Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock protein 83.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2254 g2254.t10 TSS g2254.t10 16306870 16306870
chr_3 g2254 g2254.t10 isoform g2254.t10 16306948 16308054
chr_3 g2254 g2254.t10 exon g2254.t10.exon1 16306948 16307013
chr_3 g2254 g2254.t10 exon g2254.t10.exon2 16307293 16307707
chr_3 g2254 g2254.t10 cds g2254.t10.CDS1 16307293 16307707
chr_3 g2254 g2254.t10 exon g2254.t10.exon3 16307777 16308054
chr_3 g2254 g2254.t10 cds g2254.t10.CDS2 16307777 16308054
chr_3 g2254 g2254.t10 TTS g2254.t10 16308052 16308052

Sequences

>g2254.t10 Gene=g2254 Length=759
TCAAAGCGATTAAAAGAATAAATAAGAGTTAATTATAGTCATAAAAGCAGAAAATCAAAA
AGAAAAATGCCAGCAGAAGCAGAAGTTTTCCAATTTCAAGCTGAAATAGCTCAGCTTATG
TCACTTATTATCAACACCTTTTATTCAAACAAGGAGATTTTCTTGCGTGAATTGATCTCA
AATTCATCTGATGCTCTCGATAAAATCCGTTATGAATCACTCACTGAACCTTCAAAATTG
GATTCAGGAAAGGAACTTTTTATTAAGATTATTCCAAACAAAGAGGCCCGTACTTTGACA
ATTATCGATACTGGTATTGGTATGACAAAGTCTGATCTCGTAAACAATCTTGGAACAATC
GCTCGCTCAGGCACAAAAGCCTTTATGGAAGCTTTACAAGCTGGCGCTGATATCTCTATG
ATTGGTCAATTTGGTGTCGGTTTCTATTCTGCATACTTGGTTGCTGACAAAGTCACAGTT
CTTGATGAAGGTGAGCCATTAGGTCGTGGTACAAAGATTGTTTTGCACATTAAAGAAGAT
CAGACTGAATACTTGGAAGAGTCAAAGATCAAGTCGATTGTCACAAAACACTCACAATTC
ATTGGCTATCCAATTAAATTGCTCGTTGAAAAAGAACGCGAGAAGGAAGTTAGTGATGAT
GAAGCTGAACCAGAAGAAGACAAAGAAAAGAAAGAAGGTGATGAGCCTAAGATTGAAGAT
GTTGGTGATGATGAAGATGCAGATAAGGCTGATAAGGAA

>g2254.t10 Gene=g2254 Length=231
MPAEAEVFQFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTEPSKLDS
GKELFIKIIPNKEARTLTIIDTGIGMTKSDLVNNLGTIARSGTKAFMEALQAGADISMIG
QFGVGFYSAYLVADKVTVLDEGEPLGRGTKIVLHIKEDQTEYLEESKIKSIVTKHSQFIG
YPIKLLVEKEREKEVSDDEAEPEEDKEKKEGDEPKIEDVGDDEDADKADKE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g2254.t10 CDD cd16927 HATPase_Hsp90-like 16 179 2.93049E-99
13 g2254.t10 Gene3D G3DSA:3.30.565.10 - 1 202 6.2E-77
17 g2254.t10 MobiDBLite mobidb-lite consensus disorder prediction 190 231 -
4 g2254.t10 PANTHER PTHR11528:SF79 HEAT SHOCK PROTEIN HSP 90-BETA-RELATED 4 139 2.7E-106
6 g2254.t10 PANTHER PTHR11528 HEAT SHOCK PROTEIN 90 FAMILY MEMBER 4 139 2.7E-106
3 g2254.t10 PANTHER PTHR11528:SF79 HEAT SHOCK PROTEIN HSP 90-BETA-RELATED 139 229 2.7E-106
5 g2254.t10 PANTHER PTHR11528 HEAT SHOCK PROTEIN 90 FAMILY MEMBER 139 229 2.7E-106
11 g2254.t10 PRINTS PR00775 90kDa heat shock protein signature 6 26 3.8E-62
9 g2254.t10 PRINTS PR00775 90kDa heat shock protein signature 27 49 3.8E-62
10 g2254.t10 PRINTS PR00775 90kDa heat shock protein signature 76 93 3.8E-62
7 g2254.t10 PRINTS PR00775 90kDa heat shock protein signature 94 111 3.8E-62
8 g2254.t10 PRINTS PR00775 90kDa heat shock protein signature 119 141 3.8E-62
2 g2254.t10 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase 28 158 3.8E-14
1 g2254.t10 Pfam PF00183 Hsp90 protein 161 229 3.5E-14
15 g2254.t10 ProSitePatterns PS00298 Heat shock hsp90 proteins family signature. 26 35 -
16 g2254.t10 SMART SM00387 HKATPase_4 28 159 7.8E-8
12 g2254.t10 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase 6 191 5.24E-65

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values