General information for g3276.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g3276 is located on chr_3: 24152566..24154984 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3276 | g3276.t1 | isoform | g3276.t1 | 24151170 | 24154984 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon1 | 24151170 | 24152019 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS1 | 24151170 | 24152019 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon2 | 24152072 | 24152314 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS2 | 24152072 | 24152314 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon3 | 24152380 | 24152519 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS3 | 24152380 | 24152519 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon4 | 24152575 | 24152701 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS4 | 24152575 | 24152701 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon5 | 24152760 | 24153119 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS5 | 24152760 | 24153119 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon6 | 24153260 | 24153544 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS6 | 24153260 | 24153544 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon7 | 24153596 | 24154153 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS7 | 24153596 | 24154153 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon8 | 24154206 | 24154432 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS8 | 24154206 | 24154432 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon9 | 24154487 | 24154616 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS9 | 24154487 | 24154616 |
| chr_3 | g3276 | g3276.t1 | exon | g3276.t1.exon10 | 24154899 | 24154984 |
| chr_3 | g3276 | g3276.t1 | cds | g3276.t1.CDS10 | 24154899 | 24154984 |
| chr_3 | g3276 | g3276.t1 | TSS | g3276.t1 | NA | NA |
| chr_3 | g3276 | g3276.t1 | TTS | g3276.t1 | NA | NA |
Sequences
>g3276.t1 Gene=g3276 Length=3006
ATGAAGCTAACGTTTGAAGAATATTGTAAATCATCAACAATTCATGGTTTCAAATATTTA
TCCAAGAAATACGATTGGATTGAAAAAATATTCTGGGTGTTTTCTTTATTGACATCTTTA
ACACTCACTTTAATTTTGATATCACAATTGATAAATAAAATTAAAACCGTTCCAATCATT
ATTTCTATTTTAGAAAAATCTATTCCAATATCTGAGATTGATTTTCCTGCAGTTACATTA
TGTCAAGGCTTAATACAAGATTTATCTAATCATAAAGAAATAACTTATTTTCAACGTCAT
TCTTATACATATAGTTACTATGACAATGATGAAGTTTTTGAAATCACAAAAGATGAAGAA
AAATTCAACAATATTTCTTATTTAACAATTTTAGATAAAATTGAAAAAGGTGAAATTAAA
CTAGAAGAACTTGGAAATAAAATATTAAAACGTTTGCAAGTGATTGATCTTTTAACACAT
CGTGGTGTTTTTGCGAGAAAAAATTTTCACATTCCAACAATTGACTTTTTGGATGTTATT
AATGAATTTAGAAAGTACAGTATATTTAAATATCCAGTTTCTTACGAATATGCATCAACA
GATAATGTAATTAGTTCTTCAGAATCAATTACAAATCTTGGAGTTTGCTTCTCTTTTAAT
AATCCTCCTTATGAATATTTACTTGATTCGACAAAAGTTTCTGATGATTTTAAGTACGAA
TTATTTATTGATCGCAAAATTGAAAAAAATCAAACGTTTATTGCACCAATTAGATCAAAA
TTGGTAAATAATATGCTTATGGTCACATTTTCAACTGATTTAACAGTAACTGAAATTAAT
AATCATATTGATGGAGGAATAATGTACATTCATGATCCTTATGAATTGCCATCTAGTTTT
TCGAAAATTATGTCAATGAACACTAACGCAAATATTGAAATTTATCTTGAACCACAATAT
ATCACAATTGATGAATCGCTTTATGATGCTAATATTTCTGAACGTAATTGTTACCATTCT
GATGAACGACAATTAAAAATCTTCAAAGTTTACACAAAAAACAACTGTTTGGTTGAATGT
TTGACAGAATTCATGATTCGAAAATGTGGATGTGTTGAATTTTTCATGATTCGAAATTAT
ACAACTCGAATTTGTTCAGCAAGTGAAAAAGAATGTTTTGATAACGCTCAACAAGAATTT
TATAATTATTTAAATAGTTGTAATTGTTTAGAATCATGCAGTTATATCAAATATGTGCTG
GATATTGTTAAAAAGCCAAATATTTATGACAACTTTTATGACAATGAATCTTTTACTCAA
ATTAATATTAAATATAAAAATTCACTTATTGATGAAATTGTAAGAAAACGACAATTTAAT
GAAGTTGATTTTCTTTCATTCGTTGGTGGTCTTTTGAATCTATTTGCTGGCTTTTCTGCT
TTGTCTTTTATTGAAATTTTGTATTGGGCATTTGCTGCAAGAATTTATGATAAACTTAAA
ATTAAAAATACTTCACTTGTTTATCCATTGGGAACTCAATTTTCGTTTAAAACTTCAAAA
AAATTTACAAATGTTCCTCAAAAATTCTTTAAGAATTCATCAGCTCATGGATTATTATAC
CTTGATGAAGAAAGCTCATTTAAAAAATTTTTTTGGATGATTTTTATTGCAATATCATTA
AGTTTGTGTTTTACAATGATTATTAAAGTTAAAGATGAGCTTTCGCATAGCTATATTGTA
GGATATGATGATGAAATGACTTTTGATGAAGAAATTAAATTCCCTGCAATTACATATCTT
CCAAATTTTTACATTGATAAAAATTTTGTTTTTAAAATTATGGGAAATATTTTTAAAAAA
AATGAACTTAATGAAATTTTGAGCAAAAATTCATCAAGAGAAAATTTTATGAAAAGAGTA
GCTGCACTTTCGCTGTGTTATCATAAATTTGTTTATAAACTTTATTCACATACTTTAAAC
GTCACAGAACAATTTTATGAAAGTTTACTAGAAAATTCACAAGATGAATGGTTTCAGCAA
CAATTAGCAACATGGAATGGAAAATTTCAAGTTAAATTTGCAAAAATGTTGACATTTTAT
GGAATGGCTTTTACTTTTAATATGATGAAATCGAAGACTCTTTTAAAGACCGAAATTGTC
AATGATGAATTTTTTTACAAATCAAACATTTCTATGGTAAAGCAAAAATCAATGCCTCAA
ATATCTAAAGAAATATTTCCATTAAAAGTAAAAGTCAAAGATCGATCACAATTTCAACTA
CTGATTCAAGAACCTAAATTTAAAAATCAAAGATTCGCTCCATGTATAAAAAGATCAGTT
ATGATTCATGATTCAGAGTTAATTCCACGCTTAATTAATTATGGTGAATTCATAAAAGTT
GATACATCAATAGATTTCGAAATAATTCCAGAAATTACAAAAACTGATAAGAATTTGAGG
AAAATTAATCCATTTGTGAGAGATTGCTATTTTAGAAATGAAAAGAGATTAAAATATTTT
AAAATTTATAACGAATATTATTGTAAATTGGAATGTTTTTTGGAAAATTCAAAGAATTCT
ATTAAATTAAATGCAACTTGTCGTTATATTGATACCATATATGGAAAAGATAAAAATCAA
AAACTTTGCACGAAAAGCACAAATTTTGAAATGTCAAACGCTCAACTTGAAATTATGGAA
AACTTTTCAGTTAAAAAGAATTGTTCATGTTTACCATTGTGCAATTCAATTAAATATCAC
ATCAAACAGTATCCAAATGCAGAAACTGGTATAAATGATTCGACAATTATTAATGTCAGA
ATGAATATAGACAACATGATTTTATTTCGTCGATATCAACAATTTTCATTTTTTGATGCT
GTTTCTTATGTTGGTGGTTTACTTGGACTTTTTGCAGGAATTTCAGTTTTATCAATTGTT
GAAATCTTTTATTTTATTACATTGCAATTGTTGAATGATATTTTGAGATTGTTAAAACGA
ACATAA
>g3276.t1 Gene=g3276 Length=1001
MKLTFEEYCKSSTIHGFKYLSKKYDWIEKIFWVFSLLTSLTLTLILISQLINKIKTVPII
ISILEKSIPISEIDFPAVTLCQGLIQDLSNHKEITYFQRHSYTYSYYDNDEVFEITKDEE
KFNNISYLTILDKIEKGEIKLEELGNKILKRLQVIDLLTHRGVFARKNFHIPTIDFLDVI
NEFRKYSIFKYPVSYEYASTDNVISSSESITNLGVCFSFNNPPYEYLLDSTKVSDDFKYE
LFIDRKIEKNQTFIAPIRSKLVNNMLMVTFSTDLTVTEINNHIDGGIMYIHDPYELPSSF
