Gene loci information

Transcript annotation

  • This transcript has been annotated as Diacylglycerol kinase theta.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3402 g3402.t1 isoform g3402.t1 25206241 25214527
chr_3 g3402 g3402.t1 exon g3402.t1.exon1 25206241 25206366
chr_3 g3402 g3402.t1 cds g3402.t1.CDS1 25206241 25206366
chr_3 g3402 g3402.t1 exon g3402.t1.exon2 25207266 25207373
chr_3 g3402 g3402.t1 cds g3402.t1.CDS2 25207266 25207373
chr_3 g3402 g3402.t1 exon g3402.t1.exon3 25207439 25207595
chr_3 g3402 g3402.t1 cds g3402.t1.CDS3 25207439 25207595
chr_3 g3402 g3402.t1 exon g3402.t1.exon4 25207657 25207945
chr_3 g3402 g3402.t1 cds g3402.t1.CDS4 25207657 25207945
chr_3 g3402 g3402.t1 exon g3402.t1.exon5 25208596 25208775
chr_3 g3402 g3402.t1 cds g3402.t1.CDS5 25208596 25208775
chr_3 g3402 g3402.t1 exon g3402.t1.exon6 25208839 25208987
chr_3 g3402 g3402.t1 cds g3402.t1.CDS6 25208839 25208987
chr_3 g3402 g3402.t1 exon g3402.t1.exon7 25209056 25209285
chr_3 g3402 g3402.t1 cds g3402.t1.CDS7 25209056 25209285
chr_3 g3402 g3402.t1 exon g3402.t1.exon8 25209361 25209847
chr_3 g3402 g3402.t1 cds g3402.t1.CDS8 25209361 25209847
chr_3 g3402 g3402.t1 exon g3402.t1.exon9 25209917 25210041
chr_3 g3402 g3402.t1 cds g3402.t1.CDS9 25209917 25210041
chr_3 g3402 g3402.t1 exon g3402.t1.exon10 25210109 25210245
chr_3 g3402 g3402.t1 cds g3402.t1.CDS10 25210109 25210245
chr_3 g3402 g3402.t1 exon g3402.t1.exon11 25210340 25210513
chr_3 g3402 g3402.t1 cds g3402.t1.CDS11 25210340 25210513
chr_3 g3402 g3402.t1 exon g3402.t1.exon12 25210757 25211407
chr_3 g3402 g3402.t1 cds g3402.t1.CDS12 25210757 25211407
chr_3 g3402 g3402.t1 exon g3402.t1.exon13 25211580 25211783
chr_3 g3402 g3402.t1 cds g3402.t1.CDS13 25211580 25211783
chr_3 g3402 g3402.t1 exon g3402.t1.exon14 25211845 25212036
chr_3 g3402 g3402.t1 cds g3402.t1.CDS14 25211845 25212036
chr_3 g3402 g3402.t1 exon g3402.t1.exon15 25212097 25212279
chr_3 g3402 g3402.t1 cds g3402.t1.CDS15 25212097 25212279
chr_3 g3402 g3402.t1 exon g3402.t1.exon16 25214416 25214527
chr_3 g3402 g3402.t1 cds g3402.t1.CDS16 25214416 25214527
chr_3 g3402 g3402.t1 TSS g3402.t1 NA NA
chr_3 g3402 g3402.t1 TTS g3402.t1 NA NA

Sequences

>g3402.t1 Gene=g3402 Length=3504
ATGGCGAATGGTGGACATTCCTTTGGAAAGAAAACATTTCATAAACCAACTTATTGCCAC
CACTGTTCGGATCTCTTGTGGGGCCTAATTGGACAAGGATACATCTGTGAAGTGTGCAAT
TTTATTGTTCATGAGAAGTGTGTCAATGGTGTTGTCACACCATGCTCGGGTATTTCACCA
TTCTTAATTAAGAATCCTGTCGCACACTGCTGGTCGGAGCCTGTTCATCACAAGAGAAAA
TTCTGTACTGTTTGTCGTAAGCGCTTGGATGAAGTTTCTGCAGTACATTGCATGATATGC
GAATATTATGCGCATATCGAATGTCAAGATTTCGCCATTGCTGATTGCAAGGAAAATGCA
ACTTACGTTCCTGGCAAAGACTTGAGTGTCGTCAAACATGTTCATCATTGGCGTGAAGGC
AATTTACCGCAATCATCAAAATGTGCATATTGCAAAAAAACGTGTTGGTCATCTGAATGT
CTTACTGGCTATCGTTGTGAATGGTGTGGATTAACAACTCATGGCGGATGTCGACATTTC
GTCCAACAAGAATGCACTTTTGGCTCTCTTCAGCCGATTTATCTGCCTCCACATGCAGTC
TCAATTCCGCGAACAGAAGTTCCAATGGAAGCAATAATTGGTGTGCAAATTAAGAACAAG
GGAATTCCGATGCAACGCGAATATTCATGCCCTGAACTCCGTACGATAAGTTTATGTGAT
AGTGATAGCGATCTATTTATGCAAACATATAATGCACTACATCGAAAGTCGAGTGATTCA
TCACACTCGGCTGCAGTTTCTTACTACAGTGGCACTGAGAGCCAACAAAGTGTGAGTGAA
CGATCGTTTTACGATAATGTCTTTGAACATAATCGCCAGCCAAATCATCATCATCACCAT
CATAGTAATAGTAGTAGTTCACATGTTGTATCGCAAAATCATCCTAATAGCAATCGACAT
TTTACAAAGGCAAGAAATTTTCTTATGCCGCCAAGAAATAATGCAAATGTTAATCGTAGT
GTATCCTTTCAAACAACCTTACTTTCACCTCGTTCAAATGTCATATTAAGGAGAAGAGGT
CTTAATGATAGTTTTCACACTGACACTGAAAGCGCTTCAAACCGTTCATCAGTTTCACCG
TCCGATTCAAGTCCTTCTAAAACTAACGTTGACTGTAGTGTTCCGTGTAAATCTGATGTA
CAAAATCATAGATTAATGGACGATAATATTAGCAATAATAGACGTGCAACATACAGTGGA
CCTTTTTATATTAAATTATTACGACGAATGCAAAAGATTTCTACTTTTTGGCGTAAGCGT
GAAAGTAATAAGGCGAATAGAGCACGCAGCATATCAGACGATTTAAGTAGTGGTGATGCT
GCACGCTTTCGAGATGATGAGTATGGAAAAACTGAAACAGCAAGTGGAATGAGTAGTTCC
AGTGCACGCAATGAGTCGGCGCATAAGGGTGAAAGAGATAAGGAGAAAAAAGAAAAAGAC
AAAGAGGAGTTTGAAGAAACAATTAAAGTTTTCGATGGCAACTGTTCTCTCCGGCGACGA
ATATTTCGCGTAATCACCGTTCCACGAACGTGCAATTTGGAGCAATTATTGACAACTGCT
TTACGAGCTTTTCATATTGCAAGAGAGCCTAATCAATTTTATTTGACGGACTTATATGCA
ACTAATGGCGATGATGATGTTCATATTCAAGATCCAACACCAATCTTGAATTTACATAAG
ATTGAAGGAAAACGTCCAGCTATATTTTTAAGATTTCGTGAAAAGGAAATTGATAAAGGA
TTTGTGCGTGTATATCCGGGCAAATTGCAAGTAGAAGAAGCATATGTGACTGTTCAAGTG
GATAACGACACAACGGTTAAAGACCTAATACGCGATTCACTCAATAATTTCGGCTTGCAG
GACCATCAAGTTGATGACTATCGATGTTTAGAAGTGCTCCTTGATCGAGGCGTAACTGAA
CGTGTATTATCGTGGAATGAACGACCATGGGAAATAATGAAACAATTAGCGAAAGATTCA
ATTAGACAAATGGAATTAATGCGCTTTTACTTGCAATTAAAACAGGATCCACATGGACCA
AATTTAGCATTATTCGTTGGAAATTTACCACCAGGTCTTTCTCAACGTAATTATGAGCAA
ATTTTAACAGAATTTTTGGGTGTGGAGAATAAATTTTCGAGCATTGGTCCAATTTACTAT
GAATATGGCTCTCTTGTCATCACTTATGAGAATGCATCAAAGGCTGTACGAGCTCTTTAT
GCCCTTAGAGAATCAAAGTATGAGGAAAAACATTTACTTGTGCTTCTGCTGCCGAATATC
GAGCCATCGATGGTACCAACAGGTGTCAAACCATTACTAGTGTTTGTAAATGTGAAATCG
GGTGGATGTCAAGGATTAGAACTAATTAATAGCTTCAGAAAATTATTGAATCCTTATCAA
GTATTTGATTTGGATAATGGCGGTCCATTACCAGGGTTGTATGTTTTTAGACACATTCAA
GATTATAAAATTCTCGTATGTGGTGGTGATGGTACAATTGGTTGGGTTCTACAATGTTTG
GATAATGTAGGTCAAGATTCAGAATGTTCTAGTCCACCATGTGCTATTGTACCATTAGGA
ACTGGAAATGATTTGGCACGTGTTCTTAGATGGGGTGCTGGTTACACCGGAGGCGAAGAT
CCATTAAATTTATTACGTGATGTCATTGATGCAGAAGAAATTCGTCTCGACCGGTGGACA
GTAGTTTTTCATCCAGAAGATAAACCTGAAGATGTAGCGCCTAAAGCGCCAACAAATTCA
ACTGTTGTTGGAGGTCAAAGTGAAGATAATTCACAAATTTTCGTCATGAACAACTATTTT
GGCATTGGCATCGATGCAGATCTCTGCTTGGACTTCCATAATGCTCGCGAAGAAAATCCA
AATAAATTCAACTCACGCTTGCACAATAAAGGTGTTTATGTTAAAATGGGACTAAGAAAA
ATGGTCGGTAGAAAGATGTGTAAGGATCTGCACAAAGAAATACGCTTGGAGGTTGATGGT
AAACATGTTGAACTGCCACCTGTTGAAGGAATCATAATATTGAATATTTTGAGTTGGGGA
TCAGGAGCAAATCCATGGGGGCCAGAAAAAGAAGATGCCTTCTCGAAACCAAATCACTGG
GATGGAATGCTGGAAGTGGTCGGTGTAACAGGTGTTGTGCATTTAGGACAAATTCAATCG
GGTCTAAGATCAGCAATGAGAATTGCTCAGGGTGGTCATATAAAAATTCATCTTCATTCG
GACATACCGGTTCAAGTAGATGGTGAACCATGGGTACAAAGTCCAGGCGATGTTGTCGTA
TTAAAGTCAGCACTGAAGGCAACGATGTTGAAGAAAATAAAAAGCAAAAGGAGATTAACG
GAGCCACATATTTCGCCTGCTGTTGCTGGCGCTCCCGATCGAGAAAATGGAGATCGCGAC
AAGGAACCGTTGACTGGCACATAG

>g3402.t1 Gene=g3402 Length=1167
MANGGHSFGKKTFHKPTYCHHCSDLLWGLIGQGYICEVCNFIVHEKCVNGVVTPCSGISP
FLIKNPVAHCWSEPVHHKRKFCTVCRKRLDEVSAVHCMICEYYAHIECQDFAIADCKENA
TYVPGKDLSVVKHVHHWREGNLPQSSKCAYCKKTCWSSECLTGYRCEWCGLTTHGGCRHF
VQQECTFGSLQPIYLPPHAVSIPRTEVPMEAIIGVQIKNKGIPMQREYSCPELRTISLCD
SDSDLFMQTYNALHRKSSDSSHSAAVSYYSGTESQQSVSERSFYDNVFEHNRQPNHHHHH
HSNSSSSHVVSQNHPNSNRHFTKARNFLMPPRNNANVNRSVSFQTTLLSPRSNVILRRRG
LNDSFHTDTESASNRSSVSPSDSSPSKTNVDCSVPCKSDVQNHRLMDDNISNNRRATYSG
PFYIKLLRRMQKISTFWRKRESNKANRARSISDDLSSGDAARFRDDEYGKTETASGMSSS
SARNESAHKGERDKEKKEKDKEEFEETIKVFDGNCSLRRRIFRVITVPRTCNLEQLLTTA
LRAFHIAREPNQFYLTDLYATNGDDDVHIQDPTPILNLHKIEGKRPAIFLRFREKEIDKG
FVRVYPGKLQVEEAYVTVQVDNDTTVKDLIRDSLNNFGLQDHQVDDYRCLEVLLDRGVTE
RVLSWNERPWEIMKQLAKDSIRQMELMRFYLQLKQDPHGPNLALFVGNLPPGLSQRNYEQ
ILTEFLGVENKFSSIGPIYYEYGSLVITYENASKAVRALYALRESKYEEKHLLVLLLPNI
