General information for g2335.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase.
Parent gene
- The parent gene g2335 is located on chr_3: 16892172..16892882 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2335 | g2335.t3 | isoform | g2335.t3 | 16890648 | 16892882 |
| chr_3 | g2335 | g2335.t3 | exon | g2335.t3.exon1 | 16890648 | 16891285 |
| chr_3 | g2335 | g2335.t3 | cds | g2335.t3.CDS1 | 16890657 | 16891285 |
| chr_3 | g2335 | g2335.t3 | exon | g2335.t3.exon2 | 16891340 | 16891496 |
| chr_3 | g2335 | g2335.t3 | cds | g2335.t3.CDS2 | 16891340 | 16891496 |
| chr_3 | g2335 | g2335.t3 | exon | g2335.t3.exon3 | 16891592 | 16891842 |
| chr_3 | g2335 | g2335.t3 | cds | g2335.t3.CDS3 | 16891592 | 16891624 |
| chr_3 | g2335 | g2335.t3 | exon | g2335.t3.exon4 | 16891913 | 16892882 |
| chr_3 | g2335 | g2335.t3 | TTS | g2335.t3 | 16892890 | 16892890 |
| chr_3 | g2335 | g2335.t3 | TSS | g2335.t3 | NA | NA |
Sequences
>g2335.t3 Gene=g2335 Length=2016
GTTGCAGCTATGAAGCATTCTTGGATGGGCTATAAAACTTATGCCTTTGGTCATGACAAT
TTAAAGCCGATTAGTCAAACATATAGTGATTGGTTCAATTTGGGTCTTACGATAGTAGAT
GCTTTAGATACTCTTTATATAATGGATATGCAAGAAGAATACGGTGAAGGTAAACGCTGG
GTTGAATCAGAAATGAATTTGGACGTCAATCGTGATGTTAATTTATTTGAAACAACAATA
CGCGTGTTGGGTGGTTTATTATCTATGTATCATTTGTCTGGAGAAGAAATTTATCTAACT
AAAGCTAAAGATCTTGGAAGAAGATTGATGCCATCCTTTAATACACCATCAAAAATACCA
TTCTCAGATGTAAACTTGAATACTTTAAGTGCTCATGCTCCAAAATGGAGTCCTGATTCT
TCAACTTCAGAAGTAACAACTATTCAATTAGAATTTAGAGATTTGTCGCGTATATTAAAC
GATCCATCATTCGAAAATGCTGCTGCAATGGTGAGCCAAAAAATTCATGAGCTTCCAAAA
ACAGATGGACTTGTTCCGATCTTTATCAATGCGAATACAGGAACTTTTAGGACCTTTTCA
ACTATTTCATTAGGAGCTCGTGGAGATTCATATTACGAATATTTGTTAAAACAATGGCTT
CAAATCGGGAAACAACCTGATCATTATTTGATCGAAGACTACAAAGAAGCTATTGCTGGT
GTTATTAAAAATTTAAGAAAAGAAACACCAAATGAACATCATATCTACATTGGTGAATTA
ATAAGTGGAAAAGACTTATTTACCTGGAACGCTTTTACTTGGTTATAAAAACGGAATGCC
ACAATCTCATTTTACCTTGGCAACTGAATTGTTAGAAACATGTTATCAAACATATATAAA
GCAACCAACAGGATTAGCTCCAGAAATTACTTATTTCAATCTTGAGGGAGAAAGCTCAAC
TGACATCTATGTTAAAGCTAACGATGCTCACAATCTCCAAAGACCGGAATTCATTGAAAG
TCTTTATTATTTCTATGCAATTACAGGTAATAAAACTTACCAAGACATGGGATGGAATAT
TTTTCAATCGTTTGAAAAATATACTCGTGTAAGTCATGGTTACACGTCAATTAACAATGT
AAGAAATGCAAAGGATGTCCGTCCAAAAGACATGATGGAAACTTTTTGGCTCGGAGAAAC
TTTGAAATATTTCTATCTTTTGTTTAGTGACGATCGTAATGAAATTGATTTAGATAAATT
TATATTCAATACTGAAGCACATTTGATTCCTATTCGAGAAAACTGAAAATATGAACATTA
ACATTTATTAATTAACGAGAAAAAACGATATAAATTTTGGCATTCGTGCACTGAAAGTAG
GGGTATTGAAAAATTAAGAGAGGTTTTCTGTATGGTGTGTGTGAGAACGAGTCCATATGA
TAAGAGAAACATAATTGAATATACTAACGAAAGAAAAATTTTAACTTAATCATACATAAT
TAAATGTGATTGTAGGTATTAAAAATTAATTTATCATCTTTTGATTTTTTCGATAGCAAA
AAACAAGACAAAATGGCTACAATTTACCAAAATAGCAATTAAGTTTGTTTCTTTTCTAAT
ATGAATCCATATAAATCCTTATCAAATTTATGATATTAAAAAATTCTTGTTAATCAAAAA
ATATTAATTTCTTTTACAATACCATTAAATTGTAAATTTTATTAAGTAAAAAAATTTTCG
TCAACTTTATTGTCTAGTCTTAAACAGAAGAAAAGTTTCCCTTGTAAATAAATCATCATG
AGAGAAATTAATTGCATTTGCCTTTTTGATCAATATTGATTGAGATTTTTTATTATAATT
GATTGCGTATTTAATACATACATTAAATCTAAATAAAAATTTCTCTCGTAGTCATCAATA
ATTTATAAAATTATTTCTTATTAAAGTCTTCTAAGTACATTAGAGATTAAAAACAATGGT
AGGGAATGTGGAAATGATTCGATTAAAATAATTAAT
>g2335.t3 Gene=g2335 Length=272
MKHSWMGYKTYAFGHDNLKPISQTYSDWFNLGLTIVDALDTLYIMDMQEEYGEGKRWVES
EMNLDVNRDVNLFETTIRVLGGLLSMYHLSGEEIYLTKAKDLGRRLMPSFNTPSKIPFSD
VNLNTLSAHAPKWSPDSSTSEVTTIQLEFRDLSRILNDPSFENAAAMVSQKIHELPKTDG
LVPIFINANTGTFRTFSTISLGARGDSYYEYLLKQWLQIGKQPDHYLIEDYKEAIAGVIK
NLRKETPNEHHIYIGELISGKDLFTWNAFTWL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g2335.t3 | Gene3D | G3DSA:1.50.10.10 | - | 1 | 269 | 0 |
| 2 | g2335.t3 | PANTHER | PTHR11742 | MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED | 2 | 260 | 0 |
| 3 | g2335.t3 | PANTHER | PTHR11742:SF77 | ALPHA-1,2-MANNOSIDASE | 2 | 260 | 0 |
| 7 | g2335.t3 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 32 | 46 | 0 |
| 6 | g2335.t3 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 69 | 87 | 0 |
| 5 | g2335.t3 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 108 | 127 | 0 |
| 4 | g2335.t3 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 202 | 219 | 0 |
| 1 | g2335.t3 | Pfam | PF01532 | Glycosyl hydrolase family 47 | 1 | 258 | 0 |
| 8 | g2335.t3 | SUPERFAMILY | SSF48225 | Seven-hairpin glycosidases | 1 | 262 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005509 | calcium ion binding | MF |
| GO:0004571 | mannosyl-oligosaccharide 1,2-alpha-mannosidase activity | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
