAGCAGCTGACAAGAATAGTGAAGTTTATAGTTTTGAGCGTGCTCTTAAT
TTACATGACTACATTAAAAATCTTGAAAGCTTTGAGGGAAATCAAACATTAATGGATAAA
ATAAGATCACTGATTTCATCAATTGGAAATTCTTTAGGATATATTCGTAGCGTTAGATCA
GGCATTAATCAAGTAAATTCCAATTCAATTTCTTTTGTACCTAATTTATTCAGTGATGAG
GAGTTGGAATTTGTAAAAATGTGTAATGGTTTAAGTCTAAATACAGTAAGAGCTTCTCAA
TGTCTAGAAAAGGATATTGGTCAAGGAATGAAAAAAATTGTTGAAGGAACACTTTATTTT
AAGATGCTAATGTCAACATTTTCACCCTACTTTCAAAGTTCGAAAAATTCACATTTGAAA
AATTTTTATCTTCTGGTTCCTGCTCTCACAATTAATTATGTCAAGTATATTCTCAAGTCA
AAAGATCGATTAAAGAAAACTCAAACTCAAGATGAATTGGTGTTTACTGATGATGGTTTT
AGTATCGGTATAGTTTATATTCTTCGCCTGTTAGACCAGCTTTCAGATTATACTTCATTA
AATTGGACCAAAACTATACGTGCTAAAATTAAAGGAGACAAAGACAATATAGTGAAGCAA
CGTGAAACTACGGCAAAAACTAACAAGAATAATGATGAGAAATTGTTGCAAACATTGAGT
TTGAGCGAGATGAGAATAAATGCTTTTCAAACTGAATTTGAATTACTCTTTTACAATATA
AATAGTGTGAAAATCTTCTTTCAAAGTTGA
>g2031.t1 Gene=g2031 Length=1109
MNFQSNADNKAADDLLKKFSKFIRSYKETTGKLINEKSYNEKFFQLKSDPNSENVSVFQL
LESENKLLTKCMIVFYHLNKEARKLIKHSKIIANKLIVIEDEIISVSEASTEASEENALV
KFSYSLEDLFDMKFLVQNAIFLSVNVFQQYCALFSMEKLLQITPSSIFPSNIDDVLLLLR
NLVIFDMIFQNRLFKNYLGLYSDLLLQQQSDDERHINLQNTIHEIDLLIDGNIFNIALDN
IVAVKNKIKARNLKQLENFMLTHIKNLIYGINILGNNLSELNDIDELMKLNIAVIVYQRI
FQNLDQKHMRLVSDINYKYPAIVIHGNIMLNGNEFLKRYLQLEKNNVDIEKLQQSYLKQR
VDEINKDTLQYSNQVFLWVLQLHQQSKFGNENLNEEMLKNRCELYYQGLTLAGEISFIIK
TIVNLHAKLGQLISKPLVSNIYKLTENLQLIKSTYEEKSKFISDSISFVFRYLQFHTLNV
IGLCKKKVIIDAKKERKFDLSTTLVLIEKLLYASMSINNLSAITLALNLTEPFKNFGHEY
SIRLIKLLDHMMTLCRLEENLEKVCDTSFLFWHQNLCQTFFKQHMETTLDERKINLIVQS
IEECMKNCNYQKLELDNVQNFYPFIENHFSENVTKKLCNSIEINLRLDFHSNLQVEKFNP
FDAACKILMEDRRAMVKLNPAVIHDEYISIQDDVEFYLSKMFYNLTTISLKDWRTYEEMR
RLAQRKYYLETVEDHLPTQTLEQGIDVLQIMRNIHLFVSRFNYNLNNQIFIEAIANNNQH
FNTVRINNIANSLSTHGFGIINTTINFVYQFLRKKLSIFSQVVYDERIKSRLTREMKIFR
DNTAAADKNSEVYSFERALNLHDYIKNLESFEGNQTLMDKIRSLISSIGNSLGYIRSVRS
GINQVNSNSISFVPNLFSDEELEFVKMCNGLSLNTVRASQCLEKDIGQGMKKIVEGTLYF
KMLMSTFSPYFQSSKNSHLKNFYLLVPALTINYVKYILKSKDRLKKTQTQDELVFTDDGF
SIGIVYILRLLDQLSDYTSLNWTKTIRAKIKGDKDNIVKQRETTAKTNKNNDEKLLQTLS
LSEMRINAFQTEFELLFYNINSVKIFFQS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g2031.t1 | PANTHER | PTHR31409 | WASH COMPLEX SUBUNIT 4 | 13 | 1108 | 1.9E-248 |
| 1 | g2031.t1 | Pfam | PF14745 | WASH complex subunit 7, N-terminal | 33 | 568 | 1.1E-60 |
| 3 | g2031.t1 | Pfam | PF14744 | WASH complex subunit 7 | 569 | 916 | 1.0E-111 |
| 2 | g2031.t1 | Pfam | PF14746 | WASH complex subunit 7, C-terminal | 935 | 1108 | 1.3E-53 |
| 6 | g2031.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 981 | - |
| 7 | g2031.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 982 | 998 | - |
| 5 | g2031.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 999 | 1109 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0071203 | WASH complex | CC |
KEGG
Orthology
- This transcript has been annotated with K18465 (WASH complex subunit 7)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
