General information for g2097.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein O-mannosyl-transferase 2.
Parent gene
- The parent gene g2097 is located on chr_3: 15197036..15199248 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2097 | g2097.t1 | isoform | g2097.t1 | 15191404 | 15197036 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon1 | 15191404 | 15191563 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS1 | 15191404 | 15191563 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon2 | 15193510 | 15193798 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS2 | 15193510 | 15193798 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon3 | 15193859 | 15194048 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS3 | 15193859 | 15194048 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon4 | 15194101 | 15194209 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS4 | 15194101 | 15194209 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon5 | 15194267 | 15195048 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS5 | 15194267 | 15195048 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon6 | 15195224 | 15195464 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS6 | 15195224 | 15195464 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon7 | 15196819 | 15196967 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS7 | 15196819 | 15196967 |
| chr_3 | g2097 | g2097.t1 | exon | g2097.t1.exon8 | 15197019 | 15197036 |
| chr_3 | g2097 | g2097.t1 | cds | g2097.t1.CDS8 | 15197019 | 15197036 |
| chr_3 | g2097 | g2097.t1 | TSS | g2097.t1 | NA | NA |
| chr_3 | g2097 | g2097.t1 | TTS | g2097.t1 | NA | NA |
Sequences
>g2097.t1 Gene=g2097 Length=1938
ATGGATAGTTGCATTGAGATGGTGCACGATGAATTGCAAGATTTTGCAACGCCATATTCG
ATCGTAAATCGTAATAATGATAATGAATATGTGTATGATAATGAACAATTTAATAATGAG
TCGAGTGATTCTGATGATTGCGAAATGGATTATAATTATGTTAAATTTTATGACTTTAAA
ATTCTTCTTCAAAATTACTTTTTATCAAGTTTTTTAATTCCACCTGTCACATCATTTTCA
AAAACATTTTGCATCATTTCACTCAAAGAAAGAGTTCAATGCAAGAACAAGAAGATGACG
TCGACGAAGAATAAAGAATCTTCAATTAAAAATTCACCCAAGAAAGTTGTCAATAATGAA
ACCAATTGGTATTTTACAGTCTTCGTTTTAGTCATTGCAGTCACATTTGCCACAAGATTT
TATAAAGTCACTGAGCCTGATCATGTTTGCTGGGATGAGACACACTTTGGAAAAATGGCA
AGTTGGTATATCAATCGAACTTTCTTTTTTGATGTTCATCCACCACTTGCAAAAATGCTA
ATAGCATTGTCAGGAAAATTAACGGGATATAATGGAACTTATGCTTTTGAAAAACCGTCT
GATAAATATAATGGAACAAAATATGAAGGAATGAGAATTTTCTGCACATTTTTGGGTGCA
ACAATAGTTCCAATGGCTTATGAAACTGTTAATGATATGACTGGATCTGTTTTAGCTGCT
TTTATTGCAGCTGTTTATCTTATTTTTGATGTCGGAATGATAACACTCAATCAATATATT
CTTCTTGATCCTCCTTTGCTATGTTTTATGACAGCAAGTTTAATGGGAATGGTTAAAGTA
TCAAAATATACAACAAGAAATCAAAGTTTCACAGTCAGATGGTGGCAATGGCTTACATTT
ACAGGAACGATGATTGCATGTACAATTAGTGTCAAATTCGTTGGACTTTTTATTGTTTTA
CTTGTTGGTCTTCAAACAATTAGTGACTTGTGGTTTGTTCTTGGTGATTTATCCAAGCCA
ATAATTTATTCCATCAAAGAATTCATCGCTCGTGCTGTGATGTTAATTGTTTTGCCAATT
TTTCTTTATGTATTTTTTTTCTACATTCATTTGAGAGTTCTCAATCATAGTGGAAATGGT
GATGGCTTTTATAGTTCAGGATTTCAAAGTCGATTGATTGGAAATTCACTTTATAACGCC
ACAATGCCTCGTGATGTTGCATACGGTGCAATTGTGACACTGAAAAATCATAAAACTGGT
GGTGGCTATCTTCATTCACACTATCATCTTTATCCTAAGAATTTTGGTGCAAGACAACAA
CAAATTACGACTTATACACATAAAGATGACAATAATAAATGGATAATTAAACCTTATGAC
AGAGAATCTTATGGAAAGAAAAATGTTACAATTGTTAAACATGGAGATTTAGTTAGACTT
GAACATTTAATGACAAGAAGAAATCTACATTCACATTCTGAAAAAGCACCAATTACTGTG
AAACATTTTCAAGTAACTGGATATGGAGAGAATGGCACAGGCGATGCAAATGACATTTTT
AAAATTCTCATTCTTAATGGAAAAGAAAGTGACTCAATACATACAGTGAGCACAAAATTT
AAGCTCGTTCATTATTTTCAACATTGTGCAGTGACATCGTCTGGCAAGCAGCTTCCAAAA
TGGGGCTTCGAGCAACAAGAAGTATCATGCAATCCTAACTTAAGAGACAAAAATGCATTG
TGGAATGTTGAAGATAATTATTTTGAATTATTGCCAAATGTGAGCTTTCACGTTTATGCT
CCGGGATTTTTCGCTCGTTTTCTCGAATCGCATGCGGTTATGCTGCAAGGAAATGCTGGA
