Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a14.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2249 g2249.t4 TSS g2249.t4 16276375 16276375
chr_3 g2249 g2249.t4 isoform g2249.t4 16276405 16278663
chr_3 g2249 g2249.t4 exon g2249.t4.exon1 16276405 16276754
chr_3 g2249 g2249.t4 cds g2249.t4.CDS1 16276405 16276754
chr_3 g2249 g2249.t4 exon g2249.t4.exon2 16277484 16277653
chr_3 g2249 g2249.t4 cds g2249.t4.CDS2 16277484 16277653
chr_3 g2249 g2249.t4 exon g2249.t4.exon3 16277711 16278010
chr_3 g2249 g2249.t4 cds g2249.t4.CDS3 16277711 16278010
chr_3 g2249 g2249.t4 exon g2249.t4.exon4 16278074 16278663
chr_3 g2249 g2249.t4 cds g2249.t4.CDS4 16278074 16278663
chr_3 g2249 g2249.t4 TTS g2249.t4 16278750 16278750

Sequences

>g2249.t4 Gene=g2249 Length=1410
ATGTTCGTCTTTGTTTCTTTAATATTTTTATTCATCATTTCAATTTATTTTTGGATAAAG
AAAATTTACAGCTATTGGAGTGAAAAGGGATTTTTACAAAATTCAAATGTGAAATTTCCA
TTTGGCTCTTTAACTGGTGTTGGTAAAAAATTTACAACTTGTGAAGCATTAGACATAATT
TACAGAGAAATTAAAGGAAAATCTCCTGTAGTTGGAATTTACTTCTTCTTTAAACCAACT
TTGATGGTCATCGACCCAGAGTTAATTAAGAAAATATTTGTTCAAGATTTTACATACTTT
CAAGATCGAGGATTTTATCACAACAAAAATTCTGACCCAATATCAGCAAACTCTGGAAAG
ATGAAAATGATGTTTCATATTGTTGATTTGATCAGTTCGAGATTAATCGAAGCAATTGAA
AAAGATTTACAAAATTCAAGTGACCTTGAAATGAGAAATTGGGCACAAAGGTTTACAGCT
GATAATATTAGTAGCACTGCATTCGGTTTAGATGCGAAATGCTTAGAAGATGAAAATTCT
GAATTTTTAAAACATGGTAGAAGACTTTTTGATTTGACACCTTTTGAAACATTAAAATTT
TTCTTCACAATTGGCTGTCCAAATTTTTGTAGAAAATTAGGAGTCAGAACAAATCCAAAA
GAACCAACAGATTTTTTCCTAAAAACATTTTTGGAAACATTTGAATATCGCGAGAAAAAC
AATATTCAGAGAAATGATTTTGTTTCACTATTGCTTGGATTGAAAGAATTATTGACACCA
ATTGAATTAGCAGCTGAGGCATTTTTGATGTTTGCAGCTGGATATGAAACAAGTTCAACA
TTGATGACTTTCATACTTTATGAGCTTGCACTTAATCCAGACATTCAACAAAAATTAAGA
GATGAAATTGTTACAAGAATTGAGGAAAATGATGGAAAATTAACTTATGAAATGTTGAAT
GAATTCAAATATCTTGACATGGTGACTAATGAAACTTTAAGAAAATATCCTCCGATTCCG
AATAATATTCGAATGTGCACAAAAGATTACAAAATTGAAGAGCTTAATTTGACTATTCCT
AAAGGAACAGCCATTGAATTGCCAGTTTATTCACTTCAACACGATCCTGAATATTTTCCA
AATCCAAACACATTTGATCCTGAAAGATTTAACGATGAAAATGTCAAAAATATCAAACAA
TTTACATTCTTTCCATTTGGAGAAGGCAAAAGACAATGTTTAGGTTTAAGATTTGGTCTG
ATGCAAACCAAAATGGGCATTGCAAAATTGTTAATAAATTATTCATTTTCATCTTGTGAA
AAGACCATAATTCCAATGAAATTTGTACCAAGTGCTCCTTTTTTGCAACCAAAAGGTGGA
TTGTGGTTGAAAGTTGAAAAAATTTTATAA

>g2249.t4 Gene=g2249 Length=469
MFVFVSLIFLFIISIYFWIKKIYSYWSEKGFLQNSNVKFPFGSLTGVGKKFTTCEALDII
YREIKGKSPVVGIYFFFKPTLMVIDPELIKKIFVQDFTYFQDRGFYHNKNSDPISANSGK
MKMMFHIVDLISSRLIEAIEKDLQNSSDLEMRNWAQRFTADNISSTAFGLDAKCLEDENS
EFLKHGRRLFDLTPFETLKFFFTIGCPNFCRKLGVRTNPKEPTDFFLKTFLETFEYREKN
NIQRNDFVSLLLGLKELLTPIELAAEAFLMFAAGYETSSTLMTFILYELALNPDIQQKLR
DEIVTRIEENDGKLTYEMLNEFKYLDMVTNETLRKYPPIPNNIRMCTKDYKIEELNLTIP
KGTAIELPVYSLQHDPEYFPNPNTFDPERFNDENVKNIKQFTFFPFGEGKRQCLGLRFGL
MQTKMGIAKLLINYSFSSCEKTIIPMKFVPSAPFLQPKGGLWLKVEKIL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g2249.t4 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 468 6.9E-116
3 g2249.t4 PANTHER PTHR24292 CYTOCHROME P450 4 117 4.9E-149
5 g2249.t4 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 4 117 4.9E-149
2 g2249.t4 PANTHER PTHR24292 CYTOCHROME P450 118 468 4.9E-149
4 g2249.t4 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 118 468 4.9E-149
8 g2249.t4 PRINTS PR00463 E-class P450 group I signature 262 279 2.3E-22
12 g2249.t4 PRINTS PR00385 P450 superfamily signature 273 290 3.6E-10
11 g2249.t4 PRINTS PR00463 E-class P450 group I signature 282 308 2.3E-22
6 g2249.t4 PRINTS PR00463 E-class P450 group I signature 326 344 2.3E-22
14 g2249.t4 PRINTS PR00385 P450 superfamily signature 327 338 3.6E-10
9 g2249.t4 PRINTS PR00463 E-class P450 group I signature 368 392 2.3E-22
10 g2249.t4 PRINTS PR00463 E-class P450 group I signature 403 413 2.3E-22
13 g2249.t4 PRINTS PR00385 P450 superfamily signature 404 413 3.6E-10
7 g2249.t4 PRINTS PR00463 E-class P450 group I signature 413 436 2.3E-22
15 g2249.t4 PRINTS PR00385 P450 superfamily signature 413 424 3.6E-10
1 g2249.t4 Pfam PF00067 Cytochrome P450 61 460 9.6E-83
19 g2249.t4 Phobius SIGNAL_PEPTIDE Signal peptide region 1 17 -
20 g2249.t4 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 1 -
21 g2249.t4 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 2 13 -
22 g2249.t4 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 17 -
18 g2249.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 18 469 -
24 g2249.t4 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 406 415 -
16 g2249.t4 SUPERFAMILY SSF48264 Cytochrome P450 63 466 6.16E-104
23 g2249.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values