Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Trypsin-4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2599 g2599.t2 isoform g2599.t2 18994256 18996558
chr_3 g2599 g2599.t2 exon g2599.t2.exon1 18994256 18994819
chr_3 g2599 g2599.t2 cds g2599.t2.CDS1 18994306 18994819
chr_3 g2599 g2599.t2 exon g2599.t2.exon2 18996398 18996558
chr_3 g2599 g2599.t2 cds g2599.t2.CDS2 18996398 18996558
chr_3 g2599 g2599.t2 TTS g2599.t2 18997172 18997172
chr_3 g2599 g2599.t2 TSS g2599.t2 NA NA

Sequences

>g2599.t2 Gene=g2599 Length=725
AAGAAAATGAATTTGGTGAAGGATTAGAAAGAATAGTGGGAGGACAAATTATGCAAATTG
AAAGTGTTCCATATATGGTATCAATATTTTACAAAAACAATCATCTTTGCGGCGGGTCTA
TTATCTCTAGACAATGGATTATATCTGCAGCACACTGTGTTGTAGATACAGACGCGAAAA
ATTATTGGGTGAGAAGTGGATCTTCAAGGAAATCACGAGGTGGAACTGTTACAAACGCAG
AGAAAATAATTCCTCATCCCGATTATGATACAAAAACGCTTAACTATGATTTCATGCTTC
TTAAAATTTCACATCCACTCATCTATTCAGAACGACAGCAGCCAATCAAAATTGCAGACG
ATTCATCACGAGCTTATTTTGCAGGAGAAGAAGTTTTGACTAGCGGTTTTGGACTGACTC
AAAATGATATGGAATCTAATGAATTTTTGCGTGGAGTTATTGTCAAATTTTCAAGTAAAA
ATGAATGTTTAAAAGCATATCCAGAAATAATCACCGAAAATATGGTTTGTGCTGGCTCCC
AAAATAATCAAGATTCTTGTCAAGGTGATAGTGGTGGGCCTTTGGAATCACTCAGAACAG
GTGAATTAGTCGGAATCGTCAGCTTCGGAATGTCATGTGCAGACCCTTCATATCCTGGTG
TATATTCAAAAGTATCAAAAGTGAAAGACTGGATTGAACAAACGAAATCTGAAGTTGATA
ATTGA

>g2599.t2 Gene=g2599 Length=224
MQIESVPYMVSIFYKNNHLCGGSIISRQWIISAAHCVVDTDAKNYWVRSGSSRKSRGGTV
TNAEKIIPHPDYDTKTLNYDFMLLKISHPLIYSERQQPIKIADDSSRAYFAGEEVLTSGF
GLTQNDMESNEFLRGVIVKFSSKNECLKAYPEIITENMVCAGSQNNQDSCQGDSGGPLES
LRTGELVGIVSFGMSCADPSYPGVYSKVSKVKDWIEQTKSEVDN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g2599.t2 CDD cd00190 Tryp_SPc 3 218 8.04751E-84
8 g2599.t2 Gene3D G3DSA:2.40.10.10 - 1 221 4.7E-64
2 g2599.t2 PANTHER PTHR24276 POLYSERASE-RELATED 2 221 2.1E-72
3 g2599.t2 PANTHER PTHR24276:SF83 GH13245P2-RELATED 2 221 2.1E-72
5 g2599.t2 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 21 36 2.5E-15
6 g2599.t2 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 76 90 2.5E-15
4 g2599.t2 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 167 179 2.5E-15
1 g2599.t2 Pfam PF00089 Trypsin 2 215 5.5E-59
10 g2599.t2 ProSitePatterns PS00134 Serine proteases, trypsin family, histidine active site. 31 36 -
12 g2599.t2 ProSiteProfiles PS50240 Serine proteases, trypsin domain profile. 1 220 29.896
11 g2599.t2 SMART SM00020 trypsin_2 2 215 7.0E-67
7 g2599.t2 SUPERFAMILY SSF50494 Trypsin-like serine proteases 3 218 5.71E-71

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004252 serine-type endopeptidase activity MF
GO:0006508 proteolysis BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed