General information for g2803.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as RNA helicase aquarius.
Parent gene
- The parent gene g2803 is located on chr_3: 20358235..20361815 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2803 | g2803.t1 | TTS | g2803.t1 | 20358152 | 20358152 |
| chr_3 | g2803 | g2803.t1 | isoform | g2803.t1 | 20358235 | 20361815 |
| chr_3 | g2803 | g2803.t1 | exon | g2803.t1.exon1 | 20358235 | 20361432 |
| chr_3 | g2803 | g2803.t1 | cds | g2803.t1.CDS1 | 20358235 | 20361432 |
| chr_3 | g2803 | g2803.t1 | exon | g2803.t1.exon2 | 20361498 | 20361815 |
| chr_3 | g2803 | g2803.t1 | cds | g2803.t1.CDS2 | 20361498 | 20361815 |
| chr_3 | g2803 | g2803.t1 | TSS | g2803.t1 | NA | NA |
Sequences
>g2803.t1 Gene=g2803 Length=3516
ATGCTTAAATTTTATGCGCGTTTTGAAATAAACGATGAAACTGGTGACTGTTTAAGTGAA
CATGAAATGACACAACTTCATTACAAGAAAATCATATCACTTCAAAAGGCAGCGTTTGCC
AAATTTCCTGGACTAAAAGGATTTGCATTGTCCAATGTTGCGAGTGTTGACTCGCGTGAA
TCACTTACACAACATTTTGAAAACTTGGATGCAACAGCATTAAAAGAAATTGCTTCATAT
CTCAATCTCGTTCCGGACAAACTTGAACCGCCTTTCGATTGGCATCGCTTAGACGAGAAA
TTCTTGAGAAATCTTTTGATAACAAGACATGAAAGACGTGTTTCTCAACTAGAATCGCTT
AATGAGATGCCACTTTATCCAACCGAAACAATCATTTGGGATGAAAATCTTGTGCCAAAT
GAATATTTTTCTGATGGATGTCTTGCATTGCCGAAACTTAATCTTCAATTTTTAACGTTG
CACGATTATTTATATCGCAATTTTATACTCTTTCGATTGGAATCGACATATGAAATTCGT
CAAGATATTGAAGATGCTGTAATAAGGATGCTTCCTTGGCAATCTGAAGAAGGAGATGTG
GTCTTTGGTGGATGGGCAAGAATGGCATTGCCAATTCAAGAATTTGCTGTCGTAGAAGTT
GGTAAACCATTACTGGGTGAGAAACGACCGTCGCGTGTTAGAGCTGATGTTTCTGTTTCA
TTGAATGTTCGCAAAGAAATACAAGAAGAGTGGGAAAATTTGAGACGACATGATGTATGC
TTTTTAATAACAGTTCAACCAAACGTTCCGATTGGCACAAAATATAATCATCATGGAAAT
TTTATTGAACAAGTTGGATTGGTTAATGTTCGTGGATGTGAAATAGAGGGTATTTTGGAT
GAAAATGGACGAGTAATTGAAGAAGGTATTGAACCACGCTCAAAATTACCGGGTGATAAA
CGAACTTATCGAATTTGGCTTGATACAAATCAATATCGTCAAGATATGGAAGCACTTCAA
GAAGGCGAGGATGATGTCTATGAGAGTTTCAATATTTTACTACGTCGTAAACCAAAAGAA
AACAATTTCAAGGCCGTTCTTGAGACAATTCGTCATTTAATGAATACAGAATGTGTTGTT
CCACAATGGCTACATGATATTCTTTTGGGATATGGTGAACCATCCTCAGCTCATTATTCA
AAAATGTCTCTTCAAGCAAGGACTTTGGATTTCAATGATACATTTTTGAATTTTGAACAT
ATAGTTCAAAGTTTTCCTAATTATAAAGTTGTATCAAAATGCAATGATCCAAATTTGAAT
ATACCACCATTTAGATTGACATTTGAGGACGTACCTATTACTATGGATAGTGATAGTGAT
GATCAACCTGAACAAAATCTACAAAAAGTTATATTAGTGGAACCATATGTTATACCAAAA
CGTGGTCCTTATGAGTTCAATGAACCAAGAAAAAATTCAATCAAATTCACTCCAACACAA
ACAGAAGCAATTCGTTCTGGAATGCAACCAGGTTTAACACTTGTCGTTGGTCCACCTGGT
