General information for g3482.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Iduronate 2-sulfatase.
Parent gene
- The parent gene g3482 is located on chr_3: 25849267..25850195 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3482 | g3482.t1 | TSS | g3482.t1 | 25848459 | 25848459 |
| chr_3 | g3482 | g3482.t1 | isoform | g3482.t1 | 25848500 | 25850195 |
| chr_3 | g3482 | g3482.t1 | exon | g3482.t1.exon1 | 25848500 | 25849159 |
| chr_3 | g3482 | g3482.t1 | cds | g3482.t1.CDS1 | 25848500 | 25849159 |
| chr_3 | g3482 | g3482.t1 | exon | g3482.t1.exon2 | 25849267 | 25850002 |
| chr_3 | g3482 | g3482.t1 | cds | g3482.t1.CDS2 | 25849267 | 25850002 |
| chr_3 | g3482 | g3482.t1 | exon | g3482.t1.exon3 | 25850059 | 25850195 |
| chr_3 | g3482 | g3482.t1 | cds | g3482.t1.CDS3 | 25850059 | 25850195 |
| chr_3 | g3482 | g3482.t1 | TTS | g3482.t1 | 25850349 | 25850349 |
Sequences
>g3482.t1 Gene=g3482 Length=1533
ATGAAATTGTGTATTTTTGTGATAATAGTTGTGAATTTCAATACAAATTGGTTTGCTGTT
GCTGCTACAAACAGGCCAAATGTGGTTTTTATCATGCTGGATGATTTTCGTGGAAACATT
CGGGCTCTAGGTGATGATTTGGCAGTGACACCAAATATGGACGCATTACTGTCAAAGAGC
TTCTACTTTTCATCTGTTTTTGCTCAGCAGGCACTTTGTGCTCCATCTCGGAATTCTATG
TTGACATCTCGAAGGCCGGATACTATTTTGACCTATAATTTTCAGCATTATTGGCGAACA
TTCGCTGGAAACTATACAACTTTGCCGCAATATTTTAAAGAGAATGGCTACCAAACAGAA
TCAATTGGAAAAATTTTTCATCATGGTCCTTCATCAAATAAAACTGATGATTATCCTTAC
AGTTGGTCTAATCCGACTTTCATTCCATCAACTCAAGAATATAGAAATCGACCAGTTTGC
AAAAATCAAGGCGATGATGAACTACATCGAAATTTACTTTGTCCAGTTATTGTTAAATTT
CAACCAGAACAAACACTTCCTGATATACAGTCAACAGAAGCAGCAAAAGAATTTTTAATG
GAAGCACAAGAGCCGTTTTTCTTAGGAATTGGCTTCTACAAGCCACACATACCTTTTAAG
TTTCCTTATGAGTATCTTAAGTATCATGACATCAATAACTTTCGCAAAGACTTTCTACGA
CCTTTTGATTTGCCTCAAGCTGCATGGAACCCATTCAATGATTTACGAGACCGTGATGAT
GTTAAAAGTCTCAATGTTAGCTATCCGTTTGGTCCTATACCAAGTGAATTTGCAGCTAAA
ATTCGACAGCATTACTATGCAAGTGTAACATATATTGATTCGTTGCTTGGTGAAATTTTG
AAAATGATTGATTTTACTAATACGATTGTCGTATTGACCAGTGACCACGGATGGAGTTGT
GGTGAGCATGGAGAATGGGCAAAATATAGCAATTATGAAGTGTCATTACGAGTCCCGTTA
ATAATTTTTGATCCAGAACGACCGCAATCGAAAATGCGACGCATCAATTCAATTGCTGAA
CTCATCGACGTTTTTCCTACACTCGTTGATATAGCTGGGTTACCAAAAGTGAGTAAATGC
AATAACACACTAGAGGATAATTTGACATGCACGGAAGGAAAGAGCCTGCACCACAAAATG
CTGAATGATGAAGAGAACAATGATGATAATGAAGTCGCATTCAGTCAATATCCGCGGCCT
GGAACTTATCCAACAAAAATACCCGATAGCGATCAACCAAAATTGAGAGAAATCAAAATT
ATGGGCTATACGATACGTACGAAAAGATTTCGATACACTGCCTGGATTAAATTTAACAAC
AAAAAATTTAAACGAAATTGGAATAAAATTTATGGTGAAGAACTTTATGACCATGATATT
GATGATAGTGAAAATATAAATCTTGTTCATCGTGCTGAATTTAGACAACTTAAGAATCAA
CTTAAGGAAAAATTAATGAAAAAATTTCCATGA
>g3482.t1 Gene=g3482 Length=510
MKLCIFVIIVVNFNTNWFAVAATNRPNVVFIMLDDFRGNIRALGDDLAVTPNMDALLSKS
FYFSSVFAQQALCAPSRNSMLTSRRPDTILTYNFQHYWRTFAGNYTTLPQYFKENGYQTE
SIGKIFHHGPSSNKTDDYPYSWSNPTFIPSTQEYRNRPVCKNQGDDELHRNLLCPVIVKF
QPEQTLPDIQSTEAAKEFLMEAQEPFFLGIGFYKPHIPFKFPYEYLKYHDINNFRKDFLR
PFDLPQAAWNPFNDLRDRDDVKSLNVSYPFGPIPSEFAAKIRQHYYASVTYIDSLLGEIL
KMIDFTNTIVVLTSDHGWSCGEHGEWAKYSNYEVSLRVPLIIFDPERPQSKMRRINSIAE
LIDVFPTLVDIAGLPKVSKCNNTLEDNLTCTEGKSLHHKMLNDEENNDDNEVAFSQYPRP
GTYPTKIPDSDQPKLREIKIMGYTIRTKRFRYTAWIKFNNKKFKRNWNKIYGEELYDHDI
DDSENINLVHRAEFRQLKNQLKEKLMKKFP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g3482.t1 | CDD | cd16030 | iduronate-2-sulfatase | 25 | 494 | 1.16093E-180 |
| 6 | g3482.t1 | Gene3D | G3DSA:3.40.720.10 | Alkaline Phosphatase | 25 | 509 | 1.0E-88 |
| 2 | g3482.t1 | PANTHER | PTHR45953 | IDURONATE 2-SULFATASE | 19 | 508 | 2.5E-167 |
| 1 | g3482.t1 | Pfam | PF00884 | Sulfatase | 26 | 373 | 3.9E-50 |
| 8 | g3482.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 9 | g3482.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 10 | g3482.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 11 | g3482.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 21 | - |
| 7 | g3482.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 510 | - |
| 14 | g3482.t1 | ProSitePatterns | PS00149 | Sulfatases signature 2. | 116 | 127 | - |
| 3 | g3482.t1 | SUPERFAMILY | SSF53649 | Alkaline phosphatase-like | 25 | 507 | 1.14E-88 |
| 5 | g3482.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 13 | g3482.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 4 | g3482.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004423 | iduronate-2-sulfatase activity | MF |
| GO:0008484 | sulfuric ester hydrolase activity | MF |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01136 (iduronate 2-sulfatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed