Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Methionyl-tRNA formyltransferase, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2508 g2508.t4 TSS g2508.t4 18333862 18333862
chr_3 g2508 g2508.t4 isoform g2508.t4 18334439 18335460
chr_3 g2508 g2508.t4 exon g2508.t4.exon1 18334439 18334560
chr_3 g2508 g2508.t4 exon g2508.t4.exon2 18334616 18334649
chr_3 g2508 g2508.t4 exon g2508.t4.exon3 18334834 18335460
chr_3 g2508 g2508.t4 cds g2508.t4.CDS1 18334836 18335459
chr_3 g2508 g2508.t4 TTS g2508.t4 18335491 18335491

Sequences

>g2508.t4 Gene=g2508 Length=783
ATGATTTTTAGGAATTTTTGTCATAAAATAAAATGGAAATATTTAGAATTATCTCGTAAT
TATTCACATAAAATTTTATTTTTCGGAACAGATAATTTCTCACTTCCATCATTAAAATTA
ATAACTAAACAAAATGATCTATATTCACGTCTTGAGGGATGCTAAATGTACATGCAAGTC
TACTGCCAAAATATCGAGGTGCGAGTCCTATAATTTACGCTATAAAAAATCATGAAACTA
TAACAGGTGTATCAATAATGAAAATCAAGGCAAAAAAGTTTGATGTAGGGGATGTACTTG
CGATGAAAGAAGTATCTATAACAGATGACATTCTTATGTCAGAATTACACGACCAATTAG
CTAATTTAGGAGCACAATTATTAATCGATTGTCTCAAACATTTAGAAGAATGTAAGCCAA
TAAAGCAAGACGAGAGCCAAGCATCATACGCACCTAAAATTGATAAAGAATTTTGCAAAG
TAAGATGGAATGAAATGAGTGCACGTGAGATTTATGATTTATACAGAGCACTCTATTCTT
ACAAGCATGTGTTGACAACATTTAAAAATGAACCGGTTAAGTTGTTAGAATTAAAAAAAT
CTAATGAAGTCATTGAATATAACATTGCTGGACATTTAAAATTTTGTAAAAAAACGAAAA
AACTATTAGTGCATTGTGTTGATGGTGAATGTATAGAAATTAATAAATTACTGATAGCTA
AAAAAGTGATGAGTGCATCAGATTTTAATAATGGATTCTTAAAAAACATAAATGATTTTG
AAA

>g2508.t4 Gene=g2508 Length=208
MLNVHASLLPKYRGASPIIYAIKNHETITGVSIMKIKAKKFDVGDVLAMKEVSITDDILM
SELHDQLANLGAQLLIDCLKHLEECKPIKQDESQASYAPKIDKEFCKVRWNEMSAREIYD
LYRALYSYKHVLTTFKNEPVKLLELKKSNEVIEYNIAGHLKFCKKTKKLLVHCVDGECIE
INKLLIAKKVMSASDFNNGFLKNINDFE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g2508.t4 CDD cd08704 Met_tRNA_FMT_C 104 193 0
7 g2508.t4 Gene3D G3DSA:3.40.50.12230 - 1 203 0
3 g2508.t4 PANTHER PTHR11138 METHIONYL-TRNA FORMYLTRANSFERASE 1 204 0
4 g2508.t4 PANTHER PTHR11138:SF0 METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL 1 204 0
1 g2508.t4 Pfam PF00551 Formyl transferase 1 77 0
2 g2508.t4 Pfam PF02911 Formyl transferase, C-terminal domain 100 200 0
5 g2508.t4 SUPERFAMILY SSF53328 Formyltransferase 1 103 0
6 g2508.t4 SUPERFAMILY SSF50486 FMT C-terminal domain-like 101 200 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0009058 biosynthetic process BP
GO:0016742 hydroxymethyl-, formyl- and related transferase activity MF
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values