AGTGGCAAGACTGATATTGCCGTTCAAATTATTTCAAATATCTATCACAATCATCCAAAT
CAACGTACATTGATTGTTACACACTCAAATCAAGCACTTAATCAGCTTTTTGAAAAAATC
ATGCAATTAGATATTGATGAAAGACATTTATTACGCTTGGGTCATGGTGAAGAGGCACTT
GAAACTGAAAAAGATTTTAGTCGTTATGGAAGAGTGAATTATGTTCTCTCAAAGAGAATT
GAGCTTTTACAAAAAGTGAAAAAGCTGCAAGACTCGCTCGACGTTACTGGCGATGTTTCT
TATACTTGCGAAACTGCTGGACATTTTTATTTATACGAAATTGTTGCTCGATGGGAAAAG
TTTTCAAATGAATTAGTGAAAAAGGACCAACATACGCCACCAGAATATTTTACTGAAAAT
TTTCCCTTTACAAAATTCTTTAATGATGCACCACAACCATTATTCAATGGAAAATCGTTT
GATGAGAATGTTGAAATTGCAAAAAGTTGCTATCGCTATATTAATCATATTTTCACAGAA
TTAGAAGAGTTTCGTGCTTTTGAACTTCTTCGTAATGGTTTGGATCGATCTAAATATTTA
CTCGTTAAAGAAGCTAAAATTATTGCTATGACTTGTACGCATGCTGCTTTAAAACGAAAA
GAATTAGTCAATTTGAGCTTCAAATATGATAATATATTAATGGAGGAATCTGCACAAATT
TTAGAAATCGAAACGTTCATTCCACTTCTCCTGCAACAACCCCTCGATGGGTATAATCGA
TTAAAACGTTGGATTATGATTGGTGATCATTATCAATTGCCACCTGTTATTAAAAACATT
GCATTCCAAAAGTTTTCAAACATGGAGCAATCATTATTTACTCGTTTAGTGAGACTGGGA
GTCCCAGTTGTGCAACTGGATGGTCAAGGTCGTTCGAAATCATCAATTTGTGAATTGTAT
AAATGGCGATACAACATCCTTGATGATCTTCCTCATATCAAAGAATGGCCCGAATATAAT
CGAGCAAATGCAGGTTTTGTTTTTGATTATCAACTCATCAATGTCGAGAACTTTAATAAT
GTCGGCGAATCAGAACCAAATCCTTACTTTTATCAAAATTTGGGAGAAGCTGAATATGTT
GTTGCAGTATATATGTATATGCGATTGATTGGATATCCTGCTGAAAAAATATCAATTTTA
ACTACTTATAATGGTCAAAAACACTTACTACGTGACGTAATTAATCGAAGATGTGCGGAA
AATCCAGTTTTTGGTGTTCCACATAAAATCACAACTGTTGATAAATACCAAGGACAGCAA
AATGATTATGTTCTAATTTCGCTTGTGCGAACAAAAGTAGTTGGTCATTTGCGCGATGTA
AGACGTTTAGTGGTTGCTGCTAGTCGTGCAAGACTAGGTCTTTATATTTTTGGACGAGTC
TCTATTTTCAAAAATTGTTTCGAGCTTAGACCTACATTTAAGCTTTTAATGGATCACTCG
CAGAAGCTTGAATTAGTTGAAGGAGAAGTTTTTCCTACTGAACGATTATCATTGGATAAA
ATTGCAAAGAAAAAAATAAAGACAATAAATGATATGACTGAAATGGCTCAATATGTTCAT
AAAATGTATATGGTTAAAATTGAATCAATGAGAGAGGACTATGAGAAAATGAAAGAAATG
TATGAGCAAGCAGCAGCTAAAGAAATGGAAGCAAATACTGAGATGACAAATGAAAAGAGT
AACAAAAGTAATAAATCAAAGGAAAATCTTCCATTTGTTCCAATACCAATTCAAAATGAG
CTTGATGAGGAAATATTGAGTTACAATGAAGAACAATCAGAATCAGATGCAAAAAAGCAA
AAACTTGATGAAGATGATAATAATGATGTAGACTTAGCCACTGGGCAAACAACTGAAGAG
CAAGAGCAACAAGCATCAACTACAAATGACGAATGA
>g2803.t1 Gene=g2803 Length=1171
MLKFYARFEINDETGDCLSEHEMTQLHYKKIISLQKAAFAKFPGLKGFALSNVASVDSRE
SLTQHFENLDATALKEIASYLNLVPDKLEPPFDWHRLDEKFLRNLLITRHERRVSQLESL
NEMPLYPTETIIWDENLVPNEYFSDGCLALPKLNLQFLTLHDYLYRNFILFRLESTYEIR
QDIEDAVIRMLPWQSEEGDVVFGGWARMALPIQEFAVVEVGKPLLGEKRPSRVRADVSVS
LNVRKEIQEEWENLRRHDVCFLITVQPNVPIGTKYNHHGNFIEQVGLVNVRGCEIEGILD
ENGRVIEEGIEPRSKLPGDKRTYRIWLDTNQYRQDMEALQEGEDDVYESFNILLRRKPKE
NNFKAVLETIRHLMNTECVVPQWLHDILLGYGEPSSAHYSKMSLQARTLDFNDTFLNFEH
IVQSFPNYKVVSKCNDPNLNIPPFRLTFEDVPITMDSDSDDQPEQNLQKVILVEPYVIPK
RGPYEFNEPRKNSIKFTPTQTEAIRSGMQPGLTLVVGPPGSGKTDIAVQIISNIYHNHPN
QRTLIVTHSNQALNQLFEKIMQLDIDERHLLRLGHGEEALETEKDFSRYGRVNYVLSKRI
ELLQKVKKLQDSLDVTGDVSYTCETAGHFYLYEIVARWEKFSNELVKKDQHTPPEYFTEN
FPFTKFFNDAPQPLFNGKSFDENVEIAKSCYRYINHIFTELEEFRAFELLRNGLDRSKYL
LVKEAKIIAMTCTHAALKRKELVNLSFKYDNILMEESAQILEIETFIPLLLQQPLDGYNR
LKRWIMIGDHYQLPPVIKNIAFQKFSNMEQSLFTRLVRLGVPVVQLDGQGRSKSSICELY
KWRYNILDDLPHIKEWPEYNRANAGFVFDYQLINVENFNNVGESEPNPYFYQNLGEAEYV
VAVYMYMRLIGYPAEKISILTTYNGQKHLLRDVINRRCAENPVFGVPHKITTVDKYQGQQ
NDYVLISLVRTKVVGHLRDVRRLVVAASRARLGLYIFGRVSIFKNCFELRPTFKLLMDHS
QKLELVEGEVFPTERLSLDKIAKKKIKTINDMTEMAQYVHKMYMVKIESMREDYEKMKEM
YEQAAAKEMEANTEMTNEKSNKSNKSKENLPFVPIPIQNELDEEILSYNEEQSESDAKKQ
KLDEDDNNDVDLATGQTTEEQEQQASTTNDE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g2803.t1 | CDD | cd17935 | EEXXQc_AQR | 491 | 845 | 3.70299E-121 |
| 13 | g2803.t1 | CDD | cd18808 | SF1_C_Upf1 | 834 | 1016 | 3.40927E-45 |
| 11 | g2803.t1 | Coils | Coil | Coil | 1064 | 1094 | - |
| 10 | g2803.t1 | Gene3D | G3DSA:3.40.50.300 | - | 833 | 1029 | 1.7E-29 |
| 7 | g2803.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1088 | 1171 | - |
| 9 | g2803.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1131 | 1148 | - |
| 8 | g2803.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1151 | 1171 | - |
| 4 | g2803.t1 | PANTHER | PTHR10887 | DNA2/NAM7 HELICASE FAMILY | 1 | 1063 | 3.1E-284 |
| 5 | g2803.t1 | PANTHER | PTHR10887:SF5 | RNA HELICASE AQUARIUS | 1 | 1063 | 3.1E-284 |
| 2 | g2803.t1 | Pfam | PF16399 | Intron-binding protein aquarius N-terminus | 1 | 496 | 9.7E-204 |
| 1 | g2803.t1 | Pfam | PF13086 | AAA domain | 498 | 799 | 6.0E-27 |
| 3 | g2803.t1 | Pfam | PF13087 | AAA domain | 808 | 999 | 7.9E-27 |
| 6 | g2803.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 491 | 1064 | 1.64E-54 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
## Warning: Removed 1 row(s) containing missing values (geom_path).
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005681 | spliceosomal complex | CC |
| GO:0000398 | mRNA splicing, via spliceosome | BP |
| GO:0004386 | helicase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K12874 (intron-binding protein aquarius)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
