Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 6a14.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2249 g2249.t2 TSS g2249.t2 16276375 16276375
chr_3 g2249 g2249.t2 isoform g2249.t2 16276405 16277629
chr_3 g2249 g2249.t2 exon g2249.t2.exon1 16276405 16276754
chr_3 g2249 g2249.t2 cds g2249.t2.CDS1 16276405 16276754
chr_3 g2249 g2249.t2 exon g2249.t2.exon2 16277421 16277629
chr_3 g2249 g2249.t2 cds g2249.t2.CDS2 16277421 16277628
chr_3 g2249 g2249.t2 TTS g2249.t2 NA NA

Sequences

>g2249.t2 Gene=g2249 Length=559
ATGTTCGTCTTTGTTTCTTTAATATTTTTATTCATCATTTCAATTTATTTTTGGATAAAG
AAAATTTACAGCTATTGGAGTGAAAAGGGATTTTTACAAAATTCAAATGTGAAATTTCCA
TTTGGCTCTTTAACTGGTGTTGGTAAAAAATTTACAACTTGTGAAGCATTAGACATAATT
TACAGAGAAATTAAAGGAAAATCTCCTGTAGTTGGAATTTACTTCTTCTTTAAACCAACT
TTGATGGTCATCGACCCAGAGTTAATTAAGAAAATATTTGTTCAAGATTTTACATACTTT
CAAGATCGAGGATTTTATCACAACAAAAATTCTGACCCAATATCAGCAAATTTGCTCACT
TTAGAAGGTCAAGAATGGAAAGAACGCCGTAGTAAATTATCTCCAATTTTTAGCTCTGGA
AAGATGAAAATGATGTTTCATATTGTTGATTTGATCAGTTCGAGATTAATCGAAGCAATT
GAAAAAGATTTACAAAATTCAAGTGACCTTGAAATGAGAAATTGGGCACAAAGGTTTACA
GCTGATAATATTAGTAGCA

>g2249.t2 Gene=g2249 Length=186
MFVFVSLIFLFIISIYFWIKKIYSYWSEKGFLQNSNVKFPFGSLTGVGKKFTTCEALDII
YREIKGKSPVVGIYFFFKPTLMVIDPELIKKIFVQDFTYFQDRGFYHNKNSDPISANLLT
LEGQEWKERRSKLSPIFSSGKMKMMFHIVDLISSRLIEAIEKDLQNSSDLEMRNWAQRFT
ADNISS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g2249.t2 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 186 3.1E-32
2 g2249.t2 PANTHER PTHR24292 CYTOCHROME P450 4 186 8.7E-49
3 g2249.t2 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 4 186 8.7E-49
1 g2249.t2 Pfam PF00067 Cytochrome P450 60 185 2.1E-15
8 g2249.t2 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
9 g2249.t2 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
10 g2249.t2 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
11 g2249.t2 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 17 -
7 g2249.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 186 -
5 g2249.t2 SUPERFAMILY SSF48264 Cytochrome P450 61 185 2.75E-24
4 g2249.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values