Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Hexokinase type 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2925 g2925.t24 TSS g2925.t24 21267310 21267310
chr_3 g2925 g2925.t24 isoform g2925.t24 21267719 21271937
chr_3 g2925 g2925.t24 exon g2925.t24.exon1 21267719 21267782
chr_3 g2925 g2925.t24 exon g2925.t24.exon2 21271041 21271185
chr_3 g2925 g2925.t24 cds g2925.t24.CDS1 21271065 21271185
chr_3 g2925 g2925.t24 exon g2925.t24.exon3 21271251 21271435
chr_3 g2925 g2925.t24 cds g2925.t24.CDS2 21271251 21271435
chr_3 g2925 g2925.t24 exon g2925.t24.exon4 21271497 21271670
chr_3 g2925 g2925.t24 cds g2925.t24.CDS3 21271497 21271670
chr_3 g2925 g2925.t24 exon g2925.t24.exon5 21271734 21271937
chr_3 g2925 g2925.t24 cds g2925.t24.CDS4 21271734 21271772
chr_3 g2925 g2925.t24 TTS g2925.t24 NA NA

Sequences

>g2925.t24 Gene=g2925 Length=772
ATGGATTTGATAAAAAATATAAAGGACATAATTATTGGAGAACGAAGTAATTCGGATCTC
GTAAGAGTTGATATTGGATGACAAAACAATGAGAGAAATCATGAAGAGATTTCTTCATGA
AATTCAACTTGGGTTAAAGAAAGAGACACATCCAGCAGCTGACATTAAGTGCTTCATAAC
TTACGTTCAAGATTTGCCAAATGGAAAAGAAAAAGGCAGATTCCTTGCATTGGATTTAGG
TGGTACGAATTTCCGTGTCCTTCTTATTCATCTCAAAGGTGACAGTGAGTTCGAAATGCA
ATCAAAGATTTACGCCATCCCACAAAGTATTATGATTGGTTCGGGAACACAATTGTTTGA
CCATATTGCTGAATGTCTTGCAAATTTCATCAAGGAACATAAACTTCAAAATGAACGGCT
GCCATTAGGTTTTACTTTCTCCTTCCCTCTTAAACAGTTAGGTTTAACAAAGGGTATTTT
AATCAGATGGACTAAAGGTTTCAATTGTGAGGGAGTTGTTGATCGTGATGTCGTTGAACT
GTTAGAAGAGGCATTAAAAAGACGTGGATTCTATAAAGTAAAACATTGTTTTTGGCTTTT
GAAGTAATCATTTTTTTGTAGTAAGAAGGTTTGATTGAAAACCATTTATGTAAACCTTGA
TCTCTAACATATTTCATTAAAATATTTTTACTAAATTGGCATTAAGTAAAATTTACTCAA
AATCTTTCATACCTTTTTGTCCGCTTTGAAATCATTTTTGTATTAAATCACA

>g2925.t24 Gene=g2925 Length=172
MREIMKRFLHEIQLGLKKETHPAADIKCFITYVQDLPNGKEKGRFLALDLGGTNFRVLLI
HLKGDSEFEMQSKIYAIPQSIMIGSGTQLFDHIAECLANFIKEHKLQNERLPLGFTFSFP
LKQLGLTKGILIRWTKGFNCEGVVDRDVVELLEEALKRRGFYKVKHCFWLLK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g2925.t24 CDD cd00012 NBD_sugar-kinase_HSP70_actin 46 134 2.31908E-6
7 g2925.t24 Gene3D G3DSA:3.30.420.40 - 19 168 1.3E-60
2 g2925.t24 PANTHER PTHR19443:SF4 HEXOKINASE-2 3 164 7.6E-68
3 g2925.t24 PANTHER PTHR19443 HEXOKINASE 3 164 7.6E-68
4 g2925.t24 PRINTS PR00475 Hexokinase family signature 46 62 4.2E-20
5 g2925.t24 PRINTS PR00475 Hexokinase family signature 113 138 4.2E-20
1 g2925.t24 Pfam PF00349 Hexokinase 2 165 2.6E-59
9 g2925.t24 ProSitePatterns PS00378 Hexokinase domain signature. 113 138 -
10 g2925.t24 ProSiteProfiles PS51748 Hexokinase domain profile. 1 172 21.464
6 g2925.t24 SUPERFAMILY SSF53067 Actin-like ATPase domain 1 167 2.36E-65

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0001678 cellular glucose homeostasis BP
GO:0005524 ATP binding MF
GO:0016773 phosphotransferase activity, alcohol group as acceptor MF
GO:0006096 glycolytic process BP
GO:0005975 carbohydrate metabolic process BP
GO:0005536 glucose binding MF
GO:0004396 hexokinase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values