General information for g2468.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Eukaryotic translation initiation factor 2-alpha kinase 1.
Parent gene
- The parent gene g2468 is located on chr_3: 18118060..18119357 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2468 | g2468.t3 | TSS | g2468.t3 | 18117434 | 18117434 |
| chr_3 | g2468 | g2468.t3 | isoform | g2468.t3 | 18117506 | 18118361 |
| chr_3 | g2468 | g2468.t3 | exon | g2468.t3.exon1 | 18117506 | 18117584 |
| chr_3 | g2468 | g2468.t3 | cds | g2468.t3.CDS1 | 18117506 | 18117584 |
| chr_3 | g2468 | g2468.t3 | exon | g2468.t3.exon2 | 18117657 | 18118361 |
| chr_3 | g2468 | g2468.t3 | cds | g2468.t3.CDS2 | 18117657 | 18118360 |
| chr_3 | g2468 | g2468.t3 | TTS | g2468.t3 | NA | NA |
Sequences
>g2468.t3 Gene=g2468 Length=784
ATGACATCCAGTGATAGTGAAAGTGAAGTGGAAAACTCATTAGCTTGGTCTAAGCTTGAG
ACAAATAAGGAATTTGATGTAAAATCAAATCAAAAGATGATTGTGAAGAGAAATTCATCA
ACAGATGAGATTGATACTGGAAGAAACAATCGATTATTTAATTTAACACCATCAATATCA
TGTAGTCTTCTTGTTCAAAGTTTGTTAAAACAATTGTGTTCTTTTATTGAAACGGATCAA
GTAAAATCTTCTGATATGTACGCTAAAATTTGTAAGAAGCTTCATGAATTTAGAATGATT
GATGAGAGTTATGAGAGAAGTGAGTTTGAAAACATTCGAGCATCATACGAAGCTGCAATC
ACTGCATTAGTAAATGTGACGAAGGAAACATCTCAAAACAATGAAATGAATTTAGTGGAA
TGGCCTCTCAAGAATGATAAAAGAGCATTAGAATGGAGCAGATATCATCGAGATTTTATA
GAACTTGAGAAAATATCCAGTGGCGGTTTTGGTGATGTTTACAAAGCGATGCACAATCTT
GATAATAATATTTATGCGATAAAGAAAATTCTCATCAAATCTACATCAGTTAAGAACATT
CTTTCTCATTTAAGAGAAGTTAAAACTTTTGCTTCTCTAAATCATATTAATGTGGTACCT
TACAAGAGTTGCTGGCTAGAACCTTTGATTTCATATGAACAAGAAAAGACAATATACGAT
GATGAAAGTTGGACAACAAAAAGTGAAAGTACTACCAATATAAGAATAACGAAAAGCAAT
GACA
>g2468.t3 Gene=g2468 Length=261
MTSSDSESEVENSLAWSKLETNKEFDVKSNQKMIVKRNSSTDEIDTGRNNRLFNLTPSIS
CSLLVQSLLKQLCSFIETDQVKSSDMYAKICKKLHEFRMIDESYERSEFENIRASYEAAI
TALVNVTKETSQNNEMNLVEWPLKNDKRALEWSRYHRDFIELEKISSGGFGDVYKAMHNL
DNNIYAIKKILIKSTSVKNILSHLREVKTFASLNHINVVPYKSCWLEPLISYEQEKTIYD
DESWTTKSESTTNIRITKSND
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g2468.t3 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 146 | 260 | 0.000 |
| 2 | g2468.t3 | PANTHER | PTHR11042 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED | 26 | 240 | 0.000 |
| 1 | g2468.t3 | Pfam | PF00069 | Protein kinase domain | 161 | 227 | 0.000 |
| 5 | g2468.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 159 | 261 | 10.478 |
| 3 | g2468.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 133 | 228 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
