General information for g2508.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein-S-isoprenylcysteine O-methyltransferase.
Parent gene
- The parent gene g2508 is located on chr_3: 18335879..18337750 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2508 | g2508.t10 | isoform | g2508.t10 | 18335460 | 18337010 |
| chr_3 | g2508 | g2508.t10 | exon | g2508.t10.exon1 | 18335460 | 18335816 |
| chr_3 | g2508 | g2508.t10 | cds | g2508.t10.CDS1 | 18335613 | 18335816 |
| chr_3 | g2508 | g2508.t10 | exon | g2508.t10.exon2 | 18335881 | 18336331 |
| chr_3 | g2508 | g2508.t10 | cds | g2508.t10.CDS2 | 18335881 | 18336331 |
| chr_3 | g2508 | g2508.t10 | exon | g2508.t10.exon3 | 18336803 | 18337010 |
| chr_3 | g2508 | g2508.t10 | cds | g2508.t10.CDS3 | 18336803 | 18336831 |
| chr_3 | g2508 | g2508.t10 | TSS | g2508.t10 | NA | NA |
| chr_3 | g2508 | g2508.t10 | TTS | g2508.t10 | NA | NA |
Sequences
>g2508.t10 Gene=g2508 Length=1016
AGTAAATTTGAATAAAAGAAAAGTTAGAAAATAATAGAATTTTTTTACTGAGTTTGTCTC
CCTCTGATTAAAATTTACAAGTGAAATAATTCTATCGGCGTATCAAATTTTTTAAATAAA
GAATACTAAAAGTAAAGGGTTTCATTTATTACTATGATATGCCATGAAGGAAAAGTTAGT
GTCATAGCATTCATTACAAGCTTGCTAGTGCAATTGTTTGGTGTATCAGAATTCGGAGAA
AAACTTGCTCTATTCATTAGTGAATCATTTTTTTCATCAATTTACACTCTTTTTGTTCTG
TATTTTATCGTGCTGATATTATTCTTATCAATATGTTTCAAATCAACAGACTTTCAGATT
GCTATTCGAGCTGGAATTTTAGGGTATTTTTTTGGCATTGCTCTTTTTATTTCAATTACA
ATGGAGGATAAATACAAGTCTTTTGGAATTTATTTGGCTTTTTTAACATTCTTTCATTAC
TCGGAGTATTTTGTAATTTCGTTAAGTCATCCAGAATCATTGACACTTGATTCTTTTATG
CTTAATCATTCAATTCAATATGAATTTGCGGCTATAATTAGTTGGATAGAATATTTCGTT
GAAGTTTATTATTATCCTGAAATGAAAAAGTGCAAAACAATTTGGATAATAGGTTCTACT
ATTTGCTTAAGTGGTGAAATTTTGAGAAAGGTTGCAATGTTAACAGCCAAAAAAAGTTTC
CATCATTTAGTGCAATTTCAGCAAGCCGATGATCATAAATTAATAACGATAGGAGTTTAT
AGTTGGTTTCGACATCCTGCTTATGTGACAATTAATCTTTCTTTTTTTTTCAGCTAATAA
TGGCAAACCCAATTTGTTTTGTTCTCTATCTCATTGCAAGCTGGCTTTTTTTCAAAGAGA
GAATCCATACTGAAGAGATCACTTTACTCAATTTCTTTGGACAAGAATATGTTCAATATC
AGCAAAGAACAAAGACTGGTTTACCATTCATAAATGGATATATTACAACAAACTAA
>g2508.t10 Gene=g2508 Length=227
MICHEGKVSVIAFITSLLVQLFGVSEFGEKLALFISESFFSSIYTLFVLYFIVLILFLSI
CFKSTDFQIAIRAGILGYFFGIALFISITMEDKYKSFGIYLAFLTFFHYSEYFVISLSHP
ESLTLDSFMLNHSIQYEFAAIISWIEYFVEVYYYPEMKKCKTIWIIGSTICLSGEILRKV
AMLTAKKSFHHLVQFQQADDHKLITIGVYSWFRHPAYVTINLSFFFS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g2508.t10 | Gene3D | G3DSA:1.20.120.1630 | - | 98 | 227 | 5.3E-11 |
| 2 | g2508.t10 | PANTHER | PTHR12714:SF9 | PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE | 75 | 218 | 2.6E-30 |
| 3 | g2508.t10 | PANTHER | PTHR12714 | PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE | 75 | 218 | 2.6E-30 |
| 1 | g2508.t10 | Pfam | PF04140 | Isoprenylcysteine carboxyl methyltransferase (ICMT) family | 170 | 219 | 3.4E-12 |
| 17 | g2508.t10 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 18 | g2508.t10 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 19 | g2508.t10 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 19 | - |
| 25 | g2508.t10 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 20 | 23 | - |
| 14 | g2508.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 38 | - |
| 24 | g2508.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 39 | 62 | - |
| 13 | g2508.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 63 | 68 | - |
| 23 | g2508.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 69 | 90 | - |
| 15 | g2508.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 91 | 95 | - |
| 21 | g2508.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 115 | - |
| 11 | g2508.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 116 | 135 | - |
| 22 | g2508.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 136 | 155 | - |
| 16 | g2508.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 156 | 160 | - |
| 20 | g2508.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 177 | - |
| 12 | g2508.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 178 | 227 | - |
| 10 | g2508.t10 | ProSiteProfiles | PS51564 | Protein-S-isoprenylcysteine O-methyltransferase (EC 2.1.1.100) family profile. | 9 | 218 | 15.833 |
| 7 | g2508.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 10 | 27 | - |
| 6 | g2508.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 40 | 62 | - |
| 4 | g2508.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 67 | 89 | - |
| 5 | g2508.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 118 | - |
| 8 | g2508.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 133 | 155 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0016021 | integral component of membrane | CC |
| GO:0006481 | C-terminal protein methylation | BP |
| GO:0004671 | protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed