General information for g2966.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-mannosidase 2.
Parent gene
- The parent gene g2966 is located on chr_3: 21619326..21620737 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2966 | g2966.t7 | isoform | g2966.t7 | 21619326 | 21620737 |
| chr_3 | g2966 | g2966.t7 | exon | g2966.t7.exon1 | 21619326 | 21619835 |
| chr_3 | g2966 | g2966.t7 | cds | g2966.t7.CDS1 | 21619328 | 21619835 |
| chr_3 | g2966 | g2966.t7 | exon | g2966.t7.exon2 | 21619892 | 21620153 |
| chr_3 | g2966 | g2966.t7 | cds | g2966.t7.CDS2 | 21619892 | 21620153 |
| chr_3 | g2966 | g2966.t7 | exon | g2966.t7.exon3 | 21620329 | 21620737 |
| chr_3 | g2966 | g2966.t7 | cds | g2966.t7.CDS3 | 21620329 | 21620737 |
| chr_3 | g2966 | g2966.t7 | TSS | g2966.t7 | 21620868 | 21620868 |
| chr_3 | g2966 | g2966.t7 | TTS | g2966.t7 | NA | NA |
Sequences
>g2966.t7 Gene=g2966 Length=1181
ATGATCAGAATCAAAAAGAAATTCTTGCTAGCCATCTTTGTAGTTGTCTTTTTCGTATAT
CTTTACTTCTATCTCTATTCAAATGTTCCAAGTAATAGTGAAAAATTCATAGATACAGTT
CCTAAAACTATACCAAATTACAAGAAATACTCTAATGTGCATGATGTTGTTGAAAAACCA
CTTGATGATATAAATGATAATGACAACTTACTTGGGGAAGAAGATCCAGAACAGAGTAAT
TTAGGAAAAAAAGATTTAATAAATGATCAAATTCTACCGGAAAATAATAACGATGTAGAA
TCATCATGTAACTTAGATATCGATACGGTACCAAATTCTGATATTCAAATGTTAAATGTC
TACAATGAAATACCATTCGATAATTTAGACGGAGGAGTGTGGAAACAAGGATGGAATATC
GAATATGACATTCATCAGTGGAACAGAAATCACAAACTCAAAGTTTTTATTGTGCCGCAT
TCTCATAATGATCCAGGATGGATTAACACGTTTGATGAGTATTATGAGAGGCAAACTAAG
CAAATATTTTCAAATATGCTTCGACATTTGGATGAAAATGAAAATATGAAATTTATATGG
GCAGAAATTGTATATTTTTCAAGATGGTACGATAGTCTTATGACATCTGAATCAAAAGAT
AAAGTTAAACGATTAATAAAGAATAAGCAACTTGAGTTTGTCACGGGTGGTTGGGTAATG
ACAGATGAAGCAGCATCACATTGGTATTCGATTCTCCAATCTTTAACTGAGGGCCAAGTG
TGGCTTAAAAAAACATTCAATATCAGCAACATTAAAAGTTCTTGGGCAATTGATCCTTTT
GGGCATTCTTCTGTTATGCCATATATTTTTAAAGAAGCTGGTTTTGAAAACATGTTAATT
CAAAGAACACATTATTCTGTCAAAAAATATTTGGCACAAAGAAAAGAATTAGAATTCCGA
TGGAGACAACTTTGGGATACAAAAGGAACAACTGATCTCTTTACTCACATGATGCCATTC
TATTCATATGATGTTCCTCATACATGTGGTCCCGATCCGAAAATTTGTTGTCAGTTTGAT
TTCAAAAGACTTCCTGGTTATGGACTAAGCTGCCCATGGAAAGTTCCGCCGGTTCCTATC
ACTGACGATAATGTGGCAAAAAGAGCAGAAATGATAGTTGA
>g2966.t7 Gene=g2966 Length=393
MIRIKKKFLLAIFVVVFFVYLYFYLYSNVPSNSEKFIDTVPKTIPNYKKYSNVHDVVEKP
LDDINDNDNLLGEEDPEQSNLGKKDLINDQILPENNNDVESSCNLDIDTVPNSDIQMLNV
YNEIPFDNLDGGVWKQGWNIEYDIHQWNRNHKLKVFIVPHSHNDPGWINTFDEYYERQTK
QIFSNMLRHLDENENMKFIWAEIVYFSRWYDSLMTSESKDKVKRLIKNKQLEFVTGGWVM
TDEAASHWYSILQSLTEGQVWLKKTFNISNIKSSWAIDPFGHSSVMPYIFKEAGFENMLI
QRTHYSVKKYLAQRKELEFRWRQLWDTKGTTDLFTHMMPFYSYDVPHTCGPDPKICCQFD
FKRLPGYGLSCPWKVPPVPITDDNVAKRAEMIV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2966.t7 | Gene3D | G3DSA:3.20.110.10 | Glycoside hydrolase 38 | 102 | 393 | 1.2E-126 |
| 2 | g2966.t7 | PANTHER | PTHR11607:SF57 | ALPHA-MANNOSIDASE 2X | 104 | 392 | 3.2E-106 |
| 3 | g2966.t7 | PANTHER | PTHR11607 | ALPHA-MANNOSIDASE | 104 | 392 | 3.2E-106 |
| 1 | g2966.t7 | Pfam | PF01074 | Glycosyl hydrolases family 38 N-terminal domain | 154 | 392 | 9.0E-79 |
| 7 | g2966.t7 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 9 | g2966.t7 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
| 8 | g2966.t7 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 27 | 393 | - |
| 5 | g2966.t7 | SUPERFAMILY | SSF88713 | Glycoside hydrolase/deacetylase | 107 | 393 | 1.61E-100 |
| 4 | g2966.t7 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006013 | mannose metabolic process | BP |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0004559 | alpha-mannosidase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
