Gene loci information

Transcript annotation

  • This transcript has been annotated as Dual specificity mitogen-activated protein kinase kinase dSOR1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2128 g2128.t7 TSS g2128.t7 15354050 15354050
chr_3 g2128 g2128.t7 isoform g2128.t7 15354188 15356042
chr_3 g2128 g2128.t7 exon g2128.t7.exon1 15354188 15354267
chr_3 g2128 g2128.t7 cds g2128.t7.CDS1 15354188 15354267
chr_3 g2128 g2128.t7 exon g2128.t7.exon2 15354335 15354530
chr_3 g2128 g2128.t7 cds g2128.t7.CDS2 15354335 15354530
chr_3 g2128 g2128.t7 exon g2128.t7.exon3 15354588 15354767
chr_3 g2128 g2128.t7 cds g2128.t7.CDS3 15354588 15354767
chr_3 g2128 g2128.t7 exon g2128.t7.exon4 15354826 15355416
chr_3 g2128 g2128.t7 cds g2128.t7.CDS4 15354826 15355416
chr_3 g2128 g2128.t7 exon g2128.t7.exon5 15355473 15356042
chr_3 g2128 g2128.t7 cds g2128.t7.CDS5 15355473 15355583
chr_3 g2128 g2128.t7 TTS g2128.t7 NA NA

Sequences

>g2128.t7 Gene=g2128 Length=1617
ATGTCTAAGAACAAGCTAAATTTGACATTGCCTCCGCCTTCTATCGATGCTTCGATCACT
CCAGCTTTAGCTTCTGCAGAGAAAAAAGAATTACTTGGTACAAAAAATAGTATTGATCAA
TTAACAGAGACTTTAGAAGAACTCGATATGGATGAGAATGCACGCAAAAGGATAAAAGTA
TTTTTAAATCAAAAAGAGAAAATTGGTGAACTCTCAGATCAAGATCTTGAAAAATTAGGA
GAGTTAGGTTCTGGAAATGGAGGTGTAGTGATGATGGCACGACACAAACCAACTTCTCTC
ATAATGGCAAGAAAAATGATTCATCTTGAAGTAAAGCCAGCTATTAAGAAACAAATTATT
CGTGAATTGAAAGTATTGCATGAATGTAATTTTCCTCATATTGTTGGTTTCTATGGAGCC
TTTTATAGTGATGGCGAAATCAGCATTTGCATGGAGTATATGGACGGTGGTTCACTTGAT
TTGATTTTAAAACGTGCTGGACGTATACATGAGAACATTTTGGGCAAAATAACAGTTGCT
GTGTTAAAAGGTTTAACTTATTTACGTGATAAACATGCCATCATTCATAGAGATGTCAAA
CCGAGTAATATTTTGGTTAATAGCAGTGGAGAAATAAAAATTTGTGATTTTGGTGTAAGT
GGTCAATTGATTGATTCAATGGCAAACTCATTTGTCGGCACACGTAGCTATATGTCACCT
GAACGTTTACAAGGAACACATTATTCAGTTCAATCAGACATTTGGTCCTTAGGCCTCTCA
TTAGTGGAGATGGCTATAGGAATGTATCCAATTCCACCACCTGACATGCAGACACTTGAT
GCTATTCTCGCAAACGATGCACAATCATTAGCAAATCAGACAATTATTGAGCCACGATCG
ATGGCAATTTTTGAATTATTAGACTATATTGTGAATGAGCCACCACCGAAACTAGAGCAT
CCTAGTTTCTCGCCCAATTTCAAAGACTTTGTAGATAAATGTTTGAAGAAAAATCCTGAA
GAACGTGCTGATTTAAAGACATTAATGAAGGCTCATGATTGGATAAGACATTCTGAAGAA
GAAAATGTTGATGTAGCTGGTTGGGTTTGTCGAACTATGAATTTGCCTCTTACTCCAAAA
CGCACGATACCACAGTAATTCCCTTGATAAATAAATTAATTAATCATTACTCATCCAGTC
ATAATATAACTTTTAATACTTATTAATATTTAAAAAAAGGAAAAATCATAAAAGAAAAGA
AAAACCAACCTACCTAATTATAAAGATTTTAATGACAATTTCTTTGTCTATTATTCGAAG
AAAAAAATTGGCAAACTATTTTAAAAAAATGTTTAAAATTTTCTTCATATATTCATTATA
CTCTTTGTGTAATATGACAATGTTATACATATTGGAAAAGAAACTAAAGTACATAAGAAG
GCAGATGAACGAGCAAAATATGTTTCAAATTTAAAATGAATGTAAAGATTTTTTCGCATA
ATTTAATGAAGCAAAAATCGCAAGAAAAAAATTCATTGCAAATGAGTTCTTATACTTGAA
ACAAAATTGTTTTATTTTATTTTAAAAAAGATATAATTAATGTTTAACAAAATTCTT

>g2128.t7 Gene=g2128 Length=385
MSKNKLNLTLPPPSIDASITPALASAEKKELLGTKNSIDQLTETLEELDMDENARKRIKV
FLNQKEKIGELSDQDLEKLGELGSGNGGVVMMARHKPTSLIMARKMIHLEVKPAIKKQII
RELKVLHECNFPHIVGFYGAFYSDGEISICMEYMDGGSLDLILKRAGRIHENILGKITVA
VLKGLTYLRDKHAIIHRDVKPSNILVNSSGEIKICDFGVSGQLIDSMANSFVGTRSYMSP
ERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIPPPDMQTLDAILANDAQSLANQTIIEPRS
MAIFELLDYIVNEPPPKLEHPSFSPNFKDFVDKCLKKNPEERADLKTLMKAHDWIRHSEE
ENVDVAGWVCRTMNLPLTPKRTIPQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g2128.t7 CDD cd06615 PKc_MEK 75 373 0.0
7 g2128.t7 Coils Coil Coil 31 51 -
5 g2128.t7 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 42 155 9.8E-46
6 g2128.t7 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 156 380 5.6E-65
2 g2128.t7 PANTHER PTHR48013:SF19 DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1 1 376 1.2E-187
3 g2128.t7 PANTHER PTHR48013 DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED 1 376 1.2E-187
1 g2128.t7 Pfam PF00069 Protein kinase domain 77 354 1.6E-65
10 g2128.t7 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 82 105 -
9 g2128.t7 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 194 206 -
12 g2128.t7 ProSiteProfiles PS50011 Protein kinase domain profile. 76 355 45.827
11 g2128.t7 SMART SM00220 serkin_6 76 355 4.7E-82
4 g2128.t7 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 70 352 2.83E-77

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values