General information for g3367.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Folylpolyglutamate synthase, mitochondrial.
Parent gene
- The parent gene g3367 is located on chr_3: 24884411..24888356 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3367 | g3367.t5 | TSS | g3367.t5 | 24882348 | 24882348 |
| chr_3 | g3367 | g3367.t5 | isoform | g3367.t5 | 24882419 | 24884279 |
| chr_3 | g3367 | g3367.t5 | exon | g3367.t5.exon1 | 24882419 | 24883768 |
| chr_3 | g3367 | g3367.t5 | cds | g3367.t5.CDS1 | 24882637 | 24883768 |
| chr_3 | g3367 | g3367.t5 | exon | g3367.t5.exon2 | 24883822 | 24884279 |
| chr_3 | g3367 | g3367.t5 | cds | g3367.t5.CDS2 | 24883822 | 24884099 |
| chr_3 | g3367 | g3367.t5 | TTS | g3367.t5 | 24885195 | 24885195 |
Sequences
>g3367.t5 Gene=g3367 Length=1808
ATGATTAAATCATTAAATAACATCAAAAGGCCAATTGTAAAATTAGGTTTTCGATCTATA
TCAACAAATACAAACAAGAGAACTTTTGAGGTAAGCAGTTTTAACCAATATTATCTGGAT
AAATCAATAGTTGATAAATGAGAATAATCTTATAGGATGCTATTGTCGCGCTCAATTCTT
TACAAACAAATTCTGTCGCATTAAATCTGTCAATTCAGATGAAAAGACAACAAAAACATG
ACGAGGCAAACTTTAATCTTCGCGATACTGAAAAATATTTAGAGAGAATCGAAGGTTTCA
ATCTAAAGCAGTTAGATAAGTTATCAGTAATACATGTAAGTGGCAGTAAAGGCAAAGGAT
CAGTATGTACGTACACTGATGCAATTCTAAGAGAATATAATGTGAAAACTGGTCTCTTTA
CATCACCTCATCTTATGTCGGTGACTGAAAGAATTAAATTGATGGGTTTATCAATAAGCA
AGAGCATGTTCAATGAGTATTTTTGGCAAGTTTATGATGCTCTTCAAGCTAAAAAGAGTA
ATGATGCAGACTTGCCATCATATTTCAAGTTCCTACAAGTTATGGCTTTCTATGTGTTTG
TTAAAGAAAAAGTCGATGTTGCTATAATTGAAGTTGGTATCGGAGGACAGTTTGATTCAA
CTAATATTCTAAGAAATACAGAAATTGTAGGAATCACAGCATTGCAATTAGAGCATACTC
AATTATTGGGCAATACTCTGGAAGAGATTGCATGGCAAAAAGCAGGCATTATTAAAAAGA
ACTCTTATGTTTACGCTATGAATCAGCCTATTCAATGCATTAATGTGATTGAAAATAGAT
TTGATGAGATTGGTGGCAAAAGGTTATCTTTCATACCACCCTTCGATTATTACACATTTA
ATGAATGTCATATACCAGATTTTTCTCGGACTTCTGAAGTTAATCGGTCAAATTTTTCAC
TTGCATCACAATTAAGTGCAAGATGGTTAAGAAACAGACATAAATTATCAGAGGAGCATT
TTTGTGACAATTTGTTAAAAATCATTGATAAGAAATTTGTTGATGCATACGAGAAATGTC
ATTTTGATGGACGATTTCAAAGAATTGAAGATGATGGCATAACATATTTTCTTGATGGAG
CACATACGATTGATAGCATGAAAATCTGTTCAGAGTGGTTTGCTAAGCAAATTAATGATG
GTAATCGTGAACAATTGAATGTGCTTATTTTTAATGTAACTGGTGATCGAGATTCTGAGT
TAATATTAGAGCAATTACATTCCATTAAATTTAATTTAGTATGCTTTAGTACTAATATAC
CTGACAGTGATTCATACAATGAGCAAAGCGAAAATTTTACGGGTATCTTGAAAAACACAC
AGCTCGAAAGATGTATGAGACATAAAATTATTTGGTCAAAATATAAAGACAATACTCAAA
TTATGGTGTTTTCAACAATTCAATCCGTCATAAAATACATTCGTAACTTGAGAGAAAATA
ATACTGCACACATCAACGCTCTTTTTACTGGATCACTTCATTTAGTTGGAGGATCATTGA
AGATACTAAATGATATTGTACATGAACATAAAGAAAGACAAAGTCTATCATCAAGTAATT
CACTATAAATATCCACTTTCTTACATATCTTTGCCATTTTTGTAGCTGAAAATTTTTTGT
TTGTAACCGTCACTATAATTTTAAAAGTTTAATAAAAAAAAAGTTACGAATCAAAACAAA
GATTTTATTAAAAAAATAATTTTTCATCGCGTTTTAATAGATATTTTCTTTATTTATAAA
ATGGCAGG
>g3367.t5 Gene=g3367 Length=469
MKRQQKHDEANFNLRDTEKYLERIEGFNLKQLDKLSVIHVSGSKGKGSVCTYTDAILREY
NVKTGLFTSPHLMSVTERIKLMGLSISKSMFNEYFWQVYDALQAKKSNDADLPSYFKFLQ
VMAFYVFVKEKVDVAIIEVGIGGQFDSTNILRNTEIVGITALQLEHTQLLGNTLEEIAWQ
KAGIIKKNSYVYAMNQPIQCINVIENRFDEIGGKRLSFIPPFDYYTFNECHIPDFSRTSE
VNRSNFSLASQLSARWLRNRHKLSEEHFCDNLLKIIDKKFVDAYEKCHFDGRFQRIEDDG
ITYFLDGAHTIDSMKICSEWFAKQINDGNREQLNVLIFNVTGDRDSELILEQLHSIKFNL
VCFSTNIPDSDSYNEQSENFTGILKNTQLERCMRHKIIWSKYKDNTQIMVFSTIQSVIKY
IRNLRENNTAHINALFTGSLHLVGGSLKILNDIVHEHKERQSLSSSNSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g3367.t5 | Gene3D | G3DSA:3.40.1190.10 | - | 1 | 276 | 7.4E-76 |
| 7 | g3367.t5 | Gene3D | G3DSA:3.90.190.20 | - | 291 | 459 | 2.0E-19 |
| 1 | g3367.t5 | PANTHER | PTHR11136:SF5 | FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL | 7 | 450 | 3.0E-126 |
| 2 | g3367.t5 | PANTHER | PTHR11136 | FOLYLPOLYGLUTAMATE SYNTHASE-RELATED | 7 | 450 | 3.0E-126 |
| 5 | g3367.t5 | ProSitePatterns | PS01012 | Folylpolyglutamate synthase signature 2. | 136 | 151 | - |
| 4 | g3367.t5 | SUPERFAMILY | SSF53623 | MurD-like peptide ligases, catalytic domain | 11 | 267 | 4.01E-65 |
| 3 | g3367.t5 | SUPERFAMILY | SSF53244 | MurD-like peptide ligases, peptide-binding domain | 291 | 459 | 2.17E-16 |
| 8 | g3367.t5 | TIGRFAM | TIGR01499 | folC: bifunctional protein FolC | 17 | 449 | 1.2E-90 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004326 | tetrahydrofolylpolyglutamate synthase activity | MF |
| GO:0009058 | biosynthetic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0016874 | ligase activity | MF |
| GO:0009396 | folic acid-containing compound biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01930 (folylpolyglutamate synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
