Gene loci information

Transcript annotation

  • This transcript has been annotated as Hexokinase type 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2925 g2925.t2 TSS g2925.t2 21264718 21264718
chr_3 g2925 g2925.t2 isoform g2925.t2 21264957 21273776
chr_3 g2925 g2925.t2 exon g2925.t2.exon1 21264957 21264995
chr_3 g2925 g2925.t2 cds g2925.t2.CDS1 21264957 21264995
chr_3 g2925 g2925.t2 exon g2925.t2.exon2 21271023 21271185
chr_3 g2925 g2925.t2 cds g2925.t2.CDS2 21271023 21271185
chr_3 g2925 g2925.t2 exon g2925.t2.exon3 21271251 21271435
chr_3 g2925 g2925.t2 cds g2925.t2.CDS3 21271251 21271435
chr_3 g2925 g2925.t2 exon g2925.t2.exon4 21271497 21271670
chr_3 g2925 g2925.t2 cds g2925.t2.CDS4 21271497 21271670
chr_3 g2925 g2925.t2 exon g2925.t2.exon5 21272698 21272797
chr_3 g2925 g2925.t2 cds g2925.t2.CDS5 21272698 21272797
chr_3 g2925 g2925.t2 exon g2925.t2.exon6 21272950 21273142
chr_3 g2925 g2925.t2 cds g2925.t2.CDS6 21272950 21273142
chr_3 g2925 g2925.t2 exon g2925.t2.exon7 21273203 21273542
chr_3 g2925 g2925.t2 cds g2925.t2.CDS7 21273203 21273542
chr_3 g2925 g2925.t2 exon g2925.t2.exon8 21273600 21273776
chr_3 g2925 g2925.t2 cds g2925.t2.CDS8 21273600 21273776
chr_3 g2925 g2925.t2 TTS g2925.t2 21274027 21274027

Sequences

>g2925.t2 Gene=g2925 Length=1371
ATGTCCAAAGGTGTAGTAATGACTAAACCAACAAAAGAGATCAAGGAAGCCTGTCAGGAG
TTGATATTGGATGACAAAACAATGAGAGAAATCATGAAGAGATTTCTTCATGAAATTCAA
CTTGGGTTAAAGAAAGAGACACATCCAGCAGCTGACATTAAGTGCTTCATAACTTACGTT
CAAGATTTGCCAAATGGAAAAGAAAAAGGCAGATTCCTTGCATTGGATTTAGGTGGTACG
AATTTCCGTGTCCTTCTTATTCATCTCAAAGGTGACAGTGAGTTCGAAATGCAATCAAAG
ATTTACGCCATCCCACAAAGTATTATGATTGGTTCGGGAACACAATTGTTTGACCATATT
GCTGAATGTCTTGCAAATTTCATCAAGGAACATAAACTTCAAAATGAACGGCTGCCATTA
GGTTTTACTTTCTCCTTCCCTCTTAAACAGTTAGGTTTAACAAAGGGTATTTTAATCAGA
TGGACTAAAGGTTTCAATTGTGAGGGAGTTGTTGATCGTGATGTCGTTGAACTGTTAGAA
GAGGCATTAAAAAGACGTGGAGATGTTTCTATTGAAATTTGTGCTATTCTTAATGATACA
ACTGGAACTCTTATGTCATGTGCCTGGAAAAATCACAACTGTAAAATCGGAGCTATTGTT
GGTACTGGAAGTAATGCATGTTACGTAGAGCGTGTTGAGAATGCAGAACTTTACGATGGT
CCAGCAAATGGAAAAGAATATGTGTTGATCAATACAGAGTGGGGTGCTTTTGGTGACAAT
GGTGCTCTTGATTTTATTCGTACACAGTATGATCGGGAGATGGATGAATTTAGTATCAAT
AAAGGAAAACAGACAGTTGAAAAGATGATTTCGGGAATGTATATGGGTGAATTAGTGAGA
CTTGCACTTGTTAGATTCACAAAAGAAGGACTTTTGTTTGGTGGACAAGGATCTGATTTA
CTCTTCAAACGTTACCAATTTTTCACAAAATACGTGTCTGAAATTGAGAGCGATAAGCCT
GGAACTTTCTTTAATTGTTATGATGTGCTTGAGGAAATTGGAATTTTCCATGCCACTGAT
GAGGACTGTGCAAATGTGAGATATATTTGCGAGTGCATTTCAAGTCGTGCTGCTCATTTA
TCATCAGCTGGTATTGCCACGCTTATAAACAAAATGAATGATCCAAGTGTTACTGTCGGT
GTTGATGGATCTGTCTATCGTTTCCATCCTAAATTCCATGATTTAATGATGGTAAAAATT
CGTGAACTTGTTCATGATCACATTCAATTTGACATCATGCTGTCTGAAGATGGGTCTGGA
AGAGGAGCTGCTCTTGTTGCTGCTGTTGCTTCACGTGATGCACATGAATAA

>g2925.t2 Gene=g2925 Length=456
MSKGVVMTKPTKEIKEACQELILDDKTMREIMKRFLHEIQLGLKKETHPAADIKCFITYV
QDLPNGKEKGRFLALDLGGTNFRVLLIHLKGDSEFEMQSKIYAIPQSIMIGSGTQLFDHI
AECLANFIKEHKLQNERLPLGFTFSFPLKQLGLTKGILIRWTKGFNCEGVVDRDVVELLE
EALKRRGDVSIEICAILNDTTGTLMSCAWKNHNCKIGAIVGTGSNACYVERVENAELYDG
PANGKEYVLINTEWGAFGDNGALDFIRTQYDREMDEFSINKGKQTVEKMISGMYMGELVR
LALVRFTKEGLLFGGQGSDLLFKRYQFFTKYVSEIESDKPGTFFNCYDVLEEIGIFHATD
EDCANVRYICECISSRAAHLSSAGIATLINKMNDPSVTVGVDGSVYRFHPKFHDLMMVKI
RELVHDHIQFDIMLSEDGSGRGAALVAAVASRDAHE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g2925.t2 CDD cd00012 NBD_sugar-kinase_HSP70_actin 73 255 8.5144E-9
15 g2925.t2 Gene3D G3DSA:3.40.367.20 - 19 436 1.2E-171
14 g2925.t2 Gene3D G3DSA:3.30.420.40 - 58 446 1.2E-171
3 g2925.t2 PANTHER PTHR19443:SF4 HEXOKINASE-2 11 453 3.4E-175
4 g2925.t2 PANTHER PTHR19443 HEXOKINASE 11 453 3.4E-175
8 g2925.t2 PRINTS PR00475 Hexokinase family signature 73 89 2.2E-71
7 g2925.t2 PRINTS PR00475 Hexokinase family signature 140 165 2.2E-71
5 g2925.t2 PRINTS PR00475 Hexokinase family signature 193 209 2.2E-71
6 g2925.t2 PRINTS PR00475 Hexokinase family signature 216 230 2.2E-71
11 g2925.t2 PRINTS PR00475 Hexokinase family signature 284 306 2.2E-71
10 g2925.t2 PRINTS PR00475 Hexokinase family signature 366 388 2.2E-71
9 g2925.t2 PRINTS PR00475 Hexokinase family signature 433 449 2.2E-71
2 g2925.t2 Pfam PF00349 Hexokinase 14 209 5.6E-72
1 g2925.t2 Pfam PF03727 Hexokinase 215 449 4.7E-83
17 g2925.t2 ProSitePatterns PS00378 Hexokinase domain signature. 140 165 -
18 g2925.t2 ProSiteProfiles PS51748 Hexokinase domain profile. 8 448 52.443
13 g2925.t2 SUPERFAMILY SSF53067 Actin-like ATPase domain 6 211 7.92E-78
12 g2925.t2 SUPERFAMILY SSF53067 Actin-like ATPase domain 213 452 5.83E-84

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0001678 cellular glucose homeostasis BP
GO:0005524 ATP binding MF
GO:0016773 phosphotransferase activity, alcohol group as acceptor MF
GO:0006096 glycolytic process BP
GO:0005975 carbohydrate metabolic process BP
GO:0005536 glucose binding MF
GO:0004396 hexokinase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values