General information for g2513.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4c3.
Parent gene
- The parent gene g2513 is located on chr_3: 18354554..18362744 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2513 | g2513.t1 | TSS | g2513.t1 | 18354398 | 18354398 |
| chr_3 | g2513 | g2513.t1 | isoform | g2513.t1 | 18354554 | 18362744 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon1 | 18354554 | 18354752 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS1 | 18354554 | 18354752 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon2 | 18354831 | 18354863 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS2 | 18354831 | 18354863 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon3 | 18358702 | 18358817 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS3 | 18358702 | 18358817 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon4 | 18360067 | 18360183 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS4 | 18360067 | 18360183 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon5 | 18360250 | 18360409 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS5 | 18360250 | 18360409 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon6 | 18360505 | 18360574 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS6 | 18360505 | 18360574 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon7 | 18360649 | 18360986 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS7 | 18360649 | 18360986 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon8 | 18361836 | 18361856 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS8 | 18361836 | 18361856 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon9 | 18362005 | 18362180 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS9 | 18362005 | 18362180 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon10 | 18362236 | 18362278 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS10 | 18362236 | 18362278 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon11 | 18362342 | 18362510 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS11 | 18362342 | 18362510 |
| chr_3 | g2513 | g2513.t1 | exon | g2513.t1.exon12 | 18362573 | 18362744 |
| chr_3 | g2513 | g2513.t1 | cds | g2513.t1.CDS12 | 18362573 | 18362744 |
| chr_3 | g2513 | g2513.t1 | TTS | g2513.t1 | 18362894 | 18362894 |
Sequences
>g2513.t1 Gene=g2513 Length=1614
ATGTTAGAAAAAATGATACATGGTTTAGATAACAGTTTTATAATGAGATTTTATGAGAGA
TTGTTCATGTATTTTTCGCCAGTATCTTTAGCGATTTTTCTCATGACAATTTCATGGCTC
ATTAATTATCGTAGAAAACGTGCAAGAATGGTGAATCTTATCTCGATTATCCCTGGTCCG
CCAGCACTCCCACTCATAGGAAATGCAATCGAAGTAAATGTTGAACATGATGAAATTTTC
AGTCGCATCACGGGCATACGAAAGCTCTTCGGTAGACGCCAGGGTATTAGTAAAGGCTGG
TTTTTTGGAACTCCATATGTTTTAATCTCAAAATCTGAATGTGTAGAGCCTATATTGAGC
AATCCAAAGCACATTGATAAAAGTTTCGAATATGATTTTCTGCATCCATGGCTCGGTACT
GGCCTTTTAACAAGTCACGGACAGAAATGGTTTACTCGTAGAAAGATTTTAACACCGGCA
TTTCATTTTAAAATTTTGGAAGATTTTATGGACATTTTCAATGAGCAGAGTGCTATTTTG
GTGAAAAAACTAGAAAAGGAAATTGATTGTGATTCTTTCAATATTTTCTCTTATGTGACT
TTGTGCACATTGGATATTGTTTGTGAGACTGCTATGGGTCAACATGTTAATGCACAAGGA
AATAAAGATTCAGATTATGTGAAGGCCGTTTACGAAATTGGCAGCATCATTCTCAATAGA
CAAGCAAAAGCATGGCTTCAACCTGATTTCATCTTTAAACTAACAAATTACTATAAAAAG
CATCAAGAGTGCATTAAAATTCTTCATGGTTTCTCTTATGGTGTAATTAGAGAAAGAAGA
AAGCAGATTAATCAAAAATCAAGTGATAACAATAATAACACAAAAAATGATGAGAATGGA
