General information for g3735.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Tyrosine-protein kinase Shark.
Parent gene
- The parent gene g3735 is located on chr_3: 27729569..27732788 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3735 | g3735.t1 | isoform | g3735.t1 | 27729569 | 27732788 |
| chr_3 | g3735 | g3735.t1 | exon | g3735.t1.exon1 | 27729569 | 27729593 |
| chr_3 | g3735 | g3735.t1 | cds | g3735.t1.CDS1 | 27729569 | 27729593 |
| chr_3 | g3735 | g3735.t1 | exon | g3735.t1.exon2 | 27730619 | 27731107 |
| chr_3 | g3735 | g3735.t1 | cds | g3735.t1.CDS2 | 27730619 | 27731107 |
| chr_3 | g3735 | g3735.t1 | exon | g3735.t1.exon3 | 27732138 | 27732172 |
| chr_3 | g3735 | g3735.t1 | cds | g3735.t1.CDS3 | 27732138 | 27732172 |
| chr_3 | g3735 | g3735.t1 | exon | g3735.t1.exon4 | 27732251 | 27732546 |
| chr_3 | g3735 | g3735.t1 | cds | g3735.t1.CDS4 | 27732251 | 27732546 |
| chr_3 | g3735 | g3735.t1 | exon | g3735.t1.exon5 | 27732611 | 27732788 |
| chr_3 | g3735 | g3735.t1 | cds | g3735.t1.CDS5 | 27732611 | 27732788 |
| chr_3 | g3735 | g3735.t1 | TSS | g3735.t1 | NA | NA |
| chr_3 | g3735 | g3735.t1 | TTS | g3735.t1 | NA | NA |
Sequences
>g3735.t1 Gene=g3735 Length=1023
ATGTATTTGAAGAAAGTTAAAAATAACTTGATTGAGTATTATAAGCACAATGAATCGAAT
CTCTGTACAAAATTAGGAAAGTTTGTAAAGAAAGGTCCACCACCATTAAAATATTGCAAA
TTAGGAAAAGCAAATTTGCTTCATCGTGCCACAAAGCATAATAATTTAAATGTTGTCAAA
GAAATTTTATCGACATCATATCGAAATTTAGATGCAAAAGATGAAAATGGAATGACAGCA
GTTCATTTAGCTGCAATAAATAAAGTCAGTGCTGAAATTATGAAATTGCTCATAGAAAAT
GGAAGTGCCTTAGCAAGTAGAGACTCAGTTGGAAATACACCACTGCATTATGCTTGTAAA
TTTGAATGCAAAGAAATGGTTGAACTTTTAATTGGTGCAAGTAAACCATTGATTTATGCT
AGAAACACTTTTACACACGAAGTGCCATTACATGAAGCTGCCAAAGTTGGAAATCTTGAA
ATTGTCAAAATTTTACTGCAAAATAATGCAGCAACGAATGGTTTGAACACTATACCATAC
TTTTTATTTGATAATTTTGTTTATAAAAAGGAAGAAAAAGAAATTGTAGAACCAAAAAAA
TCATTTTACTTTTATGAACCACCATCAACAATTTCTACACCATTATCAAGTTATAAAAGT
AGAGAAACAATTCCAATTACAGAAGAAATTGAAGAACATCGAGCGTATGTACCAGTGTAT
CAATCAATCGAACATAAACCTAAAATAAAGTCACTGCCATTACCAACGCCCACAAGTTAT
GTTGAGTTCAATGAACACAAATATAAAACTGAACATGAACATGAAAATAAAAATTACGAT
TTTGGATATCATGTGACAGATTATAAGAGTGGTAATAACTTTGGTCATACACAGTCAAAG
AAACAAAATGTCATAAATGGACAATATTCAATTTTGATGCCTGATGGAAGGATTCAAGTC
ACAAAATATATAGCAGATGATAGTGGATTCCATGCAGATATTAGTTATAAAACTATTCTT
TAA
>g3735.t1 Gene=g3735 Length=340
MYLKKVKNNLIEYYKHNESNLCTKLGKFVKKGPPPLKYCKLGKANLLHRATKHNNLNVVK
EILSTSYRNLDAKDENGMTAVHLAAINKVSAEIMKLLIENGSALASRDSVGNTPLHYACK
FECKEMVELLIGASKPLIYARNTFTHEVPLHEAAKVGNLEIVKILLQNNAATNGLNTIPY
FLFDNFVYKKEEKEIVEPKKSFYFYEPPSTISTPLSSYKSRETIPITEEIEEHRAYVPVY
QSIEHKPKIKSLPLPTPTSYVEFNEHKYKTEHEHENKNYDFGYHVTDYKSGNNFGHTQSK
KQNVINGQYSILMPDGRIQVTKYIADDSGFHADISYKTIL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g3735.t1 | Gene3D | G3DSA:1.25.40.20 | - | 15 | 196 | 1.2E-29 |
| 4 | g3735.t1 | PANTHER | PTHR24203:SF45 | ANKYRIN REPEAT FAMILY PROTEIN | 46 | 131 | 1.5E-29 |
| 6 | g3735.t1 | PANTHER | PTHR24203 | ANKYRIN REPEAT FAMILY PROTEIN | 46 | 131 | 1.5E-29 |
| 3 | g3735.t1 | PANTHER | PTHR24203:SF45 | ANKYRIN REPEAT FAMILY PROTEIN | 61 | 177 | 1.5E-29 |
| 5 | g3735.t1 | PANTHER | PTHR24203 | ANKYRIN REPEAT FAMILY PROTEIN | 61 | 177 | 1.5E-29 |
| 2 | g3735.t1 | Pfam | PF12796 | Ankyrin repeats (3 copies) | 47 | 133 | 3.1E-15 |
| 1 | g3735.t1 | Pfam | PF00379 | Insect cuticle protein | 279 | 330 | 3.2E-9 |
| 12 | g3735.t1 | ProSitePatterns | PS00233 | Chitin-binding type R&R domain signature. | 307 | 332 | - |
| 14 | g3735.t1 | ProSiteProfiles | PS50297 | Ankyrin repeat region circular profile. | 42 | 177 | 29.998 |
| 15 | g3735.t1 | ProSiteProfiles | PS50088 | Ankyrin repeat profile. | 76 | 109 | 10.74 |
| 16 | g3735.t1 | ProSiteProfiles | PS50088 | Ankyrin repeat profile. | 145 | 177 | 11.087 |
| 17 | g3735.t1 | ProSiteProfiles | PS51155 | Chitin-binding type R&R domain profile. | 276 | 340 | 14.302 |
| 9 | g3735.t1 | SMART | SM00248 | ANK_2a | 42 | 72 | 9.9 |
| 8 | g3735.t1 | SMART | SM00248 | ANK_2a | 76 | 106 | 0.028 |
| 11 | g3735.t1 | SMART | SM00248 | ANK_2a | 110 | 140 | 0.055 |
| 10 | g3735.t1 | SMART | SM00248 | ANK_2a | 145 | 174 | 0.068 |
| 7 | g3735.t1 | SUPERFAMILY | SSF48403 | Ankyrin repeat | 43 | 176 | 3.8E-29 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0042302 | structural constituent of cuticle | MF |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