TTGAAGCCAAAAGAGGGTGAAGTGACCAGCAGACCATGGCAATGGCCAATTAACTACAAG
AAAAATAAAATTAATTGA
>g2097.t1 Gene=g2097 Length=645
MDSCIEMVHDELQDFATPYSIVNRNNDNEYVYDNEQFNNESSDSDDCEMDYNYVKFYDFK
ILLQNYFLSSFLIPPVTSFSKTFCIISLKERVQCKNKKMTSTKNKESSIKNSPKKVVNNE
TNWYFTVFVLVIAVTFATRFYKVTEPDHVCWDETHFGKMASWYINRTFFFDVHPPLAKML
IALSGKLTGYNGTYAFEKPSDKYNGTKYEGMRIFCTFLGATIVPMAYETVNDMTGSVLAA
FIAAVYLIFDVGMITLNQYILLDPPLLCFMTASLMGMVKVSKYTTRNQSFTVRWWQWLTF
TGTMIACTISVKFVGLFIVLLVGLQTISDLWFVLGDLSKPIIYSIKEFIARAVMLIVLPI
FLYVFFFYIHLRVLNHSGNGDGFYSSGFQSRLIGNSLYNATMPRDVAYGAIVTLKNHKTG
GGYLHSHYHLYPKNFGARQQQITTYTHKDDNNKWIIKPYDRESYGKKNVTIVKHGDLVRL
EHLMTRRNLHSHSEKAPITVKHFQVTGYGENGTGDANDIFKILILNGKESDSIHTVSTKF
KLVHYFQHCAVTSSGKQLPKWGFEQQEVSCNPNLRDKNALWNVEDNYFELLPNVSFHVYA
PGFFARFLESHAVMLQGNAGLKPKEGEVTSRPWQWPINYKKNKIN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2097.t1 | Gene3D | G3DSA:2.80.10.50 | - | 395 | 590 | 2.9E-61 |
| 3 | g2097.t1 | PANTHER | PTHR10050 | DOLICHYL-PHOSPHATE-MANNOSE–PROTEIN MANNOSYLTRANSFERASE | 126 | 640 | 4.4E-239 |
| 4 | g2097.t1 | PANTHER | PTHR10050:SF46 | PROTEIN O-MANNOSYL-TRANSFERASE 2 | 126 | 640 | 4.4E-239 |
| 2 | g2097.t1 | Pfam | PF02366 | Dolichyl-phosphate-mannose-protein mannosyltransferase | 131 | 375 | 4.1E-72 |
| 1 | g2097.t1 | Pfam | PF02815 | MIR domain | 423 | 581 | 2.0E-30 |
| 13 | g2097.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 122 | - |
| 17 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 141 | - |
| 7 | g2097.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 142 | 209 | - |
| 14 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 210 | 227 | - |
| 10 | g2097.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 228 | 232 | - |
| 18 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 233 | 252 | - |
| 9 | g2097.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 253 | 258 | - |
| 16 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 259 | 278 | - |
| 12 | g2097.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 279 | 297 | - |
| 19 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 298 | 327 | - |
| 8 | g2097.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 328 | 347 | - |
| 15 | g2097.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 348 | 369 | - |
| 11 | g2097.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 370 | 645 | - |
| 30 | g2097.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 403 | 459 | 13.179 |
| 31 | g2097.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 469 | 525 | 11.637 |
| 29 | g2097.t1 | ProSiteProfiles | PS50919 | MIR domain profile. | 530 | 586 | 10.364 |
| 26 | g2097.t1 | SMART | SM00472 | mir_2 | 403 | 459 | 2.7E-11 |
| 27 | g2097.t1 | SMART | SM00472 | mir_2 | 469 | 525 | 2.1E-12 |
| 28 | g2097.t1 | SMART | SM00472 | mir_2 | 531 | 586 | 4.4E-10 |
| 5 | g2097.t1 | SUPERFAMILY | SSF82109 | MIR domain | 402 | 584 | 2.88E-56 |
| 22 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 66 | 88 | - |
| 23 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 141 | - |
| 24 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 210 | 227 | - |
| 25 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 234 | 256 | - |
| 21 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 313 | 335 | - |
| 20 | g2097.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 348 | 370 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006493 | protein O-linked glycosylation | BP |
| GO:0000030 | mannosyltransferase activity | MF |
| GO:0005789 | endoplasmic reticulum membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K00728 (dolichyl-phosphate-mannose-protein mannosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
