General information for g2494.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphoribosyl pyrophosphate synthase-associated protein 2.
Parent gene
- The parent gene g2494 is located on chr_3: 18280776..18288527 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2494 | g2494.t5 | isoform | g2494.t5 | 18279650 | 18281389 |
| chr_3 | g2494 | g2494.t5 | exon | g2494.t5.exon1 | 18279650 | 18279770 |
| chr_3 | g2494 | g2494.t5 | exon | g2494.t5.exon2 | 18279891 | 18280221 |
| chr_3 | g2494 | g2494.t5 | cds | g2494.t5.CDS1 | 18280145 | 18280221 |
| chr_3 | g2494 | g2494.t5 | exon | g2494.t5.exon3 | 18280283 | 18280402 |
| chr_3 | g2494 | g2494.t5 | cds | g2494.t5.CDS2 | 18280283 | 18280402 |
| chr_3 | g2494 | g2494.t5 | exon | g2494.t5.exon4 | 18280463 | 18280510 |
| chr_3 | g2494 | g2494.t5 | cds | g2494.t5.CDS3 | 18280463 | 18280510 |
| chr_3 | g2494 | g2494.t5 | exon | g2494.t5.exon5 | 18280567 | 18281389 |
| chr_3 | g2494 | g2494.t5 | cds | g2494.t5.CDS4 | 18280567 | 18281389 |
| chr_3 | g2494 | g2494.t5 | TTS | g2494.t5 | 18281730 | 18281730 |
| chr_3 | g2494 | g2494.t5 | TSS | g2494.t5 | NA | NA |
Sequences
>g2494.t5 Gene=g2494 Length=1443
ATGAACAGAAAATCGTAAGTTGGCCTAATTTAATTAATATTTCATCGGTTTTCATATATT
TGCCAGTGTTTCAATTGCTTCAATCTTGTTATAACGTGATTGTTTATCGAAAAATGTCAA
ATTAAAAACCCTTCACTAACTGTTTGCTTGATCACTAGTATCCCAATTTTTCGAAAGTCG
TACATTTGTATTGTCAATGCGCTTCGTCAAAAAAACATAAACATAACACAAATCCATTAC
ATTAGCACGCACAAAATAAAATTTTCGACTGTATAAACAGTTTGATCGATAAGAAATCAC
TCTTCATTCTTGCAATCAATCAACTTGTTCGAATTTTATAGATTTCAACCCGTAAGATCG
TTATCAAACCACTATATGGATTGTCCGTCAACGTCCGACATTGTGCTTCTGCACGGCAAT
TCTTGTCCAGAGTTAGCAAACCTTATTGGCAGCCGATTAAATATTAAGTCTGGAGGATGC
TCAGTTTATCACAAGCCAAATCGAGAAACAATGGTTGATATCGGTGACTCTGTGCGCGGA
AAAGATTGCTATATCATACAAACTGGAACAAAGGATTGCAACAACAATATTATGGAATTA
TTGATAATGGCATATGCTTGTAAAACATCAAGCGCAAAATCTATTGTTGCTGTTATTCCT
TACTTGCCCTATTCAAAGCAATGTAAGATGCGTAAACGTGGATCAATTGTCACAAAACTC
ATTGCTAAAATGATGTGTAATGCTGGATTAACTCATGTTATTACAATGGACTTACATCAG
AAGGAAATTCAAGGATTTTTTGATTGTCCTGTTGATAATCTTCGAGCTTCGCCATTTTTA
CTTCAATATATTCAAGAGTCAATTCCTGATTATCGTAATGCTGTTATTGTCGCTCGTAAT
CCTGGATCGGCTAAAAAAGCTACCTCTTATGCTGAACGTTTACGTTTAGGAATCGCTGTT
ATCCATGGTGAACAAAAAGATGCTGATAATGATGAAATTGACGGTCGAAATTCGCCACCA
AATGTTCCAAAAGGACGAATGAGTGATATTTCATCAGGATTTCCAAGTCTCGTTGCTAAA
GAAAAGCCACCGATTAATGTGGTTGGTGATGTGGGAGGCAGAATTGCTGTAATTGTTGAT
GATTTAATAGATGAAGTACAATCATTTGTCTCGGCTGCTGAAGTTCTTAAAGAAAGAGGA
GCTTATAAAATTTATGTTTTGGCAACACATGGTTTGCTTTCTTCTGATGCACCACGCTTA
ATTGAAGAATCTGCTATTGATGAAGTTGTCGTTACAAATACTATACCGCATGAAATTCAG
AAAATGCAGTGCCATAAAATTAAAACAGTCGATATTTCGATTCTTTTGTCAGAAGCTATT
CGAAGAATACATAACAAAGAATCGATGTCTCATCTTTTCAGAAATGTTACACTTGAGGAC
TAA
>g2494.t5 Gene=g2494 Length=355
MDCPSTSDIVLLHGNSCPELANLIGSRLNIKSGGCSVYHKPNRETMVDIGDSVRGKDCYI
IQTGTKDCNNNIMELLIMAYACKTSSAKSIVAVIPYLPYSKQCKMRKRGSIVTKLIAKMM
CNAGLTHVITMDLHQKEIQGFFDCPVDNLRASPFLLQYIQESIPDYRNAVIVARNPGSAK
KATSYAERLRLGIAVIHGEQKDADNDEIDGRNSPPNVPKGRMSDISSGFPSLVAKEKPPI
NVVGDVGGRIAVIVDDLIDEVQSFVSAAEVLKERGAYKIYVLATHGLLSSDAPRLIEESA
IDEVVVTNTIPHEIQKMQCHKIKTVDISILLSEAIRRIHNKESMSHLFRNVTLED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g2494.t5 | CDD | cd06223 | PRTases_typeI | 151 | 309 | 1.7856E-16 |
| 8 | g2494.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 202 | 222 | - |
| 3 | g2494.t5 | PANTHER | PTHR10210:SF53 | GH23275P | 5 | 351 | 1.4E-171 |
| 4 | g2494.t5 | PANTHER | PTHR10210 | RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER | 5 | 351 | 1.4E-171 |
| 2 | g2494.t5 | Pfam | PF13793 | N-terminal domain of ribose phosphate pyrophosphokinase | 10 | 124 | 1.6E-37 |
| 1 | g2494.t5 | Pfam | PF14572 | Phosphoribosyl synthetase-associated domain | 165 | 349 | 2.4E-99 |
| 7 | g2494.t5 | SMART | SM01400 | Pribosyltran_N_2 | 9 | 124 | 6.3E-49 |
| 6 | g2494.t5 | SUPERFAMILY | SSF53271 | PRTase-like | 8 | 161 | 1.02E-44 |
| 5 | g2494.t5 | SUPERFAMILY | SSF53271 | PRTase-like | 165 | 348 | 9.96E-43 |
| 9 | g2494.t5 | TIGRFAM | TIGR01251 | ribP_PPkin: ribose-phosphate diphosphokinase | 10 | 349 | 2.6E-82 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000287 | magnesium ion binding | MF |
| GO:0004749 | ribose phosphate diphosphokinase activity | MF |
| GO:0009116 | nucleoside metabolic process | BP |
| GO:0009165 | nucleotide biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
