Gene loci information

Transcript annotation

  • This transcript has been annotated as General transcription factor IIF subunit 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2413 g2413.t19 TTS g2413.t19 17778075 17778075
chr_3 g2413 g2413.t19 isoform g2413.t19 17778141 17778647
chr_3 g2413 g2413.t19 exon g2413.t19.exon1 17778141 17778647
chr_3 g2413 g2413.t19 cds g2413.t19.CDS1 17778141 17778638
chr_3 g2413 g2413.t19 TSS g2413.t19 17779135 17779135

Sequences

>g2413.t19 Gene=g2413 Length=507
AAAATTTATATGGAAGGTACAATAGTTGAGAAATTAGAATGTCGTCCTTATGCTGACACC
ACTTACATGAAACTTAAAGCAATGTCAATTAGAAATGCATCGCTTCCCAAACGTCGTGTT
GAACAATTAGATACTGTTGTGACTACATTTAAACCAGTCTCTGACCATAAACATAATATC
GAGTATGAGAATAAGAAAAAGAACGATGGGAAGAAATCTCGTGCAGATAAAAATGCTGTT
ATGGATGTCTTATTTAATGCATTCGAAAAACATCAGTATTACAATATCAAAGATCTTGTT
ACAATCACAAATCAACCAATTTCATACTTGAAAGAAATTCTCAAAGAAATATGCGAATAT
AGTGTGAAGAATCCTCATAAAAATACATGGCATTTGAAAGCAGAATATAGGCATTATTCT
GAAGAGAAAAAGGATAAAAAGGCTGATACTAAAGATGAAGGAACTGATGACAAAATGAGC
GATAGCGATGATGACGATGACTTTTAG

>g2413.t19 Gene=g2413 Length=165
MEGTIVEKLECRPYADTTYMKLKAMSIRNASLPKRRVEQLDTVVTTFKPVSDHKHNIEYE
NKKKNDGKKSRADKNAVMDVLFNAFEKHQYYNIKDLVTITNQPISYLKEILKEICEYSVK
NPHKNTWHLKAEYRHYSEEKKDKKADTKDEGTDDKMSDSDDDDDF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g2413.t19 Gene3D G3DSA:1.10.10.10 winged helix repressor DNA binding domain 69 137 1.3E-31
5 g2413.t19 MobiDBLite mobidb-lite consensus disorder prediction 137 154 -
6 g2413.t19 MobiDBLite mobidb-lite consensus disorder prediction 137 165 -
2 g2413.t19 PANTHER PTHR10445:SF0 GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2 1 145 1.2E-44
3 g2413.t19 PANTHER PTHR10445 GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2 1 145 1.2E-44
1 g2413.t19 Pfam PF02270 TFIIF, beta subunit HTH domain 71 134 2.2E-23
4 g2413.t19 SUPERFAMILY SSF46785 Winged helix DNA-binding domain 71 136 5.98E-23

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005674 transcription factor TFIIF complex CC
GO:0006367 transcription initiation from RNA polymerase II promoter BP
GO:0006366 transcription by RNA polymerase II BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values