General information for g3402.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol kinase theta.
Parent gene
- The parent gene g3402 is located on chr_3: 25210340..25214899 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3402 | g3402.t3 | isoform | g3402.t3 | 25210340 | 25214899 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon1 | 25210340 | 25210513 |
| chr_3 | g3402 | g3402.t3 | cds | g3402.t3.CDS1 | 25210341 | 25210513 |
| chr_3 | g3402 | g3402.t3 | TTS | g3402.t3 | 25210372 | 25210372 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon2 | 25211580 | 25211783 |
| chr_3 | g3402 | g3402.t3 | cds | g3402.t3.CDS2 | 25211580 | 25211783 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon3 | 25211845 | 25212036 |
| chr_3 | g3402 | g3402.t3 | cds | g3402.t3.CDS3 | 25211845 | 25212036 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon4 | 25212097 | 25212279 |
| chr_3 | g3402 | g3402.t3 | cds | g3402.t3.CDS4 | 25212097 | 25212279 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon5 | 25214416 | 25214689 |
| chr_3 | g3402 | g3402.t3 | cds | g3402.t3.CDS5 | 25214416 | 25214527 |
| chr_3 | g3402 | g3402.t3 | exon | g3402.t3.exon6 | 25214760 | 25214899 |
| chr_3 | g3402 | g3402.t3 | TSS | g3402.t3 | NA | NA |
Sequences
>g3402.t3 Gene=g3402 Length=1167
AAAAGAAAAAAAGTAAAAGGCAGCAAAGTAAAAGCAAGAAAATAAGAGGAAGTCAAATTT
TTCGTAAAGCTCTCAACGCGACCAACTGACTCATTACAAACATACACGCAAGCATCATCA
TCTATCTCTAGATATTCCCTTAACAATTCTACATAACACACGAAATTCAGCAAACAATTC
TCATTCAAAATTGCAACATTGACATAAAACAGCAGCAGCAGAAACAATAATCGAGTGTCA
AAAGTGACAAAATAAAAATTTCAAAATTTCAAAGCACGGAGCATCTCAACAACTTTCACA
AAATGGCGAATGGTGGACATTCCTTTGGAAAGAAAACATTTCATAAACCAACTTATTGCC
ACCACTGTTCGGATCTCTTGTGGGGCCTAATTGGACAAGGATACATCTGTGAAGTGTGCA
ATTTTATTGTTCATGAGAAGTGTGTCAATGGTGTTGTCACACCATGCTCGGGTATTTCAC
CATTCTTAATTAAGAATCCTGTCGCACACTGCTGGTCGGAGCCTGTTCATCACAAGAGAA
AATTCTGTACTGTTTGTCGTAAGCGCTTGGATGAAGTTTCTGCAGTACATTGCATGATAT
GCGAATATTATGCGCATATCGAATGTCAAGATTTCGCCATTGCTGATTGCAAGGAAAATG
CAACTTACGTTCCTGGCAAAGACTTGAGTGTCGTCAAACATGTTCATCATTGGCGTGAAG
GCAATTTACCGCAATCATCAAAATGTGCATATTGCAAAAAAACGTGTTGGTCATCTGAAT
GTCTTACTGGCTATCGTTGTGAATGGTGTGGATTAACAACTCATGGCGGATGTCGACATT
TCGTCCAACAAGAATGCACTTTTGGCTCTCTTCAGCCGATTTATCTGCCTCCACATGCAG
TCTCAATTCCGCGAACAGAAGTTCCAATGGAAGCAATAATTGGTGTGCAAATTAAGAACA
AGGGAATTCCGATGCAACGCGAATATTCATGCCCACGCAGCATATCAGACGATTTAAGTA
GTGGTGATGCTGCACGCTTTCGAGATGATGAGTATGGAAAAACTGAAACAGCAAGTGGAA
TGAGTAGTTCCAGTGCACGCAATGAGTCGGCGCATAAGGGTGAAAGAGATAAGGAGAAAA
AAGAAAAAGACAAAGAGGAGTTTGAAG
>g3402.t3 Gene=g3402 Length=288
MANGGHSFGKKTFHKPTYCHHCSDLLWGLIGQGYICEVCNFIVHEKCVNGVVTPCSGISP
FLIKNPVAHCWSEPVHHKRKFCTVCRKRLDEVSAVHCMICEYYAHIECQDFAIADCKENA
TYVPGKDLSVVKHVHHWREGNLPQSSKCAYCKKTCWSSECLTGYRCEWCGLTTHGGCRHF
VQQECTFGSLQPIYLPPHAVSIPRTEVPMEAIIGVQIKNKGIPMQREYSCPRSISDDLSS
GDAARFRDDEYGKTETASGMSSSSARNESAHKGERDKEKKEKDKEEFE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g3402.t3 | CDD | cd00029 | C1 | 6 | 55 | 8.45118E-12 |
| 15 | g3402.t3 | CDD | cd00029 | C1 | 135 | 185 | 5.79723E-9 |
| 13 | g3402.t3 | Gene3D | G3DSA:3.30.60.20 | - | 4 | 60 | 1.7E-22 |
| 12 | g3402.t3 | Gene3D | G3DSA:3.30.60.20 | - | 133 | 186 | 1.3E-18 |
| 22 | g3402.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 233 | 288 | - |
| 21 | g3402.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 268 | 288 | - |
| 3 | g3402.t3 | PANTHER | PTHR11255 | DIACYLGLYCEROL KINASE | 68 | 205 | 3.5E-26 |
| 4 | g3402.t3 | PANTHER | PTHR11255:SF33 | DIACYLGLYCEROL KINASE KAPPA | 68 | 205 | 3.5E-26 |
| 5 | g3402.t3 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 3 | 17 | 1.9E-9 |
| 6 | g3402.t3 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 19 | 28 | 1.9E-9 |
| 8 | g3402.t3 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 32 | 43 | 1.9E-9 |
| 7 | g3402.t3 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 44 | 56 | 1.9E-9 |
| 1 | g3402.t3 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 6 | 55 | 6.8E-13 |
| 2 | g3402.t3 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 135 | 186 | 1.6E-11 |
| 19 | g3402.t3 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 6 | 55 | - |
| 20 | g3402.t3 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 135 | 185 | - |
| 23 | g3402.t3 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 5 | 55 | 15.169 |
| 25 | g3402.t3 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 68 | 116 | 9.649 |
| 24 | g3402.t3 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 134 | 185 | 12.055 |
| 17 | g3402.t3 | SMART | SM00109 | c1_12 | 6 | 55 | 1.8E-14 |
| 16 | g3402.t3 | SMART | SM00109 | c1_12 | 69 | 116 | 0.0031 |
| 18 | g3402.t3 | SMART | SM00109 | c1_12 | 135 | 185 | 3.6E-11 |
| 10 | g3402.t3 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 4 | 56 | 2.54E-16 |
| 11 | g3402.t3 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 66 | 119 | 1.74E-8 |
| 9 | g3402.t3 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 130 | 190 | 4.78E-15 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004143 | diacylglycerol kinase activity | MF |
| GO:0007165 | signal transduction | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed