Gene loci information

Transcript annotation

  • This transcript has been annotated as Eukaryotic translation initiation factor 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2176 g2176.t10 isoform g2176.t10 15791791 15793413
chr_3 g2176 g2176.t10 exon g2176.t10.exon1 15791791 15792313
chr_3 g2176 g2176.t10 cds g2176.t10.CDS1 15791792 15792124
chr_3 g2176 g2176.t10 exon g2176.t10.exon2 15792418 15792514
chr_3 g2176 g2176.t10 exon g2176.t10.exon3 15792947 15793249
chr_3 g2176 g2176.t10 exon g2176.t10.exon4 15793314 15793413
chr_3 g2176 g2176.t10 TSS g2176.t10 NA NA
chr_3 g2176 g2176.t10 TTS g2176.t10 NA NA

Sequences

>g2176.t10 Gene=g2176 Length=1023
GAACCCTGTAATTTTAATTCAGACAAAAACAAAATTTTATAAAAATATTTCTTATCTTAA
TTAATTAACAAATTTTGCTTAAATAATCTTTATATTTCATAAAACTTAATAAACAAAATT
TCGCCATTAATTCAAAGATTCGCCGTAGTTTGAAAAGCGACAATATTGAATCAAAAGATA
AGATATTGAGAAAATTTTATTCGTAAAATGTTTTCATCTTATTCTTGTCGTGTGAAAGTT
TTATATCGTCAAAATAATACCAAACGATAGATAAATAACACCAATATTCTATGTAATTGG
AGTTTAAAAGTCACTTTTTGAGGCAATCGAGAAAAATATAAATCATCAAGTTTTTAAGCT
TATCGGGGGATTTTATGAATTTAAAAAACACATTTGAAGGTCAAAAACTTTGATCAGATC
GAACAGCCGATATTTTTAAATTTATCACATCAGAATCCTTTAAAGTGCATGTCAGCGAAT
TTAAATCAGTTGAGTCATAGGACTTCAAAATTTTCAAGCAATCGACATCGGAAATTGCAA
CCATTTAGAAAAACATCGTGAATTAGTCATAAACATTGAGTAAAGTGAACAAGAGTTCTA
TTATTATCATCATCATCACCATCATCAAAGAATATAATACATAATCAAAAAAATAAGGGA
ACGTAATTCAAATTTAATTTGATAGAAGGATGGGGACTTTGAATGTCAATCGTAATGTTA
CGGACTTGTATTATCGCTACAAGATGCCACGTATTTGTGCCAAAGTAGAAGGCAAGGGCA
ATGGCATTAAGACCGTTATTGTCAATATGGCTGAAGTAGCTAAAGCACTCAATCGTCCTG
CTACTTATCCAACTAAATACTTTGGTTGCGAGCTTGGCGCTCAGACTCAATTCGATCATA
AGAATGAACGTTTTATTGTCAATGGCTCGCATGATGCATCAAAATTGCAAGATCTTCTTG
ATGGATTTATTCGCAAATATGTCTTGTGTCCAGAATGCGACAATCCTGAGACCGATTTAA
TCA

>g2176.t10 Gene=g2176 Length=111
MGTLNVNRNVTDLYYRYKMPRICAKVEGKGNGIKTVIVNMAEVAKALNRPATYPTKYFGC
ELGAQTQFDHKNERFIVNGSHDASKLQDLLDGFIRKYVLCPECDNPETDLI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g2176.t10 Gene3D G3DSA:3.30.30.50 Translation initiation factor 2 beta 23 99 0
2 g2176.t10 PANTHER PTHR23001:SF22 EUKARYOTIC TRANSLATION INITIATION FACTOR 5 4 110 0
3 g2176.t10 PANTHER PTHR23001 EUKARYOTIC TRANSLATION INITIATION FACTOR 4 110 0
1 g2176.t10 Pfam PF01873 Domain found in IF2B/IF5 9 111 0
5 g2176.t10 SMART SM00653 eIF2Bneu4 14 111 0
4 g2176.t10 SUPERFAMILY SSF100966 Translation initiation factor 2 beta, aIF2beta, N-terminal domain 5 100 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006413 translational initiation BP
GO:0003743 translation initiation factor activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values