SKIMSMNTNANIEIYLEPQYITIDESLYDANISERNCYHSDERQLKIFKVYTKNNCLVEC
LTEFMIRKCGCVEFFMIRNYTTRICSASEKECFDNAQQEFYNYLNSCNCLESCSYIKYVL
DIVKKPNIYDNFYDNESFTQINIKYKNSLIDEIVRKRQFNEVDFLSFVGGLLNLFAGFSA
LSFIEILYWAFAARIYDKLKIKNTSLVYPLGTQFSFKTSKKFTNVPQKFFKNSSAHGLLY
LDEESSFKKFFWMIFIAISLSLCFTMIIKVKDELSHSYIVGYDDEMTFDEEIKFPAITYL
PNFYIDKNFVFKIMGNIFKKNELNEILSKNSSRENFMKRVAALSLCYHKFVYKLYSHTLN
VTEQFYESLLENSQDEWFQQQLATWNGKFQVKFAKMLTFYGMAFTFNMMKSKTLLKTEIV
NDEFFYKSNISMVKQKSMPQISKEIFPLKVKVKDRSQFQLLIQEPKFKNQRFAPCIKRSV
MIHDSELIPRLINYGEFIKVDTSIDFEIIPEITKTDKNLRKINPFVRDCYFRNEKRLKYF
KIYNEYYCKLECFLENSKNSIKLNATCRYIDTIYGKDKNQKLCTKSTNFEMSNAQLEIME
NFSVKKNCSCLPLCNSIKYHIKQYPNAETGINDSTIINVRMNIDNMILFRRYQQFSFFDA
VSYVGGLLGLFAGISVLSIVEIFYFITLQLLNDILRLLKRT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g3276.t1 | Gene3D | G3DSA:1.10.287.770 | - | 549 | 983 | 2.6E-17 |
| 11 | g3276.t1 | Gene3D | G3DSA:2.60.470.10 | - | 580 | 953 | 2.6E-17 |
| 12 | g3276.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 603 | 777 | 2.6E-17 |
| 13 | g3276.t1 | Gene3D | G3DSA:1.10.287.820 | - | 821 | 914 | 2.6E-17 |
| 3 | g3276.t1 | PANTHER | PTHR11690:SF222 | ACID-SENSING ION CHANNEL 5 | 4 | 492 | 5.9E-74 |
| 5 | g3276.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 4 | 492 | 5.9E-74 |
| 4 | g3276.t1 | PANTHER | PTHR11690:SF222 | ACID-SENSING ION CHANNEL 5 | 517 | 988 | 5.9E-74 |
| 6 | g3276.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 517 | 988 | 5.9E-74 |
| 2 | g3276.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 8 | 488 | 1.7E-62 |
| 1 | g3276.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 529 | 985 | 1.4E-40 |
| 18 | g3276.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 29 | - |
| 20 | g3276.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 51 | - |
| 16 | g3276.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 52 | 463 | - |
| 22 | g3276.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 464 | 491 | - |
| 19 | g3276.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 492 | 549 | - |
| 23 | g3276.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 550 | 568 | - |
| 15 | g3276.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 569 | 959 | - |
| 21 | g3276.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 960 | 986 | - |
| 17 | g3276.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 987 | 1001 | - |
| 10 | g3276.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 9 | g3276.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 492 | - |
| 8 | g3276.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 550 | 568 | - |
| 7 | g3276.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 964 | 986 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed