Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a14.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2249 g2249.t1 TSS g2249.t1 16276375 16276375
chr_3 g2249 g2249.t1 isoform g2249.t1 16276405 16278663
chr_3 g2249 g2249.t1 exon g2249.t1.exon1 16276405 16276754
chr_3 g2249 g2249.t1 cds g2249.t1.CDS1 16276405 16276754
chr_3 g2249 g2249.t1 exon g2249.t1.exon2 16277421 16277653
chr_3 g2249 g2249.t1 cds g2249.t1.CDS2 16277421 16277653
chr_3 g2249 g2249.t1 exon g2249.t1.exon3 16277711 16278010
chr_3 g2249 g2249.t1 cds g2249.t1.CDS3 16277711 16278010
chr_3 g2249 g2249.t1 exon g2249.t1.exon4 16278074 16278663
chr_3 g2249 g2249.t1 cds g2249.t1.CDS4 16278074 16278663
chr_3 g2249 g2249.t1 TTS g2249.t1 16278750 16278750

Sequences

>g2249.t1 Gene=g2249 Length=1473
ATGTTCGTCTTTGTTTCTTTAATATTTTTATTCATCATTTCAATTTATTTTTGGATAAAG
AAAATTTACAGCTATTGGAGTGAAAAGGGATTTTTACAAAATTCAAATGTGAAATTTCCA
TTTGGCTCTTTAACTGGTGTTGGTAAAAAATTTACAACTTGTGAAGCATTAGACATAATT
TACAGAGAAATTAAAGGAAAATCTCCTGTAGTTGGAATTTACTTCTTCTTTAAACCAACT
TTGATGGTCATCGACCCAGAGTTAATTAAGAAAATATTTGTTCAAGATTTTACATACTTT
CAAGATCGAGGATTTTATCACAACAAAAATTCTGACCCAATATCAGCAAATTTGCTCACT
TTAGAAGGTCAAGAATGGAAAGAACGCCGTAGTAAATTATCTCCAATTTTTAGCTCTGGA
AAGATGAAAATGATGTTTCATATTGTTGATTTGATCAGTTCGAGATTAATCGAAGCAATT
GAAAAAGATTTACAAAATTCAAGTGACCTTGAAATGAGAAATTGGGCACAAAGGTTTACA
GCTGATAATATTAGTAGCACTGCATTCGGTTTAGATGCGAAATGCTTAGAAGATGAAAAT
TCTGAATTTTTAAAACATGGTAGAAGACTTTTTGATTTGACACCTTTTGAAACATTAAAA
TTTTTCTTCACAATTGGCTGTCCAAATTTTTGTAGAAAATTAGGAGTCAGAACAAATCCA
AAAGAACCAACAGATTTTTTCCTAAAAACATTTTTGGAAACATTTGAATATCGCGAGAAA
AACAATATTCAGAGAAATGATTTTGTTTCACTATTGCTTGGATTGAAAGAATTATTGACA
CCAATTGAATTAGCAGCTGAGGCATTTTTGATGTTTGCAGCTGGATATGAAACAAGTTCA
ACATTGATGACTTTCATACTTTATGAGCTTGCACTTAATCCAGACATTCAACAAAAATTA
AGAGATGAAATTGTTACAAGAATTGAGGAAAATGATGGAAAATTAACTTATGAAATGTTG
AATGAATTCAAATATCTTGACATGGTGACTAATGAAACTTTAAGAAAATATCCTCCGATT
CCGAATAATATTCGAATGTGCACAAAAGATTACAAAATTGAAGAGCTTAATTTGACTATT
CCTAAAGGAACAGCCATTGAATTGCCAGTTTATTCACTTCAACACGATCCTGAATATTTT
CCAAATCCAAACACATTTGATCCTGAAAGATTTAACGATGAAAATGTCAAAAATATCAAA
CAATTTACATTCTTTCCATTTGGAGAAGGCAAAAGACAATGTTTAGGTTTAAGATTTGGT
CTGATGCAAACCAAAATGGGCATTGCAAAATTGTTAATAAATTATTCATTTTCATCTTGT
GAAAAGACCATAATTCCAATGAAATTTGTACCAAGTGCTCCTTTTTTGCAACCAAAAGGT
GGATTGTGGTTGAAAGTTGAAAAAATTTTATAA

>g2249.t1 Gene=g2249 Length=490
MFVFVSLIFLFIISIYFWIKKIYSYWSEKGFLQNSNVKFPFGSLTGVGKKFTTCEALDII
YREIKGKSPVVGIYFFFKPTLMVIDPELIKKIFVQDFTYFQDRGFYHNKNSDPISANLLT
LEGQEWKERRSKLSPIFSSGKMKMMFHIVDLISSRLIEAIEKDLQNSSDLEMRNWAQRFT
ADNISSTAFGLDAKCLEDENSEFLKHGRRLFDLTPFETLKFFFTIGCPNFCRKLGVRTNP
KEPTDFFLKTFLETFEYREKNNIQRNDFVSLLLGLKELLTPIELAAEAFLMFAAGYETSS
TLMTFILYELALNPDIQQKLRDEIVTRIEENDGKLTYEMLNEFKYLDMVTNETLRKYPPI
PNNIRMCTKDYKIEELNLTIPKGTAIELPVYSLQHDPEYFPNPNTFDPERFNDENVKNIK
QFTFFPFGEGKRQCLGLRFGLMQTKMGIAKLLINYSFSSCEKTIIPMKFVPSAPFLQPKG
GLWLKVEKIL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g2249.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 489 3.8E-130
2 g2249.t1 PANTHER PTHR24292 CYTOCHROME P450 4 489 2.0E-167
3 g2249.t1 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 4 489 2.0E-167
8 g2249.t1 PRINTS PR00463 E-class P450 group I signature 283 300 2.9E-22
10 g2249.t1 PRINTS PR00385 P450 superfamily signature 294 311 4.2E-10
7 g2249.t1 PRINTS PR00463 E-class P450 group I signature 303 329 2.9E-22
4 g2249.t1 PRINTS PR00463 E-class P450 group I signature 347 365 2.9E-22
12 g2249.t1 PRINTS PR00385 P450 superfamily signature 348 359 4.2E-10
6 g2249.t1 PRINTS PR00463 E-class P450 group I signature 389 413 2.9E-22
5 g2249.t1 PRINTS PR00463 E-class P450 group I signature 424 434 2.9E-22
11 g2249.t1 PRINTS PR00385 P450 superfamily signature 425 434 4.2E-10
9 g2249.t1 PRINTS PR00463 E-class P450 group I signature 434 457 2.9E-22
13 g2249.t1 PRINTS PR00385 P450 superfamily signature 434 445 4.2E-10
1 g2249.t1 Pfam PF00067 Cytochrome P450 60 481 5.0E-96
17 g2249.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
18 g2249.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
19 g2249.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
20 g2249.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 17 -
16 g2249.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 490 -
22 g2249.t1 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 427 436 -
14 g2249.t1 SUPERFAMILY SSF48264 Cytochrome P450 63 487 2.49E-115
21 g2249.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values