Gene loci information

Transcript annotation

  • This transcript has been annotated as Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit STT3B.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g218 g218.t5 isoform g218.t5 1820095 1823407
chr_3 g218 g218.t5 exon g218.t5.exon1 1820095 1820652
chr_3 g218 g218.t5 TTS g218.t5 1820103 1820103
chr_3 g218 g218.t5 exon g218.t5.exon2 1820719 1820911
chr_3 g218 g218.t5 exon g218.t5.exon3 1820975 1821364
chr_3 g218 g218.t5 exon g218.t5.exon4 1821480 1822067
chr_3 g218 g218.t5 cds g218.t5.CDS1 1821503 1822067
chr_3 g218 g218.t5 exon g218.t5.exon5 1822119 1822944
chr_3 g218 g218.t5 cds g218.t5.CDS2 1822119 1822944
chr_3 g218 g218.t5 exon g218.t5.exon6 1823005 1823093
chr_3 g218 g218.t5 cds g218.t5.CDS3 1823005 1823093
chr_3 g218 g218.t5 exon g218.t5.exon7 1823173 1823192
chr_3 g218 g218.t5 cds g218.t5.CDS4 1823173 1823192
chr_3 g218 g218.t5 exon g218.t5.exon8 1823258 1823407
chr_3 g218 g218.t5 cds g218.t5.CDS5 1823258 1823407
chr_3 g218 g218.t5 TSS g218.t5 1823553 1823553

Sequences

>g218.t5 Gene=g218 Length=2814
ATGACTGAAACGCCAGTTAAAGTTAAATCAGGGTCTGCAAAAGGAAATTTTGTAGGAATC
TCTAGTTTGATTGTTTTCTCGGTTCTACTTGTTTGCTGGATTGCCGGCTTTGCATCGAGA
CTGTTTGCTGTCATTAGATTCGAGTCAATCATCCATGAGTTCGACCCTTGGTTTAACTAT
CGTGCCACAGCTTACATGGTCGAACATGGTTTTTATAAATTTCTTAATTGGTTTGATGAA
CGTGCATGGTATCCACTAGGTCGAATTGTTGGTGGAACTGTGTATCCTGGTCTTATGATT
ACTTCTGGTGGAATTCATTGGTTATTACATCTTCTCAATATTCCCGTATATATTCGTGAT
ATTTGTGTGTTCCTTGCACCAATTTTTAGTGGTCTCACTGCAATTTCAACTTATCTTTTG
ACCAAAGAATTATGGAGTCCAGGTGCTGGATTATTTGCAGCTTCATTTATAGCTATCGTT
CCTGGTTATATTTCAAGATCAGTTGCTGGATCATATGACAACGAAGGAATTGCCATTTTT
GCACTTCAATTTACGTATTACCTTTGGGTGAAATCTGTAAAAACAGGCTCTATCTTTTGG
GCTGCTTCAACTGCTCTCTCCTATTTCTATATGGTTTCTGCTTGGGGAGGATATGTTTTC
ATTATCAATCTTATTCCATTACACGTGTTTGTGTTGCTCATCATGAATCGTTATTCGCAT
CGTTTGTTTACTAGCTACACAACATTCTACATTCTTGGACTCATCTTTTCAATGCAAATT
CCATTCGTCGGCTTCCAACCGATTCGAACGAGTGAGCACATGGCTGCATCCGGTGTTTTT
GCTCTTCTCATAGCTGTCGGGATTTTGAAACATTTACAAACCGTCCTTACAAAGCAAGAA
TTCAAGCGATTATTTATTCTCGGCGGTCTCACTGCCGCTGGCATAGTATTTATGACTGTT
GTTGGTCTCACAATGGCTGGAGTTATTGCACCATGGTCAGGACGTTTCTACTCACTTTGG
GACACTGGGTATGCTAAAATTCACATTCCAATCATTGCTTCAGTTTCTGAACATCAACCT
ACAACGTGGTTTTCTTTCTTCTTCGATCTGCACATTCTTGTGTGCACTTTCCCCGTCGGA
TTATGGTATGCTATAAAGAAAATCAACGATGAGCGTGTTTTTGTTGTGCTCTATGCGATC
TCAGCAGTATATTTCGCTGGTGTGATGGTTCGATTGATGTTGACTTTGACACCAGTTGTT
TGCATTCTGGCGGGTGTTGCATTCTCAAGTCTTTTGGAAGTCTTCCTTAAAGAAGATCAA
CCAATTACTCAAAAGGATGAAACAGAACAAGAAGAACCAGCCGAAAAGAAGCTATATGAT
AAGGCAGGAAAAATTAAGCCAAGAAAGCATGATAACAACAAAAATGAGAATAAAGGTCTT
GGGTCAAATATTAAATCAATGGTAGTTATTGCAATTTTGATGCTACTTATGATGTTCGCT
GTACATTGTACATGGGTCACTAGCAATGCATACTCTTCACCATCAATTGTATTGGCATTT
TACAATCAAGGCGATGGCTCAAGAAATATTCTCGATGATTTCCGTGAAGCATATTATTGG
CTCTGGCAAAATACTGAAGATGTAAGATAAATTTAGTTTTTAATTTTTCACATTTGGTGG
GATTATGGCTATCAGATAGCGGGAATGGCGAATCGGACAACGCTAGTAGACAATAATACG
TGGAATAATAGTCATATAGCTTTAGTTGGAAAAGCAATGTCATCGTCAGAAGAAAAAGCT
CATGAAATTTACACATCTTTAGGTGCTGATTATGTTCTTGTAATCTTTGGTGGTGTGATT
GGTTATTCAGGCGATGACATTAATAAGTTTTTGTGGATGGTACGAATTGCCGAAGGCGAG
CATCCAAAAGATATAAGAGAAAGTGACTACTTTACTGAACGTGGTGAATTCAGAGTTGAT
TCAGAAGGTTCACCTACACTTCTTAATTGCTTGATGTACAAGCTAAGTTATTATAGATTT
GGTGAATTAAAACTTGATTATAGAGGACCAGCTGGTTTTGATCGTACAAGAAATGCAGTA
ATTGGTAATAAAGATTTTGATTTAACTTACTTTGAAGAGGTCTACACTAGCGAGCACTGG
CTGGTGCGTATTTATCGTGTTAAGAAACCAGACGAATTCAATCGTCCATCGCTCAAACCA
CAGAAGCGCTCTGGTTTTGATTCAATCACAAAAAAAACATCAAAACGAAGAAAAGGCGTC
ATCAAAAATCGTCCTGTAGTGGTGAAAGGCAAACGGTCAACGATAAAAGCCAACTAGATT
AAACAATGAATCACAGAAGCACATACACAAACAAACACACTATAGACAGATTTATTTCAC
TTTTTCTGCCAAAAATCCAATCAATATTAACTACTAAAACAGATAGAAGAATTGTTTCTT
TATATTTCTCCATGATTTTTGGGTATTTCTATTCATTGTTGTTTCTTTTTTTCACATACA
CACACATAGATTTCAAATAAATGAATGGATGAATAGAGAATAAAAAAACTGGGGTGGAAT
GAATTTTGACTTTATAAAATATATTGAGGAGATGAAATACAATTTTCTTTCCTCTTATAA
TAAGAACAAAAAAATAAATAAAAGAAAAGTGCGAGAGTGAAACGGAAAACATTTTTATTC
GTCGTGGCCTTAAATTCATCATCAAAAAGAAAGAAAAAGGATGAGGGGTAGTGGAAATTG
CAACGATGATGGCGAGTAGGAAGAGAAATGGTTGTTATGAGGCGAGAAAAAGAA

>g218.t5 Gene=g218 Length=549
MTETPVKVKSGSAKGNFVGISSLIVFSVLLVCWIAGFASRLFAVIRFESIIHEFDPWFNY
RATAYMVEHGFYKFLNWFDERAWYPLGRIVGGTVYPGLMITSGGIHWLLHLLNIPVYIRD
ICVFLAPIFSGLTAISTYLLTKELWSPGAGLFAASFIAIVPGYISRSVAGSYDNEGIAIF
ALQFTYYLWVKSVKTGSIFWAASTALSYFYMVSAWGGYVFIINLIPLHVFVLLIMNRYSH
RLFTSYTTFYILGLIFSMQIPFVGFQPIRTSEHMAASGVFALLIAVGILKHLQTVLTKQE
FKRLFILGGLTAAGIVFMTVVGLTMAGVIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQP
TTWFSFFFDLHILVCTFPVGLWYAIKKINDERVFVVLYAISAVYFAGVMVRLMLTLTPVV
CILAGVAFSSLLEVFLKEDQPITQKDETEQEEPAEKKLYDKAGKIKPRKHDNNKNENKGL
GSNIKSMVVIAILMLLMMFAVHCTWVTSNAYSSPSIVLAFYNQGDGSRNILDDFREAYYW
LWQNTEDVR

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g218.t5 PANTHER PTHR13872 DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 21 547 4.3E-217
1 g218.t5 Pfam PF02516 Oligosaccharyl transferase STT3 subunit 28 512 6.4E-140
19 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 16 -
35 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 17 38 -
26 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 39 88 -
31 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 89 109 -
20 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 110 120 -
37 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 121 139 -
28 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 140 144 -
39 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 145 164 -
21 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 165 175 -
38 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 176 202 -
27 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 203 207 -
32 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 208 235 -
18 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 236 246 -
41 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 247 268 -
25 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 269 273 -
42 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 274 292 -
22 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 293 303 -
34 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 304 330 -
24 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 331 362 -
36 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 363 385 -
17 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 386 391 -
30 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 392 410 -
23 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 411 415 -
40 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 416 436 -
16 g218.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 437 486 -
33 g218.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 487 507 -
29 g218.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 508 549 -
11 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 17 39 -
12 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 92 114 -
14 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 121 140 -
5 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 145 167 -
6 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 180 202 -
10 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 212 234 -
15 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 241 263 -
9 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 273 292 -
13 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 304 326 -
8 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 363 385 -
4 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 392 410 -
7 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 414 436 -
3 g218.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 479 501 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0016020 membrane CC
GO:0006486 protein glycosylation BP
GO:0004576 oligosaccharyl transferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values