EPSMVPTGVKPLLVFVNVKSGGCQGLELINSFRKLLNPYQVFDLDNGGPLPGLYVFRHIQ
DYKILVCGGDGTIGWVLQCLDNVGQDSECSSPPCAIVPLGTGNDLARVLRWGAGYTGGED
PLNLLRDVIDAEEIRLDRWTVVFHPEDKPEDVAPKAPTNSTVVGGQSEDNSQIFVMNNYF
GIGIDADLCLDFHNAREENPNKFNSRLHNKGVYVKMGLRKMVGRKMCKDLHKEIRLEVDG
KHVELPPVEGIIILNILSWGSGANPWGPEKEDAFSKPNHWDGMLEVVGVTGVVHLGQIQS
GLRSAMRIAQGGHIKIHLHSDIPVQVDGEPWVQSPGDVVVLKSALKATMLKKIKSKRRLT
EPHISPAVAGAPDRENGDRDKEPLTGT

Protein features from InterProScan

Transcript Database ID Name Start End E.value
26 g3402.t1 CDD cd00029 C1 6 55 5.05681E-16
27 g3402.t1 CDD cd00029 C1 135 185 6.41983E-13
29 g3402.t1 CDD cd17111 RA1_DAGK-theta 507 595 9.61885E-35
28 g3402.t1 CDD cd01783 RA2_DAGK-theta 600 694 3.80786E-44
25 g3402.t1 Coils Coil Coil 487 507 -
24 g3402.t1 Gene3D G3DSA:3.30.60.20 - 4 60 1.2E-21
23 g3402.t1 Gene3D G3DSA:3.30.60.20 - 133 186 8.6E-18
20 g3402.t1 Gene3D G3DSA:3.10.20.90 - 593 699 4.0E-16
22 g3402.t1 Gene3D G3DSA:3.40.50.10330 - 788 900 3.9E-9
21 g3402.t1 Gene3D G3DSA:2.60.200.40 - 952 1122 2.3E-17
41 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 294 316 -
44 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 366 392 -
43 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 462 503 -
40 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 485 503 -
42 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 1136 1167 -
39 g3402.t1 MobiDBLite mobidb-lite consensus disorder prediction 1153 1167 -
7 g3402.t1 PANTHER PTHR11255:SF34 DIACYLGLYCEROL KINASE THETA 73 1138 0.0
8 g3402.t1 PANTHER PTHR11255 DIACYLGLYCEROL KINASE 73 1138 0.0
9 g3402.t1 PRINTS PR00008 Diacylglycerol/phorbol-ester binding signature 3 17 2.7E-7
10 g3402.t1 PRINTS PR00008 Diacylglycerol/phorbol-ester binding signature 19 28 2.7E-7
12 g3402.t1 PRINTS PR00008 Diacylglycerol/phorbol-ester binding signature 32 43 2.7E-7
11 g3402.t1 PRINTS PR00008 Diacylglycerol/phorbol-ester binding signature 44 56 2.7E-7
5 g3402.t1 Pfam PF00130 Phorbol esters/diacylglycerol binding domain (C1 domain) 6 55 4.4E-12
6 g3402.t1 Pfam PF00130 Phorbol esters/diacylglycerol binding domain (C1 domain) 135 186 1.1E-10
3 g3402.t1 Pfam PF00788 Ras association (RalGDS/AF-6) domain 506 593 1.2E-6
