General information for g218.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit STT3B.
Parent gene
- The parent gene g218 is located on chr_3: 1821520..1823407 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g218 | g218.t3 | isoform | g218.t3 | 1819488 | 1823407 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon1 | 1819488 | 1820652 |
| chr_3 | g218 | g218.t3 | TTS | g218.t3 | 1819489 | 1819489 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon2 | 1820719 | 1820911 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon3 | 1820975 | 1821364 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon4 | 1821498 | 1822067 |
| chr_3 | g218 | g218.t3 | cds | g218.t3.CDS1 | 1821503 | 1822067 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon5 | 1822119 | 1822944 |
| chr_3 | g218 | g218.t3 | cds | g218.t3.CDS2 | 1822119 | 1822944 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon6 | 1823005 | 1823093 |
| chr_3 | g218 | g218.t3 | cds | g218.t3.CDS3 | 1823005 | 1823093 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon7 | 1823173 | 1823192 |
| chr_3 | g218 | g218.t3 | cds | g218.t3.CDS4 | 1823173 | 1823192 |
| chr_3 | g218 | g218.t3 | exon | g218.t3.exon8 | 1823258 | 1823407 |
| chr_3 | g218 | g218.t3 | cds | g218.t3.CDS5 | 1823258 | 1823407 |
| chr_3 | g218 | g218.t3 | TSS | g218.t3 | 1823553 | 1823553 |
Sequences
>g218.t3 Gene=g218 Length=3403
ATGACTGAAACGCCAGTTAAAGTTAAATCAGGGTCTGCAAAAGGAAATTTTGTAGGAATC
TCTAGTTTGATTGTTTTCTCGGTTCTACTTGTTTGCTGGATTGCCGGCTTTGCATCGAGA
CTGTTTGCTGTCATTAGATTCGAGTCAATCATCCATGAGTTCGACCCTTGGTTTAACTAT
CGTGCCACAGCTTACATGGTCGAACATGGTTTTTATAAATTTCTTAATTGGTTTGATGAA
CGTGCATGGTATCCACTAGGTCGAATTGTTGGTGGAACTGTGTATCCTGGTCTTATGATT
ACTTCTGGTGGAATTCATTGGTTATTACATCTTCTCAATATTCCCGTATATATTCGTGAT
ATTTGTGTGTTCCTTGCACCAATTTTTAGTGGTCTCACTGCAATTTCAACTTATCTTTTG
ACCAAAGAATTATGGAGTCCAGGTGCTGGATTATTTGCAGCTTCATTTATAGCTATCGTT
CCTGGTTATATTTCAAGATCAGTTGCTGGATCATATGACAACGAAGGAATTGCCATTTTT
GCACTTCAATTTACGTATTACCTTTGGGTGAAATCTGTAAAAACAGGCTCTATCTTTTGG
GCTGCTTCAACTGCTCTCTCCTATTTCTATATGGTTTCTGCTTGGGGAGGATATGTTTTC
ATTATCAATCTTATTCCATTACACGTGTTTGTGTTGCTCATCATGAATCGTTATTCGCAT
CGTTTGTTTACTAGCTACACAACATTCTACATTCTTGGACTCATCTTTTCAATGCAAATT
CCATTCGTCGGCTTCCAACCGATTCGAACGAGTGAGCACATGGCTGCATCCGGTGTTTTT
GCTCTTCTCATAGCTGTCGGGATTTTGAAACATTTACAAACCGTCCTTACAAAGCAAGAA
TTCAAGCGATTATTTATTCTCGGCGGTCTCACTGCCGCTGGCATAGTATTTATGACTGTT
GTTGGTCTCACAATGGCTGGAGTTATTGCACCATGGTCAGGACGTTTCTACTCACTTTGG
GACACTGGGTATGCTAAAATTCACATTCCAATCATTGCTTCAGTTTCTGAACATCAACCT
ACAACGTGGTTTTCTTTCTTCTTCGATCTGCACATTCTTGTGTGCACTTTCCCCGTCGGA
TTATGGTATGCTATAAAGAAAATCAACGATGAGCGTGTTTTTGTTGTGCTCTATGCGATC
TCAGCAGTATATTTCGCTGGTGTGATGGTTCGATTGATGTTGACTTTGACACCAGTTGTT
TGCATTCTGGCGGGTGTTGCATTCTCAAGTCTTTTGGAAGTCTTCCTTAAAGAAGATCAA
CCAATTACTCAAAAGGATGAAACAGAACAAGAAGAACCAGCCGAAAAGAAGCTATATGAT
AAGGCAGGAAAAATTAAGCCAAGAAAGCATGATAACAACAAAAATGAGAATAAAGGTCTT
GGGTCAAATATTAAATCAATGGTAGTTATTGCAATTTTGATGCTACTTATGATGTTCGCT
GTACATTGTACATGGGTCACTAGCAATGCATACTCTTCACCATCAATTGTATTGGCATTT
TACAATCAAGGCGATGGCTCAAGAAATATTCTCGATGATTTCCGTGAAGCATATTATTGG
CTCTGGCAAAATACTGAAGATGTAAGATAAATTTATTGGTGGGATTATGGCTATCAGATA
GCGGGAATGGCGAATCGGACAACGCTAGTAGACAATAATACGTGGAATAATAGTCATATA
GCTTTAGTTGGAAAAGCAATGTCATCGTCAGAAGAAAAAGCTCATGAAATTTACACATCT
TTAGGTGCTGATTATGTTCTTGTAATCTTTGGTGGTGTGATTGGTTATTCAGGCGATGAC
ATTAATAAGTTTTTGTGGATGGTACGAATTGCCGAAGGCGAGCATCCAAAAGATATAAGA
GAAAGTGACTACTTTACTGAACGTGGTGAATTCAGAGTTGATTCAGAAGGTTCACCTACA
CTTCTTAATTGCTTGATGTACAAGCTAAGTTATTATAGATTTGGTGAATTAAAACTTGAT
TATAGAGGACCAGCTGGTTTTGATCGTACAAGAAATGCAGTAATTGGTAATAAAGATTTT
GATTTAACTTACTTTGAAGAGGTCTACACTAGCGAGCACTGGCTGGTGCGTATTTATCGT
GTTAAGAAACCAGACGAATTCAATCGTCCATCGCTCAAACCACAGAAGCGCTCTGGTTTT
GATTCAATCACAAAAAAAACATCAAAACGAAGAAAAGGCGTCATCAAAAATCGTCCTGTA
GTGGTGAAAGGCAAACGGTCAACGATAAAAGCCAACTAGATTAAACAATGAATCACAGAA
GCACATACACAAACAAACACACTATAGACAGATTTATTTCACTTTTTCTGCCAAAAATCC
AATCAATATTAACTACTAAAACAGATAGAAGAATTGTTTCTTTATATTTCTCCATGATTT
TTGGGTATTTCTATTCATTGTTGTTTCTTTTTTTCACATACACACACATAGATTTCAAAT
AAATGAATGGATGAATAGAGAATAAAAAAACTGGGGTGGAATGAATTTTGACTTTATAAA
ATATATTGAGGAGATGAAATACAATTTTCTTTCCTCTTATAATAAGAACAAAAAAATAAA
TAAAAGAAAAGTGCGAGAGTGAAACGGAAAACATTTTTATTCGTCGTGGCCTTAAATTCA
TCATCAAAAAGAAAGAAAAAGGATGAGGGGTAGTGGAAATTGCAACGATGATGGCGAGTA
GGAAGAGAAATGGTTGTTATGAGGCGAGAAAAAGAAAAACAAAAATGCAAACAAACAAAC
ACTTCAAGTGTTGTTGATCGATAATACACAGAAAAAAAGTATAATGCGAAAAGTGAAGGC
ATAAAAAGAAAAACAAACATTGGGAGAGAGAGAACATTTCCTGAAAAAAAAACAGCAATT
GTATTGAAAAAATTCCATTTTAAAAATGTTGAAGGACTTTCGTCCAGTAGTGTTTTAAGT
TCGATAAAAAAAAGTTTCATGCATTTATTTTATTAATTTGAATTTTTTATTTTTGTTTGC
TGTTTTCCATTAAAAATTATTTCACGAGTCATGACCACCCCTAGCACATAAACACTTTTA
TTATTTTTGATTTTGAGACTGCACAATATTGTGAATTGAAGTATCTTTTTGGTTTTTTTT
AAAAAATCACAAAAAACATGAATATACATCGATAAAATATCATATATACCCCCCATACTA
TTGAGAACTGAAAAAGAAAATAATATTTAAAATAATAATAAAATTGTGTTGTAGATTTTT
AAAGTAATTCGTTTAATTTGATGAAAATATTCAATGTCGTCTTTTTACCTTTAAAAATTT
TTAGTATATGAAATCTATTTGAAATAAATCTAGTTAGAAATTA
>g218.t3 Gene=g218 Length=549
MTETPVKVKSGSAKGNFVGISSLIVFSVLLVCWIAGFASRLFAVIRFESIIHEFDPWFNY
RATAYMVEHGFYKFLNWFDERAWYPLGRIVGGTVYPGLMITSGGIHWLLHLLNIPVYIRD
ICVFLAPIFSGLTAISTYLLTKELWSPGAGLFAASFIAIVPGYISRSVAGSYDNEGIAIF
ALQFTYYLWVKSVKTGSIFWAASTALSYFYMVSAWGGYVFIINLIPLHVFVLLIMNRYSH
RLFTSYTTFYILGLIFSMQIPFVGFQPIRTSEHMAASGVFALLIAVGILKHLQTVLTKQE
FKRLFILGGLTAAGIVFMTVVGLTMAGVIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQP
TTWFSFFFDLHILVCTFPVGLWYAIKKINDERVFVVLYAISAVYFAGVMVRLMLTLTPVV
CILAGVAFSSLLEVFLKEDQPITQKDETEQEEPAEKKLYDKAGKIKPRKHDNNKNENKGL
GSNIKSMVVIAILMLLMMFAVHCTWVTSNAYSSPSIVLAFYNQGDGSRNILDDFREAYYW
LWQNTEDVR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g218.t3 | PANTHER | PTHR13872 | DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT | 21 | 547 | 4.3E-217 |
| 1 | g218.t3 | Pfam | PF02516 | Oligosaccharyl transferase STT3 subunit | 28 | 512 | 6.4E-140 |
| 19 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 16 | - |
| 35 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 17 | 38 | - |
| 26 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 39 | 88 | - |
