General information for g2254.t27
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heat shock protein 83.
Parent gene
- The parent gene g2254 is located on chr_3: 16308902..16314786 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2254 | g2254.t27 | isoform | g2254.t27 | 16308902 | 16309733 |
| chr_3 | g2254 | g2254.t27 | exon | g2254.t27.exon1 | 16308902 | 16309733 |
| chr_3 | g2254 | g2254.t27 | cds | g2254.t27.CDS1 | 16309018 | 16309500 |
| chr_3 | g2254 | g2254.t27 | TTS | g2254.t27 | 16309725 | 16309725 |
| chr_3 | g2254 | g2254.t27 | TSS | g2254.t27 | NA | NA |
Sequences
>g2254.t27 Gene=g2254 Length=832
ATTTAGGCAAACAACTTGTTTGTGTAACAAAGGAAGGTCTTGAATTGCCAGAAGATGAGG
AAGAGAAGAAGAAGCGTGAAGAAGATAAGGCCAAATTCGAAAATCTCTGCAAAGTCATGA
AATCAGTTTTAGATAACAAAGTTGAAAAAGTTATTGTATCAAATCGTTTGGTAGAATCAC
CATGCTGCATTGTCACATCTCAATATGGCTGGTCTGCTAATATGGAGCGTATCATGAAAG
CACAAGCTCTACGTGATACAACAACAATGGGTTACATGGCTGGTAAAAAACATCTTGAAA
TCAATCCAGACCATCCAATCATCGAGACATTGCGTCAAAAGGCTGAAGCCGACAAGAACG
ATAAGGCTGTTAAAGATTTGTGCATTTTACTCTTCGAGACTTCATTACTAAGCTCAGGTT
TTTCACTTGATGAACCACAAGTTCATGCTGCTAGAATTTATCGTATGATCAAACTCGGTT
TGGGAATTGATGAAGATGAACCAATGGCTACTGAAGAACCTGCTGCAGCAGCTTCATCAG
ATATGCCACCACTTGTAGATGATGCTGAAGATGCTTCACATATGGAAGAAGTTGATTAAA
TTGTTTTGCACTAATATCACTTAGTCTTTAAACAATTCCAAAAAATTTTTTGAATTTGAT
TTCCCAACTTTAATTTAAAATTTAAATTACGCTTGAAGATACACGACAGTTTCCTTTTAT
ATTTTTCATCATTCACTCAAACTTAAGTAATTTATAATTATAATAGATGGTACAATTTCA
AAGATTAATTTTGTTCTCATATTTCATTAGAAGCTAAGTTTTTGAAAAAAAA
>g2254.t27 Gene=g2254 Length=160
MKSVLDNKVEKVIVSNRLVESPCCIVTSQYGWSANMERIMKAQALRDTTTMGYMAGKKHL
EINPDHPIIETLRQKAEADKNDKAVKDLCILLFETSLLSSGFSLDEPQVHAARIYRMIKL
GLGIDEDEPMATEEPAAAASSDMPPLVDDAEDASHMEEVD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2254.t27 | Gene3D | G3DSA:1.20.120.790 | - | 1 | 122 | 7.0E-56 |
| 5 | g2254.t27 | MobiDBLite | mobidb-lite | consensus disorder prediction | 129 | 160 | - |
| 2 | g2254.t27 | PANTHER | PTHR11528:SF79 | HEAT SHOCK PROTEIN HSP 90-BETA-RELATED | 1 | 157 | 9.4E-74 |
| 3 | g2254.t27 | PANTHER | PTHR11528 | HEAT SHOCK PROTEIN 90 FAMILY MEMBER | 1 | 157 | 9.4E-74 |
| 1 | g2254.t27 | Pfam | PF00183 | Hsp90 protein | 1 | 135 | 2.3E-52 |
| 4 | g2254.t27 | SUPERFAMILY | SSF110942 | HSP90 C-terminal domain | 1 | 118 | 4.84E-38 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006457 | protein folding | BP |
| GO:0005524 | ATP binding | MF |
| GO:0051082 | unfolded protein binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
