Gene loci information

Transcript annotation

  • This transcript has been annotated as NADP-dependent malic enzyme.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3335 g3335.t30 isoform g3335.t30 24726387 24727783
chr_3 g3335 g3335.t30 exon g3335.t30.exon1 24726387 24727558
chr_3 g3335 g3335.t30 cds g3335.t30.CDS1 24726481 24727558
chr_3 g3335 g3335.t30 exon g3335.t30.exon2 24727623 24727783
chr_3 g3335 g3335.t30 cds g3335.t30.CDS2 24727623 24727783
chr_3 g3335 g3335.t30 TTS g3335.t30 24727926 24727926
chr_3 g3335 g3335.t30 TSS g3335.t30 NA NA

Sequences

>g3335.t30 Gene=g3335 Length=1333
TGTATTAAAGAACTGGCCAGAGCCAGATGTAAGAGCTATCGTTGTTACCGATGGTGAACG
TATTTTAGGTTTAGGCGATTTAGGAGCTCAAGGAATGGGCATTCCCGTAGGAAAACTGGC
ACTCTACACTGCCCTTGCTGGTATCAAACCTCATCAGTGTTTGCCGATTGTCATCGATGT
GGGAACAAATAATAAAGATCTTCTCGAAGATCCACTTTACGTTGGTTTAAGACAACCTAG
AGTATCAGGACAAGCCTATGACGATTTTATTGATGAATTCATGGAAGCAGTTGTTAAACG
TTATGGACAAAATACTTTGATTCAATTTGAAGATTTTGGAAATCACAATGCTTTCAGGTT
TTTAGACAAATACCGTGACACTTATTGCACTTTTAATGATGATATTCAAGGCACAGCATC
AGTTGCTGTTGCAGGACTTCTTGCTTCAAAGCGTGTAACAGGAAAGAAATTGTCAGATAA
CAAAATTCTTTTCCTCGGTGCTGGTGAAGCTGCTATCGGAATTGCCGATTTGTGTGTTAA
GGCAATGATGACAGAAGGAGTTTCACAAGATGAAGCGCGCGACAAGATTTGGATGGTTGA
TATTGATGGACTTTTAGCAAAAGGACGACCAGAAGGGCAGTTAGGTGGTCACAAATGGTT
TTATGCTAAAGAGCATGAAGTTATGAAAGATTTTACTGAAATTGTAAAAGAAGTAAGGCC
ATCAATTTTAATTGGTGCAAGTGCTGTTGGTGGTGCTTTTACACCTGAAATTTTGCAAAT
GATGGCAGAATTCAATGAACGACCTATTATTTTTGCATTATCAAATCCAACAATTAAAGC
TGAATATACTACAGAAGCAGCTTATACACATACAAAAGGAAAATGCATTTTTGCTTCAGG
TTCACCATTTGGTCAAGTGAACTATAATGGACAAATTTTTAATCCTGGACAAGGAAATAA
TGCCTACATTTTTCCTGGGGTCGCTTTAGGCGTTATTGCTACAGGCACTCATCATATTCC
TGAAGACATGTTTTTAATTGCAGCTGAAACAATTGCAAAACACGTATCAGATGAAGATAT
ACAAAGAGGCTCACTATATCCACCACTTTCAGTCATTAAAGAATGCTCATTTGATATTGC
TATTGGAATTACTAAATACGCTTATGCCAAAGCAAGTTTGTATCCTGAGCCATCAGATAA
GAAAACTTGGTTGAAACAGCAACTTTATAATTTCAACTATGAAAGTTCAATGCCAATCAC
ATGGCCTTGGCCAGAAATGCCACACATTACCACCCGCCCTTTAGAACCTACTTTGCTTCA
TAAGAAAAATTAA

>g3335.t30 Gene=g3335 Length=412
MGIPVGKLALYTALAGIKPHQCLPIVIDVGTNNKDLLEDPLYVGLRQPRVSGQAYDDFID
EFMEAVVKRYGQNTLIQFEDFGNHNAFRFLDKYRDTYCTFNDDIQGTASVAVAGLLASKR
VTGKKLSDNKILFLGAGEAAIGIADLCVKAMMTEGVSQDEARDKIWMVDIDGLLAKGRPE
GQLGGHKWFYAKEHEVMKDFTEIVKEVRPSILIGASAVGGAFTPEILQMMAEFNERPIIF
ALSNPTIKAEYTTEAAYTHTKGKCIFASGSPFGQVNYNGQIFNPGQGNNAYIFPGVALGV
IATGTHHIPEDMFLIAAETIAKHVSDEDIQRGSLYPPLSVIKECSFDIAIGITKYAYAKA
SLYPEPSDKKTWLKQQLYNFNYESSMPITWPWPEMPHITTRPLEPTLLHKKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g3335.t30 CDD cd05312 NAD_bind_1_malic_enz 104 379 9.30243E-143
12 g3335.t30 Gene3D G3DSA:3.40.50.10380 - 1 101 1.5E-51
13 g3335.t30 Gene3D G3DSA:3.40.50.720 - 102 401 2.9E-116
3 g3335.t30 PANTHER PTHR23406 MALIC ENZYME-RELATED 1 386 9.4E-179
4 g3335.t30 PANTHER PTHR23406:SF80 GH17657P-RELATED 1 386 9.4E-179
14 g3335.t30 PIRSF PIRSF000106 ME 1 388 2.3E-153
6 g3335.t30 PRINTS PR00072 Malic enzyme signature 15 37 1.2E-49
8 g3335.t30 PRINTS PR00072 Malic enzyme signature 75 93 1.2E-49
9 g3335.t30 PRINTS PR00072 Malic enzyme signature 100 116 1.2E-49
5 g3335.t30 PRINTS PR00072 Malic enzyme signature 131 147 1.2E-49
7 g3335.t30 PRINTS PR00072 Malic enzyme signature 234 250 1.2E-49
1 g3335.t30 Pfam PF00390 Malic enzyme, N-terminal domain 1 94 9.7E-40
2 g3335.t30 Pfam PF03949 Malic enzyme, NAD binding domain 104 357 1.1E-96
16 g3335.t30 ProSitePatterns PS00331 Malic enzymes signature. 100 116 -
17 g3335.t30 SMART SM01274 malic_2 1 94 3.3E-22
18 g3335.t30 SMART SM00919 Malic_M_2 104 357 1.8E-110
11 g3335.t30 SUPERFAMILY SSF53223 Aminoacid dehydrogenase-like, N-terminal domain 1 103 2.3E-45
10 g3335.t30 SUPERFAMILY SSF51735 NAD(P)-binding Rossmann-fold domains 104 396 7.04E-94

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity MF
GO:0004470 malic enzyme activity MF
GO:0051287 NAD binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values