General information for g2287.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine/threonine-protein kinase Chk2.
Parent gene
- The parent gene g2287 is located on chr_3: 16491580..16492403 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2287 | g2287.t3 | TSS | g2287.t3 | 16490345 | 16490345 |
| chr_3 | g2287 | g2287.t3 | isoform | g2287.t3 | 16490530 | 16492403 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon1 | 16490530 | 16490937 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon2 | 16490989 | 16491189 |
| chr_3 | g2287 | g2287.t3 | cds | g2287.t3.CDS1 | 16491018 | 16491189 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon3 | 16491247 | 16491512 |
| chr_3 | g2287 | g2287.t3 | cds | g2287.t3.CDS2 | 16491247 | 16491512 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon4 | 16491580 | 16491748 |
| chr_3 | g2287 | g2287.t3 | cds | g2287.t3.CDS3 | 16491580 | 16491748 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon5 | 16491803 | 16492047 |
| chr_3 | g2287 | g2287.t3 | cds | g2287.t3.CDS4 | 16491803 | 16492047 |
| chr_3 | g2287 | g2287.t3 | exon | g2287.t3.exon6 | 16492110 | 16492403 |
| chr_3 | g2287 | g2287.t3 | cds | g2287.t3.CDS5 | 16492110 | 16492403 |
| chr_3 | g2287 | g2287.t3 | TTS | g2287.t3 | 16492615 | 16492615 |
Sequences
>g2287.t3 Gene=g2287 Length=1583
ATGAATTCGCAAGATTTTGCAACCCAGGAATTTTCTAATAGTTCTCAATATTACACCGCT
CAAGTCGATGTTGAGAATACTCAGGTTTTGGCTCAGCATTGGGGAATGTTGAATATAATA
ACTAGTAACAAATGGATAGAATTAATTAAGGATAATGTACATATTGGAAGGTATGTGTTT
TTCTGTTCACGTGTTTTTCATCATTCTCTGAATTCTTTAAAATTTACAGAACTTTTTCTC
ATCCAAATGAAAGTGATCAATATGTGATTTTAGACAAACATGATCTTCCGCAATATAATG
TTTTTCAACGAGTTTCGAGTCATCATTTCACAATTCAGAAATTAAATAATGATGAAAGTA
ATGACTTTCCCTTTGGTGAAAATCAACCTGCTTTTATTATTTCTAAAGGTCAAAACGGTT
TGATTGTTGATGATGAAATGTTGAAAAAAGGCGAGAAAAAGATATTAAAAAATAATAGCC
TGATTAAGTTTCCATTTTACATGCATAAGACTTTTCAATTTAAATACTGGTGGAGTTTAA
ATGATCCTTCGATTACTTGCAGTTCAATACTTTTGAAAAATTATTTGGTATGTGGTATTA
TTGGACGAGGTTCTTGTGGTTCTGTTCGATTGGTAATTCCACTAAAGAAAATGCCAGAGG
AAATGAAGTCTAAAGCAGTGGCTTTGAAAACTATAGTTTACAACACATCAACTGTCTATA
ACAGTACAGCTCTTGATACTGTTAAAAACGAGGTTGATATTATGAAGAAACTATCCTATC
GACACGTTATGAGCTTGTTAAATCATTTCACTGCAGATGACAAGTATCTATATTTGCTTT
TTGATTTGATGGGTGGTGGAGATTTATTGAAACGCATTGTTGATTCTCCAGATAGACGTC
TTTCAGAAACGGACTCGAAATTTTTCTTTTTACAATTAGTTAAAGGCGTAAATTATTTGC
ACAGCAATCATGTCACTCATCGAGATATCAAAGCAGAAAATGTTTTTCTTTCCAATCGTG
GCATTTCACCTTTATTAAGAATCGGCGATTTTGGTTTGAGTAAGATCTCTGACGCGATGA
GTACTATTTGTGGTACTCTCTTATATACTGCGCCCGAAGTTTTCGAGTCATCTTTGTATT
CACAAAAAGTTGATATATGGAGTTTAGGTTGCTTGCTTTATGCAATGCTTTCAGGAAAAG
TCCCGTTCGCAGATCAAGGAAGTGGAAACGAAAAATTGCTCGAAGAAAAAATTAAAAAAG
CCGAATACAGTTTGAAAGGATTTAACCATGTCTCTTTAAGTTCAAAAAATCTCATAAATC
ACATGCTGCAAAAGAACCCTGCACTTCGTTTGGACACCACTCAAATCTTTAAACACGACT
GGTTAATTGATCAAACGAACTTTGAAAGACTTATGAAAACTTACCAAAATCATGGATTTC
CTGAAAATGAATGTCGTGAAGAATTGTTAATGGTGATGCAGCTTCAAAATTTGGCTATTA
CAGAAAATCGACAAAAACTATATGCTGATAACAATCTTCATTACAGCGATATGCAGGCTG
CAAAACGACAACGACTTCAATAA
>g2287.t3 Gene=g2287 Length=381
MLKKGEKKILKNNSLIKFPFYMHKTFQFKYWWSLNDPSITCSSILLKNYLVCGIIGRGSC
GSVRLVIPLKKMPEEMKSKAVALKTIVYNTSTVYNSTALDTVKNEVDIMKKLSYRHVMSL
LNHFTADDKYLYLLFDLMGGGDLLKRIVDSPDRRLSETDSKFFFLQLVKGVNYLHSNHVT
HRDIKAENVFLSNRGISPLLRIGDFGLSKISDAMSTICGTLLYTAPEVFESSLYSQKVDI
WSLGCLLYAMLSGKVPFADQGSGNEKLLEEKIKKAEYSLKGFNHVSLSSKNLINHMLQKN
PALRLDTTQIFKHDWLIDQTNFERLMKTYQNHGFPENECREELLMVMQLQNLAITENRQK
LYADNNLHYSDMQAAKRQRLQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2287.t3 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 41 | 381 | 1.5E-74 |
| 2 | g2287.t3 | PANTHER | PTHR44167 | OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED | 43 | 370 | 3.9E-66 |
| 1 | g2287.t3 | Pfam | PF00069 | Protein kinase domain | 53 | 316 | 6.8E-58 |
| 5 | g2287.t3 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 179 | 191 | - |
| 7 | g2287.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 49 | 316 | 44.725 |
| 4 | g2287.t3 | SMART | SM00220 | serkin_6 | 49 | 316 | 9.7E-81 |
| 3 | g2287.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 40 | 330 | 3.78E-71 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K06641 (serine/threonine-protein kinase CHEK2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