AATCAAATAGAAGACGATTTTGGTAAAAAGAAAAGACTTGCCTTTCTCGACCTGCTTATT
ACAGCATCAGAAAATGGTTCACTCCTAAGCGATGAAGATATTCGTGAAGAAGTTGACACT
TTTATGTTTGAAGGACAAGATACGACCGCAAGTGCCGTATCTTGGTGTTTATTTCTGCTC
GGAGGAAATCCAAAAATTCAAGATCGAGTTTTTCAAGAAATTGACACAATTATGGGTGGT
GATAGAGAACGTGCACCAACAATGAAAGAATTAAATGAAATGAAATATTTGGAATGTTGC
ATTAAAGAAGCTTTGAGACTTTACCCAAGTGTTCCAATAATAGCAAGACATTTAAAAGAG
GATGTAAAAATAGATGAATATATTGTTCCTGCTGGCACAAGTGCAATGATTATTACATAC
ATGCTACATAGGGATCCTAACATATATCAACATCCCGAACGTTACAATCCGGATCGTTTT
CTACCGGAAAATTGCATTGGAAGACATCCATATTCATACATTCCTTTTTCAGCTGGCATG
AGAAATTGTATTGGACAAAAGTTTGCTATACTTGAAGAAAAAGCAATAATATCGAGTGTT
TTAAGAAAGTATCAAATAGAAGCTGTTGACAGAAGAGAAAATCTAACTCTATTAGGAGAA
TTAATTCTTCGTCCAAAAGATGGTCTGGCTATAAAGATAACAAAAAGGATTTAG
>g2513.t1 Gene=g2513 Length=537
MLEKMIHGLDNSFIMRFYERLFMYFSPVSLAIFLMTISWLINYRRKRARMVNLISIIPGP
PALPLIGNAIEVNVEHDEIFSRITGIRKLFGRRQGISKGWFFGTPYVLISKSECVEPILS
NPKHIDKSFEYDFLHPWLGTGLLTSHGQKWFTRRKILTPAFHFKILEDFMDIFNEQSAIL
VKKLEKEIDCDSFNIFSYVTLCTLDIVCETAMGQHVNAQGNKDSDYVKAVYEIGSIILNR
QAKAWLQPDFIFKLTNYYKKHQECIKILHGFSYGVIRERRKQINQKSSDNNNNTKNDENG
NQIEDDFGKKKRLAFLDLLITASENGSLLSDEDIREEVDTFMFEGQDTTASAVSWCLFLL
GGNPKIQDRVFQEIDTIMGGDRERAPTMKELNEMKYLECCIKEALRLYPSVPIIARHLKE
DVKIDEYIVPAGTSAMIITYMLHRDPNIYQHPERYNPDRFLPENCIGRHPYSYIPFSAGM
RNCIGQKFAILEEKAIISSVLRKYQIEAVDRRENLTLLGELILRPKDGLAIKITKRI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g2513.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 53 | 536 | 1.7E-122 |
| 21 | g2513.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 283 | 302 | - |
| 2 | g2513.t1 | PANTHER | PTHR24291:SF157 | CYTOCHROME P450 4V2 | 26 | 536 | 2.2E-182 |
| 3 | g2513.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 26 | 536 | 2.2E-182 |
| 5 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 333 | 350 | 8.4E-23 |
| 12 | g2513.t1 | PRINTS | PR00385 | P450 superfamily signature | 344 | 361 | 3.5E-12 |
| 4 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 353 | 379 | 8.4E-23 |
| 8 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 398 | 416 | 8.4E-23 |
| 10 | g2513.t1 | PRINTS | PR00385 | P450 superfamily signature | 399 | 410 | 3.5E-12 |
| 7 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 438 | 462 | 8.4E-23 |
| 6 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 473 | 483 | 8.4E-23 |
| 11 | g2513.t1 | PRINTS | PR00385 | P450 superfamily signature | 474 | 483 | 3.5E-12 |
| 9 | g2513.t1 | PRINTS | PR00463 | E-class P450 group I signature | 483 | 506 | 8.4E-23 |
| 13 | g2513.t1 | PRINTS | PR00385 | P450 superfamily signature | 483 | 494 | 3.5E-12 |
| 1 | g2513.t1 | Pfam | PF00067 | Cytochrome P450 | 58 | 529 | 3.0E-115 |
| 16 | g2513.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 20 | - |
| 18 | g2513.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 41 | - |
| 17 | g2513.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 42 | 537 | - |
| 20 | g2513.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 476 | 485 | - |
| 14 | g2513.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 57 | 536 | 6.16E-122 |
| 19 | g2513.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 43 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K15001 (cytochrome P450 family 4 [EC:1.14.-.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
