General information for g345.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Apyrase.
Parent gene
- The parent gene g345 is located on chr_3: 2807485..2809329 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g345 | g345.t1 | TTS | g345.t1 | 2806613 | 2806613 |
| chr_3 | g345 | g345.t1 | isoform | g345.t1 | 2806952 | 2809329 |
| chr_3 | g345 | g345.t1 | exon | g345.t1.exon1 | 2806952 | 2807476 |
| chr_3 | g345 | g345.t1 | cds | g345.t1.CDS1 | 2806952 | 2807476 |
| chr_3 | g345 | g345.t1 | exon | g345.t1.exon2 | 2807531 | 2808280 |
| chr_3 | g345 | g345.t1 | cds | g345.t1.CDS2 | 2807531 | 2808280 |
| chr_3 | g345 | g345.t1 | exon | g345.t1.exon3 | 2808342 | 2808415 |
| chr_3 | g345 | g345.t1 | cds | g345.t1.CDS3 | 2808342 | 2808415 |
| chr_3 | g345 | g345.t1 | exon | g345.t1.exon4 | 2809272 | 2809329 |
| chr_3 | g345 | g345.t1 | cds | g345.t1.CDS4 | 2809272 | 2809329 |
| chr_3 | g345 | g345.t1 | TSS | g345.t1 | NA | NA |
Sequences
>g345.t1 Gene=g345 Length=1407
ATGTTTCCTGATCATTCTCGGAATCATATTGAAGGACCAGTTGATATTGATGCACTTTGT
ACACTTTGGTACACCTTATTTAAATGGAATGTCACGCAATATTTGTTGAATTTGGAACCA
GCTGATGTTATGACAATTGGAAATCATGAGTTTGATCACGGTATTGATGGATTAGTACCA
TTTTTGGATCATATAAAAACACCTGTTGTTGTGTGTAATTTTGATGACTCATCTGAACCA
AAAATGCAAGGAAAATATACAAAATCAATTGTGTTAGAAAAAAATGGAAAGAAAATTGGA
GTCGTTGGTGTAACAACAACTCTTGCTACAGGAAATCGAGGAAAATTAAAAATTTTACCA
GAAGTCGAAAATGTTAAAAAAGAGGTTGACAACTTAGTCGAGAAAAGAATAAAAATTATC
ATCGTTCTCTCACATTCTGGCCTTGAAGTTGATCGTGAGATTGCAAAACATGGTGGAGAT
ATTGATATAATTGTTGGTGGTCATTCTCACTCATTTCTTTACTCTGGAACTCCTTCAATT
GGACCAGACACACCTGTAAGTGACTATCCAACAATTGAAGAGCAAGAAAATGGTAGAAAA
GTTCTAATAGTTCAAGCATCTGCTTATGCAAAATATCTTGGAAATATTACACTCTATTTT
GATGAAGATGGTGAAATTAAACACTATGAAGGTGCACCAATATTTTTGTCACATGATATC
GAGCAAGATCCTGAGATTATTAACAAATTAAAACCATGGAAAGAAGAACTTGATAAATAT
CAGAGCATGAAAATTGGATCAATAAATTTCTCACTTGATTTTTCATGTTTAGAACAAGAA
TGTGGAATTGGAAATTTAATAACTGATGCAATGGTTGATTCTTTTGTAAATGCAGCAGAT
TCGGGTGAATGGACAGCAGCTTCAATTGCTCTTTTAAATTCAAGAGGCATTCGGTCAACT
TTATCACGTGGTGACATCAATTATGCTGATTTAGTTTCTGTGCTACCTTTTGAAAATTAT
TATAATATTATTGAAATACCTGGCTCAACATTAAGAGAAGCTTTGGAATTTTCTGTCTCT
AATTCTAGTACGCTTGCAATTTTTCAAGTCTCAGGGATAAAAGTTACTTTTGACTTAAAA
AGAAAACCATATGATCGAATTGTTGAATTAAAAGTTTTATGTCAAGACTGTTTGATGCCA
AAATATGAAGATATTGATAAATCAAAAGATTATCGTGTTGTGATTTCAAATTATATAGCA
GAAGGTGGAGATGGCTATACAATGTTTCCTGCAGCTACAAGCTATACAGTGATAGGAATG
AGAGACATTGATGTACTTTCAGAATATATAAAAAAATATTCTCCAATAACTAAACCACTC
ATTCAGTCAAGGATTAAATTTTTGTGA
>g345.t1 Gene=g345 Length=468
MFPDHSRNHIEGPVDIDALCTLWYTLFKWNVTQYLLNLEPADVMTIGNHEFDHGIDGLVP
FLDHIKTPVVVCNFDDSSEPKMQGKYTKSIVLEKNGKKIGVVGVTTTLATGNRGKLKILP
EVENVKKEVDNLVEKRIKIIIVLSHSGLEVDREIAKHGGDIDIIVGGHSHSFLYSGTPSI
GPDTPVSDYPTIEEQENGRKVLIVQASAYAKYLGNITLYFDEDGEIKHYEGAPIFLSHDI
EQDPEIINKLKPWKEELDKYQSMKIGSINFSLDFSCLEQECGIGNLITDAMVDSFVNAAD
SGEWTAASIALLNSRGIRSTLSRGDINYADLVSVLPFENYYNIIEIPGSTLREALEFSVS
NSSTLAIFQVSGIKVTFDLKRKPYDRIVELKVLCQDCLMPKYEDIDKSKDYRVVISNYIA
EGGDGYTMFPAATSYTVIGMRDIDVLSEYIKKYSPITKPLIQSRIKFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g345.t1 | Gene3D | G3DSA:3.60.21.10 | - | 19 | 262 | 0.0e+00 |
| 13 | g345.t1 | Gene3D | G3DSA:3.90.780.10 | - | 264 | 468 | 0.0e+00 |
| 3 | g345.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 21 | 465 | 0.0e+00 |
| 4 | g345.t1 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 21 | 465 | 0.0e+00 |
| 8 | g345.t1 | PRINTS | PR01607 | Apyrase family signature | 135 | 152 | 0.0e+00 |
| 7 | g345.t1 | PRINTS | PR01607 | Apyrase family signature | 154 | 177 | 0.0e+00 |
| 5 | g345.t1 | PRINTS | PR01607 | Apyrase family signature | 201 | 221 | 0.0e+00 |
| 9 | g345.t1 | PRINTS | PR01607 | Apyrase family signature | 325 | 348 | 0.0e+00 |
| 6 | g345.t1 | PRINTS | PR01607 | Apyrase family signature | 404 | 423 | 0.0e+00 |
| 2 | g345.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 31 | 171 | 5.4e-06 |
| 1 | g345.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 273 | 429 | 0.0e+00 |
| 11 | g345.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 21 | 259 | 0.0e+00 |
| 10 | g345.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 265 | 460 | 0.0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K08693 (2’,3’-cyclic-nucleotide 2’-phosphodiesterase / 3’-nucleotidase / 5’-nucleotidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
