General information for g2468.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Eukaryotic translation initiation factor 2-alpha kinase 1.
Parent gene
- The parent gene g2468 is located on chr_3: 18118060..18119357 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2468 | g2468.t1 | TSS | g2468.t1 | 18117434 | 18117434 |
| chr_3 | g2468 | g2468.t1 | isoform | g2468.t1 | 18117506 | 18119357 |
| chr_3 | g2468 | g2468.t1 | exon | g2468.t1.exon1 | 18117506 | 18117584 |
| chr_3 | g2468 | g2468.t1 | cds | g2468.t1.CDS1 | 18117506 | 18117584 |
| chr_3 | g2468 | g2468.t1 | exon | g2468.t1.exon2 | 18117657 | 18118787 |
| chr_3 | g2468 | g2468.t1 | cds | g2468.t1.CDS2 | 18117657 | 18118787 |
| chr_3 | g2468 | g2468.t1 | exon | g2468.t1.exon3 | 18118852 | 18119357 |
| chr_3 | g2468 | g2468.t1 | cds | g2468.t1.CDS3 | 18118852 | 18119357 |
| chr_3 | g2468 | g2468.t1 | TTS | g2468.t1 | 18119507 | 18119507 |
Sequences
>g2468.t1 Gene=g2468 Length=1716
ATGACATCCAGTGATAGTGAAAGTGAAGTGGAAAACTCATTAGCTTGGTCTAAGCTTGAG
ACAAATAAGGAATTTGATGTAAAATCAAATCAAAAGATGATTGTGAAGAGAAATTCATCA
ACAGATGAGATTGATACTGGAAGAAACAATCGATTATTTAATTTAACACCATCAATATCA
TGTAGTCTTCTTGTTCAAAGTTTGTTAAAACAATTGTGTTCTTTTATTGAAACGGATCAA
GTAAAATCTTCTGATATGTACGCTAAAATTTGTAAGAAGCTTCATGAATTTAGAATGATT
GATGAGAGTTATGAGAGAAGTGAGTTTGAAAACATTCGAGCATCATACGAAGCTGCAATC
ACTGCATTAGTAAATGTGACGAAGGAAACATCTCAAAACAATGAAATGAATTTAGTGGAA
TGGCCTCTCAAGAATGATAAAAGAGCATTAGAATGGAGCAGATATCATCGAGATTTTATA
GAACTTGAGAAAATATCCAGTGGCGGTTTTGGTGATGTTTACAAAGCGATGCACAATCTT
GATAATAATATTTATGCGATAAAGAAAATTCTCATCAAATCTACATCAGTTAAGAACATT
CTTTCTCATTTAAGAGAAGTTAAAACTTTTGCTTCTCTAAATCATATTAATGTGGTACCT
TACAAGAGTTGCTGGCTAGAACCTTTGATTTCATATGAACAAGAAAAGACAATATACGAT
GATGAAAGTTGGACAACAAAAAGTGAAAGTACTACCAATATAAGAATAACGAAAAGCAAT
GACAATTCATTACGCCTTCAAACCAACGACAGTTTTAGTATTAACTTTGAATACAGTGAA
AACCATCAAAGTGAGCAAGTAAAAAGTGCAGATTCTTCAATTATGACAAATGGAGAAAGT
CATTCTCAAGTGGAACGACTTAGAAAACTCAATTTATCAAAATCAAATGGAAGTACTTAT
TCTTTAGCAATTGTTCCTCATGTAAAATTGGCATGGTCTGTTTTATACATCCAAATGAAG
CTATGTCAAAAGACGCTGAGAAACTTTTTAGATGAGCGAAATGAACATGAATGCTTCAAA
AAGTATTATGAGAATGTTAATCTTCGTAGTGATAATCTTACTTACACAGCCTTAAGTATT
TTTAAGCAAATATGCAATGGATTAGAGTACATACATAGTAAATCAATAGTTCATCATGAT
CTTAAGCCTGCAAATGTTTTTGTGTCATTTGAGAAATCTAATAATAAAGTAACATTCTCT
ATAGGAGATTTTGGACTAGCTTGTCCACTCGATAATAATGAGATGCTATCACACAATGGT
ATTGGAACGAGGCTTTATGCTGCGAAAGAGCAATTTGAAGGTCGAATTTGTTCAAAAAAG
TCGGACATTTATAGTTTAGGTGTCATTCTAATTGAACTTCTTTTACCTTGCAAAACAGTC
ATGGAATGTTTCAAGAAAGTAGAAAAAATCAAAAAAGGCGAATTATTACCAGAAATAGAA
GCCAACTTGTGTAAACTTATTGGCAGACTTCTGAGTCAACGAGCAGAATTGAGACCAGAT
GTGAGCGAATTAAAAAGCTTAATACGTCATTTTATGGACAACACATCAGATGAAGTTGCG
CGTTTAAAAAATATTATCGAAGATAAAGATAAGCAAATTGATGATTTGATGCAAGAGATT
AGGAATTTAAAGAAACAGTTGTCAAAAGAAACATGA
>g2468.t1 Gene=g2468 Length=571
MTSSDSESEVENSLAWSKLETNKEFDVKSNQKMIVKRNSSTDEIDTGRNNRLFNLTPSIS
CSLLVQSLLKQLCSFIETDQVKSSDMYAKICKKLHEFRMIDESYERSEFENIRASYEAAI
TALVNVTKETSQNNEMNLVEWPLKNDKRALEWSRYHRDFIELEKISSGGFGDVYKAMHNL
DNNIYAIKKILIKSTSVKNILSHLREVKTFASLNHINVVPYKSCWLEPLISYEQEKTIYD
DESWTTKSESTTNIRITKSNDNSLRLQTNDSFSINFEYSENHQSEQVKSADSSIMTNGES
HSQVERLRKLNLSKSNGSTYSLAIVPHVKLAWSVLYIQMKLCQKTLRNFLDERNEHECFK
KYYENVNLRSDNLTYTALSIFKQICNGLEYIHSKSIVHHDLKPANVFVSFEKSNNKVTFS
IGDFGLACPLDNNEMLSHNGIGTRLYAAKEQFEGRICSKKSDIYSLGVILIELLLPCKTV
MECFKKVEKIKKGELLPEIEANLCKLIGRLLSQRAELRPDVSELKSLIRHFMDNTSDEVA
RLKNIIEDKDKQIDDLMQEIRNLKKQLSKET
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g2468.t1 | Coils | Coil | Coil | 532 | 571 | - |
| 5 | g2468.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 147 | 269 | 4.2E-26 |
| 6 | g2468.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 374 | 567 | 9.7E-39 |
| 3 | g2468.t1 | PANTHER | PTHR11042 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED | 29 | 528 | 7.4E-78 |
| 1 | g2468.t1 | Pfam | PF00069 | Protein kinase domain | 161 | 227 | 1.4E-9 |
| 2 | g2468.t1 | Pfam | PF00069 | Protein kinase domain | 335 | 525 | 9.3E-27 |
| 8 | g2468.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 396 | 408 | - |
| 10 | g2468.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 159 | 532 | 31.397 |
| 9 | g2468.t1 | SMART | SM00220 | serkin_6 | 159 | 532 | 3.2E-19 |
| 4 | g2468.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 150 | 526 | 9.29E-55 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K16194 (eukaryotic translation initiation factor 2-alpha kinase 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