| 31 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 89 | 109 | - |
| 20 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 110 | 120 | - |
| 37 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 121 | 139 | - |
| 28 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 140 | 144 | - |
| 39 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 145 | 164 | - |
| 21 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 165 | 175 | - |
| 38 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 176 | 202 | - |
| 27 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 203 | 207 | - |
| 32 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 208 | 235 | - |
| 18 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 236 | 246 | - |
| 41 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 247 | 268 | - |
| 25 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 269 | 273 | - |
| 42 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 274 | 292 | - |
| 22 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 293 | 303 | - |
| 34 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 304 | 330 | - |
| 24 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 331 | 362 | - |
| 36 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 363 | 385 | - |
| 17 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 386 | 391 | - |
| 30 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 392 | 410 | - |
| 23 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 411 | 415 | - |
| 40 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 416 | 436 | - |
| 16 | g218.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 437 | 486 | - |
| 33 | g218.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 487 | 507 | - |
| 29 | g218.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 508 | 549 | - |
| 11 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 17 | 39 | - |
| 12 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 92 | 114 | - |
| 14 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 121 | 140 | - |
| 5 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 145 | 167 | - |
| 6 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 180 | 202 | - |
| 10 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 212 | 234 | - |
| 15 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 241 | 263 | - |
| 9 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 273 | 292 | - |
| 13 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 304 | 326 | - |
| 8 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 363 | 385 | - |
| 4 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 392 | 410 | - |
| 7 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 414 | 436 | - |
| 3 | g218.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 479 | 501 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006486 | protein glycosylation | BP |
| GO:0004576 | oligosaccharyl transferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
