Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative AMSH-like protease.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3021 g3021.t7 TSS g3021.t7 22170493 22170493
chr_3 g3021 g3021.t7 isoform g3021.t7 22170555 22171940
chr_3 g3021 g3021.t7 exon g3021.t7.exon1 22170555 22171379
chr_3 g3021 g3021.t7 cds g3021.t7.CDS1 22170777 22171379
chr_3 g3021 g3021.t7 exon g3021.t7.exon2 22171437 22171601
chr_3 g3021 g3021.t7 cds g3021.t7.CDS2 22171437 22171601
chr_3 g3021 g3021.t7 exon g3021.t7.exon3 22171661 22171940
chr_3 g3021 g3021.t7 TTS g3021.t7 22171996 22171996

Sequences

>g3021.t7 Gene=g3021 Length=1270
ATGGCAGATCTTATTAATTTTAATCAAATGGGGATTGTAGAACCTGAAAAGCGGTTAAAA
CAATTAAATGAGTCAGGTTCAAATATAAATGTCGATAAGAACCAGAATATCTTGAGGTAA
GTTTTTATCAATGATTCATCGTCTTCTTATTTGTTTATTATGTTGTACTGAAAATTTCAT
CATAATTGTAGATATTATAGATCTGGTAATGAAATATATAGAATGGCAAAAATATACATG
CAAGAAGGAAATCATGAAAATGCGTACATCTTATTCCTAAGATTTTTAACTTTATTTGTT
GAAAAGATAAGAGAACATCCTAATTATAAAGAAGTACCAGCTGATTTCAAAAAATCTAAC
AAAGAGAAAATGACAGAAATTATGACATTAACAGAAAAATTGAAGGCGAAACTATTGGAT
CGTTATAAAAAAGAATACGAACAATTTTTAATTGATCAAGAGAATGAGCGAAAGAAAGCG
ATTGAGGAAGCTAAACGTAAAGAAATTGAAAATGCGAAAAATAAATCCAAAATAAGTGGA
CCTTCTGCAGCATCTACAAACGTAATAATGCCTAGTGCACCTAACTTTGCTGATCTTGAT
CAAATCGTTTACCCCAATGACTTTCCAACAGATCCAAAGAAGCCTACCCAAGCTGCTGCA
AGAGATTATAAAGGTTTGCTCCCCGGTACAAAACCGCCTTCATATGATCGACTACTAAAA
CCATCGATTTCTCTTATGGAAGGTGGTTTAAGAACTTTAAAAATACCTGACGATACTATG
AAAAAGTTCCTCGATGTTGCTGCTTCAAACACTATGAAAAATATCGAAACTTGTGGCATT
CTCTGTGGAAAATTACAAAATCATCAACTTTCTATAACTCATGTAATTTTACCAAAACAA
ACTGGAACTTCAGACAGTTGTAATACAATGAACGAAGAAGAAATTTTTGATATACAAGAT
CAAATGAATTTAATTACTTTAGGGTGGTGAGATTCATACCCATCCTACTCAATCTGCATT
TCTTTCATCGGTTGATCTACATACACAATGTGGATATCAAATTATGCTACCAGAAGCTAT
TGCTATTGTTTGCTCACCTAAATATGAAGAGCTTGGCTTCTTTACTTTAACTACTGAAGG
CATTGATTTTATTTCAAAATGTACACAAACCGGATTTCATCCACATCCTAACGATCAATT
TATGGAAGCTATGCATTACAAATTAGAAAAAGATAAACACATCATAATTACTGACTTGAG
AGGTCGTTGA

>g3021.t7 Gene=g3021 Length=255
MAKIYMQEGNHENAYILFLRFLTLFVEKIREHPNYKEVPADFKKSNKEKMTEIMTLTEKL
KAKLLDRYKKEYEQFLIDQENERKKAIEEAKRKEIENAKNKSKISGPSAASTNVIMPSAP
NFADLDQIVYPNDFPTDPKKPTQAAARDYKGLLPGTKPPSYDRLLKPSISLMEGGLRTLK
IPDDTMKKFLDVAASNTMKNIETCGILCGKLQNHQLSITHVILPKQTGTSDSCNTMNEEE
IFDIQDQMNLITLGW

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g3021.t7 Coils Coil Coil 65 101 -
8 g3021.t7 Gene3D G3DSA:1.20.58.280 Hypothetical protein 1500032h18. 1 103 7.3E-24
7 g3021.t7 Gene3D G3DSA:3.40.140.10 Cytidine Deaminase 167 255 4.2E-29
3 g3021.t7 PANTHER PTHR12947 AMSH-LIKE PROTEASE 1 107 3.3E-51
4 g3021.t7 PANTHER PTHR12947 AMSH-LIKE PROTEASE 160 255 3.3E-51
1 g3021.t7 Pfam PF08969 USP8 dimerisation domain 1 70 3.7E-18
2 g3021.t7 Pfam PF01398 JAB1/Mov34/MPN/PAD-1 ubiquitin protease 177 246 7.3E-8
10 g3021.t7 ProSiteProfiles PS50249 MPN domain profile. 179 255 18.881
6 g3021.t7 SUPERFAMILY SSF140856 USP8 N-terminal domain-like 2 85 1.57E-9
5 g3021.t7 SUPERFAMILY SSF102712 JAB1/MPN domain 177 255 4.97E-6

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0008237 metallopeptidase activity MF
GO:0005515 protein binding MF
GO:0070122 isopeptidase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values