4 g3402.t1 Pfam PF00788 Ras association (RalGDS/AF-6) domain 598 696 2.6E-18
2 g3402.t1 Pfam PF00781 Diacylglycerol kinase catalytic domain 791 893 1.1E-23
1 g3402.t1 Pfam PF00609 Diacylglycerol kinase accessory domain 955 1110 2.2E-57
37 g3402.t1 ProSitePatterns PS00479 Zinc finger phorbol-ester/DAG-type signature. 6 55 -
38 g3402.t1 ProSitePatterns PS00479 Zinc finger phorbol-ester/DAG-type signature. 135 185 -
47 g3402.t1 ProSiteProfiles PS50081 Zinc finger phorbol-ester/DAG-type profile. 5 55 15.169
49 g3402.t1 ProSiteProfiles PS50081 Zinc finger phorbol-ester/DAG-type profile. 68 116 9.649
48 g3402.t1 ProSiteProfiles PS50081 Zinc finger phorbol-ester/DAG-type profile. 134 185 12.055
46 g3402.t1 ProSiteProfiles PS50200 Ras-associating (RA) domain profile. 598 696 20.436
45 g3402.t1 ProSiteProfiles PS50102 Eukaryotic RNA Recognition Motif (RRM) profile. 702 779 8.552
50 g3402.t1 ProSiteProfiles PS50146 DAG-kinase catalytic (DAGKc) domain profile. 787 925 40.464
32 g3402.t1 SMART SM00109 c1_12 6 55 1.8E-14
31 g3402.t1 SMART SM00109 c1_12 69 116 0.0031
33 g3402.t1 SMART SM00109 c1_12 135 185 3.6E-11
34 g3402.t1 SMART SM00314 RA_5 504 595 3.2
35 g3402.t1 SMART SM00314 RA_5 598 696 2.4E-14
36 g3402.t1 SMART SM00046 dagk_c4a_7 791 919 1.2E-57
30 g3402.t1 SMART SM00045 dagk_c4b_2 955 1110 7.9E-83
14 g3402.t1 SUPERFAMILY SSF57889 Cysteine-rich domain 4 56 1.64E-15
15 g3402.t1 SUPERFAMILY SSF57889 Cysteine-rich domain 66 119 1.24E-7
13 g3402.t1 SUPERFAMILY SSF57889 Cysteine-rich domain 127 190 1.09E-13
17 g3402.t1 SUPERFAMILY SSF54236 Ubiquitin-like 507 565 1.55E-6
18 g3402.t1 SUPERFAMILY SSF54236 Ubiquitin-like 599 696 9.04E-20
16 g3402.t1 SUPERFAMILY SSF54928 RNA-binding domain, RBD 698 776 4.49E-5
19 g3402.t1 SUPERFAMILY SSF111331 NAD kinase/diacylglycerol kinase-like 790 1128 5.67E-58

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004143 diacylglycerol kinase activity MF
GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway BP
GO:0003951 NAD+ kinase activity MF
GO:0007165 signal transduction BP
GO:0016301 kinase activity MF
GO:0035556 intracellular signal transduction BP
GO:0003676 nucleic acid binding MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values