General information for g2974.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase PAK 3.
Parent gene
- The parent gene g2974 is located on chr_3: 21666162..21667331 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2974 | g2974.t5 | isoform | g2974.t5 | 21665360 | 21667331 |
| chr_3 | g2974 | g2974.t5 | exon | g2974.t5.exon1 | 21665360 | 21665363 |
| chr_3 | g2974 | g2974.t5 | exon | g2974.t5.exon2 | 21665606 | 21665675 |
| chr_3 | g2974 | g2974.t5 | cds | g2974.t5.CDS1 | 21665640 | 21665675 |
| chr_3 | g2974 | g2974.t5 | exon | g2974.t5.exon3 | 21665786 | 21665958 |
| chr_3 | g2974 | g2974.t5 | cds | g2974.t5.CDS2 | 21665786 | 21665958 |
| chr_3 | g2974 | g2974.t5 | exon | g2974.t5.exon4 | 21666025 | 21666098 |
| chr_3 | g2974 | g2974.t5 | cds | g2974.t5.CDS3 | 21666025 | 21666098 |
| chr_3 | g2974 | g2974.t5 | exon | g2974.t5.exon5 | 21666165 | 21667331 |
| chr_3 | g2974 | g2974.t5 | cds | g2974.t5.CDS4 | 21666165 | 21666436 |
| chr_3 | g2974 | g2974.t5 | TTS | g2974.t5 | 21667327 | 21667327 |
| chr_3 | g2974 | g2974.t5 | TSS | g2974.t5 | NA | NA |
Sequences
>g2974.t5 Gene=g2974 Length=1488
ATGGTGGCCCATTAACTGATGTTGTTACAGAAACAGTCATGAATGAGCGACAAATTTCTG
CAGTCTGTCGCGAGGTGCTTAATGCAATAAGTTTTCTTCATCAGAAAGGAATTATTCATA
GAGATATTAAGTCAGATAATGTGCTATTGGGATTAGATGGTAAAGTGAAAGTCACTGATT
TTGGATTTTGTGCAAATATTGAGGAGCATGAGAAACGTGAAACGATGGTCGGTACACCTT
ATTGGATGGCACCAGAAGTTGTAACGAGGAAACAATATGGCAAGAAAGTTGATGTCTGGT
CACTTGGAATTATGGCAATAGAAATGATTGAAGGCCAGCCACCATATTTAAATCAAGCGC
CTCTTCGAGCTCTTTATCTAATTGCAGCCAATGGCAGGCCGGAAATAAAATCTTGGGATA
AATTATCAGAGCCATTAAAAGATTTTCTCAACTGTTGCCTCGAAGTTGAAGTCGATAGAC
GAGCGTCTGCTCGTGAACTTTTAGATCATCCTTTCCTCAAAGATTGTGCAGAACTCAAGA
GTTTAACGCCACTTATTAGAGCCGCAAGACGAATTTTAAACAGGGATAAGTAAAAAGAAG
AAGAGTTCATTTAAGTTTCTGCAATTTTAATATATATTTTTCGTTGTATGTACAAGAAAA
AAAAAAGTAGTATTGAACAAAATCGAACCAATTGGATTTCAGTTAAAATCTAAATGTTAA
AACAAAAATTAATAAGAACATAATGTTGCTTAAAAATTGTTTTGATTAAGTTCTATAATT
CAAAATTGAAAGATATCTTTTAATAAAAGATACATAAAATAAGCAGCAGATAAACGCACA
AACAAATCATTTAGTAAGAAATAATGGAATATTTCATAAAAAAATACAATGGAAAATAGT
TAAATAGATTTGAATGTGAAATATTCTATAATTTCTTATATAAATAATAATAATACCCAG
AGGAAACAGTATTTGATTTAAAATTGAGATAGACAGTGATTAATTGAAGAGGAAAAAAGT
TTTTATTTGATGATGATGCGTCAGTATGTAATTAAATTTCAACAGAAGCACTCTCTTAAG
TCGAATTGTAAACAAGTGAAAAAAAGAGGAAAATTTTTATATATCTGTATTATTTTATAT
TATTATGTGTGGAAGGGAACGTGTCTAAATGTGTAATGCAATTTTAAACATTTTACATAA
ATTGTATAATCTATTTGATTTTATTACAAGTGCGCGCTTACTCTCGCTTTAGCGTTCTCA
ATTATCGGCATCATCAACTCTTTCTCACTCGCTCTCCCTCATCATAGTAGTTGCAGTAAA
AAAATGAATTATCAAATTATCACATACACACAAACATTATTTTTTTCTTACTTGTTAAAT
ATTATTATTATATATGTATATGACATGATATTTTTAGTTCTATAGAGTACTATTCTAGTT
TTCATTTTCATTTTCTTATCTAGAATAAATTTTTTGCCTCTTTATTTT
>g2974.t5 Gene=g2974 Length=184
MNERQISAVCREVLNAISFLHQKGIIHRDIKSDNVLLGLDGKVKVTDFGFCANIEEHEKR
ETMVGTPYWMAPEVVTRKQYGKKVDVWSLGIMAIEMIEGQPPYLNQAPLRALYLIAANGR
PEIKSWDKLSEPLKDFLNCCLEVEVDRRASARELLDHPFLKDCAELKSLTPLIRAARRIL
NRDK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g2974.t5 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 184 | 7.6E-69 |
| 2 | g2974.t5 | PANTHER | PTHR48015:SF8 | NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE | 1 | 183 | 1.3E-118 |
| 3 | g2974.t5 | PANTHER | PTHR48015 | SERINE/THREONINE-PROTEIN KINASE TAO | 1 | 183 | 1.3E-118 |
| 1 | g2974.t5 | Pfam | PF00069 | Protein kinase domain | 2 | 160 | 1.4E-48 |
| 6 | g2974.t5 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 25 | 37 | - |
| 8 | g2974.t5 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 160 | 36.061 |
| 5 | g2974.t5 | SMART | SM00220 | serkin_6 | 1 | 160 | 7.7E-22 |
| 4 | g2974.t5 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 2 | 165 | 1.25E-56 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
