Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a14.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2249 g2249.t3 TSS g2249.t3 16276375 16276375
chr_3 g2249 g2249.t3 isoform g2249.t3 16276405 16278663
chr_3 g2249 g2249.t3 exon g2249.t3.exon1 16276405 16276754
chr_3 g2249 g2249.t3 cds g2249.t3.CDS1 16276405 16276754
chr_3 g2249 g2249.t3 exon g2249.t3.exon2 16277484 16277638
chr_3 g2249 g2249.t3 cds g2249.t3.CDS2 16277484 16277638
chr_3 g2249 g2249.t3 exon g2249.t3.exon3 16277711 16278010
chr_3 g2249 g2249.t3 cds g2249.t3.CDS3 16277711 16278010
chr_3 g2249 g2249.t3 exon g2249.t3.exon4 16278074 16278663
chr_3 g2249 g2249.t3 cds g2249.t3.CDS4 16278074 16278663
chr_3 g2249 g2249.t3 TTS g2249.t3 16278750 16278750

Sequences

>g2249.t3 Gene=g2249 Length=1395
ATGTTCGTCTTTGTTTCTTTAATATTTTTATTCATCATTTCAATTTATTTTTGGATAAAG
AAAATTTACAGCTATTGGAGTGAAAAGGGATTTTTACAAAATTCAAATGTGAAATTTCCA
TTTGGCTCTTTAACTGGTGTTGGTAAAAAATTTACAACTTGTGAAGCATTAGACATAATT
TACAGAGAAATTAAAGGAAAATCTCCTGTAGTTGGAATTTACTTCTTCTTTAAACCAACT
TTGATGGTCATCGACCCAGAGTTAATTAAGAAAATATTTGTTCAAGATTTTACATACTTT
CAAGATCGAGGATTTTATCACAACAAAAATTCTGACCCAATATCAGCAAACTCTGGAAAG
ATGAAAATGATGTTTCATATTGTTGATTTGATCAGTTCGAGATTAATCGAAGCAATTGAA
AAAGATTTACAAAATTCAAGTGACCTTGAAATGAGAAATTGGGCACAAAGGTTTACAGCT
GATAATATTAGTAGCACTGCATTCGGCTTAGAAGATGAAAATTCTGAATTTTTAAAACAT
GGTAGAAGACTTTTTGATTTGACACCTTTTGAAACATTAAAATTTTTCTTCACAATTGGC
TGTCCAAATTTTTGTAGAAAATTAGGAGTCAGAACAAATCCAAAAGAACCAACAGATTTT
TTCCTAAAAACATTTTTGGAAACATTTGAATATCGCGAGAAAAACAATATTCAGAGAAAT
GATTTTGTTTCACTATTGCTTGGATTGAAAGAATTATTGACACCAATTGAATTAGCAGCT
GAGGCATTTTTGATGTTTGCAGCTGGATATGAAACAAGTTCAACATTGATGACTTTCATA
CTTTATGAGCTTGCACTTAATCCAGACATTCAACAAAAATTAAGAGATGAAATTGTTACA
AGAATTGAGGAAAATGATGGAAAATTAACTTATGAAATGTTGAATGAATTCAAATATCTT
GACATGGTGACTAATGAAACTTTAAGAAAATATCCTCCGATTCCGAATAATATTCGAATG
TGCACAAAAGATTACAAAATTGAAGAGCTTAATTTGACTATTCCTAAAGGAACAGCCATT
GAATTGCCAGTTTATTCACTTCAACACGATCCTGAATATTTTCCAAATCCAAACACATTT
GATCCTGAAAGATTTAACGATGAAAATGTCAAAAATATCAAACAATTTACATTCTTTCCA
TTTGGAGAAGGCAAAAGACAATGTTTAGGTTTAAGATTTGGTCTGATGCAAACCAAAATG
GGCATTGCAAAATTGTTAATAAATTATTCATTTTCATCTTGTGAAAAGACCATAATTCCA
ATGAAATTTGTACCAAGTGCTCCTTTTTTGCAACCAAAAGGTGGATTGTGGTTGAAAGTT
GAAAAAATTTTATAA

>g2249.t3 Gene=g2249 Length=464
MFVFVSLIFLFIISIYFWIKKIYSYWSEKGFLQNSNVKFPFGSLTGVGKKFTTCEALDII
YREIKGKSPVVGIYFFFKPTLMVIDPELIKKIFVQDFTYFQDRGFYHNKNSDPISANSGK
MKMMFHIVDLISSRLIEAIEKDLQNSSDLEMRNWAQRFTADNISSTAFGLEDENSEFLKH
GRRLFDLTPFETLKFFFTIGCPNFCRKLGVRTNPKEPTDFFLKTFLETFEYREKNNIQRN
DFVSLLLGLKELLTPIELAAEAFLMFAAGYETSSTLMTFILYELALNPDIQQKLRDEIVT
RIEENDGKLTYEMLNEFKYLDMVTNETLRKYPPIPNNIRMCTKDYKIEELNLTIPKGTAI
ELPVYSLQHDPEYFPNPNTFDPERFNDENVKNIKQFTFFPFGEGKRQCLGLRFGLMQTKM
GIAKLLINYSFSSCEKTIIPMKFVPSAPFLQPKGGLWLKVEKIL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g2249.t3 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 463 5.7E-112
2 g2249.t3 PANTHER PTHR24292 CYTOCHROME P450 4 117 4.0E-144
4 g2249.t3 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 4 117 4.0E-144
3 g2249.t3 PANTHER PTHR24292 CYTOCHROME P450 118 463 4.0E-144
5 g2249.t3 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 118 463 4.0E-144
8 g2249.t3 PRINTS PR00463 E-class P450 group I signature 257 274 2.2E-22
14 g2249.t3 PRINTS PR00385 P450 superfamily signature 268 285 3.4E-10
9 g2249.t3 PRINTS PR00463 E-class P450 group I signature 277 303 2.2E-22
6 g2249.t3 PRINTS PR00463 E-class P450 group I signature 321 339 2.2E-22
13 g2249.t3 PRINTS PR00385 P450 superfamily signature 322 333 3.4E-10
7 g2249.t3 PRINTS PR00463 E-class P450 group I signature 363 387 2.2E-22
11 g2249.t3 PRINTS PR00463 E-class P450 group I signature 398 408 2.2E-22
15 g2249.t3 PRINTS PR00385 P450 superfamily signature 399 408 3.4E-10
10 g2249.t3 PRINTS PR00463 E-class P450 group I signature 408 431 2.2E-22
12 g2249.t3 PRINTS PR00385 P450 superfamily signature 408 419 3.4E-10
1 g2249.t3 Pfam PF00067 Cytochrome P450 61 455 4.8E-80
19 g2249.t3 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
20 g2249.t3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
21 g2249.t3 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
22 g2249.t3 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 17 -
18 g2249.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 464 -
24 g2249.t3 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 401 410 -
16 g2249.t3 SUPERFAMILY SSF48264 Cytochrome P450 63 461 9.69E-101
23 g2249.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed