General information for g3657.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g3657 is located on chr_3: 27232393..27235509 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3657 | g3657.t1 | TTS | g3657.t1 | 27232344 | 27232344 |
| chr_3 | g3657 | g3657.t1 | isoform | g3657.t1 | 27232393 | 27235509 |
| chr_3 | g3657 | g3657.t1 | exon | g3657.t1.exon1 | 27232393 | 27233858 |
| chr_3 | g3657 | g3657.t1 | cds | g3657.t1.CDS1 | 27232393 | 27233858 |
| chr_3 | g3657 | g3657.t1 | exon | g3657.t1.exon2 | 27233924 | 27234179 |
| chr_3 | g3657 | g3657.t1 | cds | g3657.t1.CDS2 | 27233924 | 27234179 |
| chr_3 | g3657 | g3657.t1 | exon | g3657.t1.exon3 | 27234299 | 27234412 |
| chr_3 | g3657 | g3657.t1 | cds | g3657.t1.CDS3 | 27234299 | 27234412 |
| chr_3 | g3657 | g3657.t1 | exon | g3657.t1.exon4 | 27234484 | 27234865 |
| chr_3 | g3657 | g3657.t1 | cds | g3657.t1.CDS4 | 27234484 | 27234865 |
| chr_3 | g3657 | g3657.t1 | exon | g3657.t1.exon5 | 27234932 | 27235509 |
| chr_3 | g3657 | g3657.t1 | cds | g3657.t1.CDS5 | 27234932 | 27235509 |
| chr_3 | g3657 | g3657.t1 | TSS | g3657.t1 | 27235562 | 27235562 |
Sequences
>g3657.t1 Gene=g3657 Length=2796
ATGTGGTTAATTATAATTTTGTGCATTAGTGCTTATGTTCATGTGATAGAATGTGGTGAT
CCAAAAAGATATTCATACAAGAGTTTTGATGATATTGATGAAAATTTTGATCCTAGTTTT
ACTCCAAGAATTTTTGATGAATCGCTTTCATTTAGATTGCCTAATAACACAGTGCCAACT
CATTATGATTTGTTGCTTCTAACAGACATCCATTTACCTAATTTTGAATTTTTTGGGACT
GTCACAGTACACATTCGTGCTGTTGAAACAAGTCAATATATAGTTATGAATTACAAGCAA
CTGATAATTACGAATATAAACTTATTCGATTCAAATGGATCATTGATTGAAAGTGGTGTA
AATTATACACAGGCTGAAGTCACTGAATTATTAACAATTTTACCTCAAAATGAGCTTATT
GCTGATCAGGAATATCGTGTTACGATTAGTTATCGAGGTACATTAAGAACTGATCATTAT
GGTTTTTATCGTGCAAGTTACATAAATGAGCAAGGAAATCGAATATGGTACGCTACATCA
CAATTTCAAGCAACTGATGCCAGACACAGTATGCCCTGCTATGATGAACCACATTTACGA
ACAAGCTTTAGTATAAGAATTCGCCATCATCAAAGTTATATTGCTCTATCAAATATGCCT
GTACAATCTAGAACACCCGGACCAATTGCTAATTTTGTAATTACAACATTTGAAAAAATT
CCTTCAATGCAAACTTATTTATTGGTATTTACAATATCTGATTTTCATTTTGCTGAAGAT
ACGACAGTTGTTCCACCTCAAAGAGTTTACGCAAAACCCGAATCAATACGTAACCATGAG
TATGAGCTTGCTTTAAAAGTAAGCCCTGAAATAATGCTCACATGTGAAAATTTTTTCAAC
ATGTCCTATACTTTCCCTAAAATGGATCAAATAGCTGTTACTGAATTCCCTGCTGGAGCT
ATGGAGAACTGGGGTAGTGTTCTTTATCTCGAAAGATTTCTATTATTTGATCCACTGGTC
AGCACGACGCGCGATCATGAAAATATTTTGACGATAATTTCGCATGAGTTTGTGCATCAA
TGGATAGGAAACAAGGTTGTATTTCAATGGTGGACATACCTTTGGTTAAATGAAGCTTTT
GCTACACTCTATGAATATTACATGGTGCATTTAACTTATCCAGAAGAACGTTATGATGAT
TCCTTTTTGGTAGATGTATTGCAACGAGTATTGGAAATAGATGCTAATCCAAATATTAGA
CCAATGTCTTTATATGTTGAAGATCCTTGGTCTATTCTTATGCTTTTTGATTGGATAGCT
TATTCAAAAGGTGGAAGCGTTCTTTATACATTTATGAATGCTCTCGGTCAATCAACATGG
CAAAAAGGTTTACAGTACTATTTAACTGCACGCTACGACAACTATGCTAATCCTGGTCAT
CTTTATGAAAATTTACAAATTGCTGTTAATCAAGATTATCCTACAAATACACCAGACGTA
GCTGTTATGTTAGAATCTTGGGAATGGCAAAGTGGTTTTCCTATTGTTACAGTAAATTTC
CAAAATGATCACTTGATTCTTACACAAGAGCGATTTTTCTATAATACATCATTAACTTCA
GAAAATTTATGGTATATTCCAATTAACTATATATCTGCTACAAATCCCAATAGTGAAGAT
TCAACTTTTGATTTTTGGATGAGTTCAGAAGAAGTAATATTGCGAAATGAAAGTGCAAGA
AAATCATGGTTGGAAGATGATTGGTTAATTTTTAATATTAAACAAAATTATTATTATCGA
GTTAATTATGATTTGATGATGTGGAACCGAATCATTAAACAGTTAAATGAAGAAGATGAT
GGCTATAACGTGATTCACCTTCGAAATCGAGCACAGTTGATTGATGATTCTTATCATTTA
GCACGAGGAAATCGAATTGATTATTCAATTTTATTTAGTGTAATGGATTATCTCCGATAT
GAGACAGATTATATTCCTTGGGTCCCTGCAAATAGAGCCATAAATATTCTCAATCGATAT
CTTAGTGGTTCTATAGTTTACACAAAATATCAGGAATATATGAGATCAAATACAGCTAAA
TTATTTAATAAATTAGGTGTCAATTTTATTTCGAATGAACCTCGAGTTGATCGTTTTGCA
CGTTCAATTGCTATGAATCAAGCTTGTCGTGCTCAACTTCCAGAATGCCTTGAACAAGTT
AACAAAAAATTGATAAGACATTTAAGAAACAATGAACGAATTGAAACTGAGTATTTTACT
TCTACATATTGTAATGGTATGAGACAAGCTGATGGTTTCTTATTCATTGCTATGCAAAAT
AAACTAATTCAAAGTTCAATTCAAAGCGAAAGAAATGATATAATCAGTGGAATGGGATGT
GTACAAAATCAAGATTTAGTCTTCATCTTTCTTAATTTAGCCATTACTCCTGGTATTCAA
CTAACTTCGACTGAGAAACTTAGAATATTGACTTCACCAACAACAAATGGACCATCATCT
TTATCAAGTACTATTGAATTTGTAAATGTCTATTTTAGTCTTATTCAGCAAATAAGTTCT
AATGCTATTAGTAATGTTTTAACAAATATTGCCAACAATATAGCATCTGAAGAAGTTTTT
GATAAATATGACGAATTGTTGAGAACACTTACAATAAATCAAATTATCAGTGGACCAGAT
TATATTCAATACCTTGCCAATGGAAGAATAAATTTAAATTGGCAAGCTTCATATCTTGGA
CCTATTGCTGAATTTTTTGAACAATGGAAATTATAA
>g3657.t1 Gene=g3657 Length=931
MWLIIILCISAYVHVIECGDPKRYSYKSFDDIDENFDPSFTPRIFDESLSFRLPNNTVPT
HYDLLLLTDIHLPNFEFFGTVTVHIRAVETSQYIVMNYKQLIITNINLFDSNGSLIESGV
NYTQAEVTELLTILPQNELIADQEYRVTISYRGTLRTDHYGFYRASYINEQGNRIWYATS
QFQATDARHSMPCYDEPHLRTSFSIRIRHHQSYIALSNMPVQSRTPGPIANFVITTFEKI
PSMQTYLLVFTISDFHFAEDTTVVPPQRVYAKPESIRNHEYELALKVSPEIMLTCENFFN
MSYTFPKMDQIAVTEFPAGAMENWGSVLYLERFLLFDPLVSTTRDHENILTIISHEFVHQ
WIGNKVVFQWWTYLWLNEAFATLYEYYMVHLTYPEERYDDSFLVDVLQRVLEIDANPNIR
PMSLYVEDPWSILMLFDWIAYSKGGSVLYTFMNALGQSTWQKGLQYYLTARYDNYANPGH
LYENLQIAVNQDYPTNTPDVAVMLESWEWQSGFPIVTVNFQNDHLILTQERFFYNTSLTS
ENLWYIPINYISATNPNSEDSTFDFWMSSEEVILRNESARKSWLEDDWLIFNIKQNYYYR
VNYDLMMWNRIIKQLNEEDDGYNVIHLRNRAQLIDDSYHLARGNRIDYSILFSVMDYLRY
ETDYIPWVPANRAINILNRYLSGSIVYTKYQEYMRSNTAKLFNKLGVNFISNEPRVDRFA
RSIAMNQACRAQLPECLEQVNKKLIRHLRNNERIETEYFTSTYCNGMRQADGFLFIAMQN
KLIQSSIQSERNDIISGMGCVQNQDLVFIFLNLAITPGIQLTSTEKLRILTSPTTNGPSS
LSSTIEFVNVYFSLIQQISSNAISNVLTNIANNIASEEVFDKYDELLRTLTINQIISGPD
YIQYLANGRINLNWQASYLGPIAEFFEQWKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 25 | g3657.t1 | CDD | cd09601 | M1_APN-Q_like | 60 | 507 | 3.47641E-156 |
| 18 | g3657.t1 | Coils | Coil | Coil | 737 | 757 | - |
| 19 | g3657.t1 | Coils | Coil | Coil | 931 | 931 | - |
| 15 | g3657.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 48 | 255 | 6.8E-59 |
| 16 | g3657.t1 | Gene3D | G3DSA:1.10.390.60 | - | 262 | 512 | 2.8E-72 |
| 14 | g3657.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 513 | 603 | 2.6E-19 |
| 17 | g3657.t1 | Gene3D | G3DSA:1.25.50.20 | - | 604 | 928 | 3.6E-68 |
| 4 | g3657.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 46 | 915 | 1.3E-224 |
| 5 | g3657.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 46 | 915 | 1.3E-224 |
| 9 | g3657.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 188 | 203 | 2.4E-21 |
| 6 | g3657.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 237 | 252 | 2.4E-21 |
| 8 | g3657.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 316 | 326 | 2.4E-21 |
| 7 | g3657.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 352 | 367 | 2.4E-21 |
| 10 | g3657.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 371 | 383 | 2.4E-21 |
| 3 | g3657.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 58 | 247 | 7.8E-43 |
| 2 | g3657.t1 | Pfam | PF01433 | Peptidase family M1 domain | 284 | 504 | 1.8E-58 |
| 1 | g3657.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 588 | 889 | 2.7E-42 |
| 21 | g3657.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 22 | g3657.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 23 | g3657.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 24 | g3657.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 20 | g3657.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 931 | - |
| 12 | g3657.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 48 | 254 | 6.15E-47 |
| 11 | g3657.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 265 | 519 | 2.05E-57 |
| 13 | g3657.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11140 (aminopeptidase N)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
